兔血栓素B2(TXB2)ELISA试剂盒厂家

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【兔血栓素B2(TXB2)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【兔血栓素B2(TXB2)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【兔血栓素B2(TXB2)ELISA试剂盒厂家】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【兔血栓素B2(TXB2)ELISA试剂盒厂家】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9238651  Interference suppression filter F11.126/           CNY                              【兔血栓素B2(TXB2)ELISA试剂盒厂家】

9238641  Fan, power board ａnd casing  this is a 5.25" fan that is mounted horizontally underneath the centrifuge.  CNY                            

9238637  Diode BYW 35           CNY                             

9240970  Motor, x, sample tracking, Qsym              CNY                             

9240965  X-Ring, pipetting system, Qsym                 CNY                             

9240683  Spring, Elevator Pickup Head, AP196/98           CNY                             

9240814  #Agreement, EZ1 DSP, PM, 1YR                CNY                             

9240788  Update, Pump Pulley/Motor, AP98          CNY                             

6000-5008        RCS Reagent Troughs                CNY                             

6000-5004        Sealing Film Dispenser              CNY                             

9241768  QIAxtractor, Basic Agreement         The Basic Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Basic Cover Agreement, there is a response time of 5 working days.   CNY                             

9241656  Transfer Rack Plate, AP             CNY                             

9241630  Shaker Adjustment Kit, AP                CNY                             

9241367  Damper, door, EZ1a                   CNY                             

9240759  Update, Sliding Door Latch, AP98             CNY                             

9243548  Pyro Q96 ID, PM Service "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242766  Cogwheel, x-drive, portal, Qsym               CNY                              【兔血栓素B2(TXB2)ELISA试剂盒厂家】

9242710  Validation Support, 2d, QIAgility      The QIAGEN QIAgility Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAgility system ａnd software, review the User Manual, ａnd practical hands-on operation. The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which processes (quant setup, normalization, PCR setup, CE setup) ａnd kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation.     CNY                             

9242253  Cable, x, portal AS, Qsym                   CNY                             

9242234  RGQ, IQ/OQ Service         "Instrument qualification support service for the Rotorgene Q ａnd Rotorgene 6000. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).

During the service, the compliance with the manufacturer＇s installation requirements is testified ａnd the performance verification & dynamic calibration of the Rotorgene Q is performed." CNY                             

9242172  Repair fee, Depot Repair, TissueLyser II           CNY                             

5000-1403        Bumpers Side, VTX2                   CNY                             

5000-1401        Guide Rack Back Asmbly, VTX2                  CNY                             

5000-1311        Capacitor 8UF, SKR1                  CNY                             

5000-1273        Door Damper Assembly, DML2                 CNY                             

5000-1258        Y Optical Sensor CMPLT, DML2                  CNY                             

5050-1074 V.1 CD Rom Manual - LumiCheck Plate          CNY                             

5050-1069 V.1 CD Rom Manual - Rotary Shaker I, 240V                   CNY                             

6000-5001        Costar Plate Lids                CNY                             

6000-3120        Plate Washer I - WasteReserv.CapAssembly             CNY                             

5000-1420        Driveplate Asmly 099A, VTX2           CNY                             

5127-1220        hc2 Sample Conversion Kit                CNY                             

QY-x1744 人鼻咽癌细胞（高分化）     CNE-1                                            

QY-x1745 人鼻咽癌细胞         SUME-a                                        

QY-x1746 人鼻咽癌细胞         HNE-2                                            【兔血栓素B2(TXB2)ELISA试剂盒厂家】

QY-x1747 人慢性髓原白血病细胞         K562                                              

QY-x1748 人组织细胞淋巴瘤细胞         U937                                             

QY-x1749 人红白细胞白血病细胞Erythroleukemia    HEL                                              

QY-x1750 人急性T淋巴母细胞白血病细胞          MOLT-4                                         

QY-x1751 人急性早幼粒白血病细胞（悬浮）     NB4                                      

QY-x1752 人骨肉瘤细胞         SW1353                                        

QY-x1753 人白血病细胞         Daudi                                            

QY-x1754 转染了tk基因的SW038-C2细胞 SW038-C2-tk                                        

QY-x1755 人星形胶质细胞瘤细胞        SHC-44                                          

QY-x1756 人神经胶质瘤细胞         BT-325                                          

QY-x1757 人XG恶性胶质瘤细胞  SF-295                                           

QY-x1758 人神经母细胞瘤细胞     SK-N-SH                                        

QY-x1759 人宫颈癌细胞         Hela                                     

QY-x1760 人宫颈癌细胞         SiHa                                     

QY-x1761 人乳腺腺癌细胞     MCF-7                                          

2QY-x1762        人膀胱变移细胞癌细胞         T-24                                       【兔血栓素B2(TXB2)ELISA试剂盒厂家】

QY-x1763 人纤维肉瘤细胞     HT-1080                                       

QY-x1764 人肝静脉内皮细胞         ED-25                                            

QY-x1765 肠系膜下腔动脉血管内皮细胞     Ealy926                                        

QY-x1766 人视网膜色素上皮细胞         D407                                             

QY-x1767 人胚肺成纤维细胞         HLF                                               

QY-x1768 人肝星状细胞         HSC                                              

QY-x1769 人正常胎肝细胞     L-o2                                      

QY-x1770 逆转录病毒包装用的人胚胎成纤维细胞     PA317                                           

QY-x1771 转染入tk基因的PA317细胞         PA317-tk                                             

QY-x1772 转染入GFP基因的PA317细胞（GFP-绿色荧光蛋白）      PA317-GFP                                           

QY-x1773 人胚肾细胞     HEK-293