SIGMA| 人血清白蛋白

PRODUCTNO.

PREPARATION/PURIFICATION

SPECIAL CHARACTERISTICS

A 1653

Fraction V1

From human serum.

Powder

96-99% by agarose gel (remainder mostly globulins)

A 2817

Fraction V1

Powder

96-99% by agarose gel (remainder mostly globulins)

Produced specifically for bulk usage.

A 1887

Fraction V1, heat step2 Prepared from A 1653.

Powder

³96% by agarose gel

Essentially fatty acid free (∼0.005%)

A 9511

1× crystallized ａnd lyophilized.

Prepared from A 1653 using method IV of Cohn, et al.3

Powder

97-99% by agarose gel

A 8763

Prepared from A 1653.4

Powder

Essentially globulin free

A 3782

Prepared from A 8763.4

Powder

Essentially fatty acid free (∼0.005%) Essentially globulin free

A 4327

Prepared from A 1653.

Powder

³96% by agarose gel

Essentially protease free (<0.0001 u/mg protein) Essentially alkaline phosphatase free (0.001 u/mg protein) Essentially peroxidase free (<0.001 u/mg protein)

A 3173

Prepared from A 2817.

Powder

Low endotoxin content (²0.1 ng/mg protein)

A 6784

Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled.

10% Solution

A 6909

Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled.

30% Solution

A 9080

From human plasma. Contains approx. 0.85% NaCl. Aseptically filled.

30% Solution Protease free.

A 2830

From human serum.

Solution in 40 mM potassium phosphate, pH 7.0

14C-Methylated

5-50 ìCi per mg protein

Residues         39    15   30   22  60   23  25

PHYSICAL  PROPERTIES:5

12 63

35  39   6   8  61 18     30  16  58  1              23

Sedimentation constant, S20,W  X  1013

4.6 (monomer), 6.5 (dimer)

Diffusion constant, D20,W  X 107

6.1

Partial specific volume, V20

0.733

Intrinsic viscosity, ç

0.042

Frictional ratio, f/f0

1.28

Overall dimensions, Å

38 X 150

Isoelectric point (Ã/2 = 0.15)

4.7

Isoionic point (Ã/2 = 0)

5.2

Electrophoretic mobility, pH 8.6, Ã/2 = 0.15

-5.9

Refractive index increment (578 nm) X   10-3

1.89

Optical absorbance, A279 nm  (1  gram/liter)

0.531

Mean residue rotation,  [m＇]233

8590

Mean residue ellipticity

17 [è]209 nm; 16 [è]222 nm

Estimated á-helix, %

48

Estimated â-form, %

15

SOLUBILITY / SOLUTION STABILITY: