兔吡啶交联物(PY)ELISA试剂盒厂家

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【兔吡啶交联物(PY)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【兔吡啶交联物(PY)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【兔吡啶交联物(PY)ELISA试剂盒厂家】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【兔吡啶交联物(PY)ELISA试剂盒厂家】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9020027  Sensor, solenoid, 170mm, EZ1a                 CNY                              【兔吡啶交联物(PY)ELISA试剂盒厂家】

9019500  UV light system, CAS                  CNY                             

9019499  ReagBlock, 8x1.5ml/2ml/1.5ml Ta/Flat/Ta                CNY                             

9241225  QIAcube, Inspection Service    "The Annual Inspection Service, QIAcube is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist. During the half day-long procedure, all application-critical modules of the QIAcube are inspected ａnd tested. Performed tests ａnd test results are documented in a Visit Report."  CNY                             

9241218  HC2, Full Agreement, no PM   "Service response time (on-site repair):not applicable

Periodic inspection/maintenance: no, not included

Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage

For defect DML, after customer calling QIAGEN technical service hotline before 12 am:

- A special package containing a replacement unit shall be sent to the customer within 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement"         CNY                             

9241182  Belt, Y, QX                  CNY                             

9238820  Rect. carrier for 2 tubes 50 ml                   CNY                             

9233269  Motor, X, BR8000             CNY                             

9237165  Power Supply XYP              CNY                             

9233156  Power Cord, USA               CNY                             

9237160  Cover LiquiChip                  CNY                             

9237117  Upgrade App., DyeEx 96          CNY                             

9237115  Upgrade App., Ni-NTA Beads            CNY                             

9237100  Calibrate BR, on-site                  CNY                             

9237091  Training, BR On-site Introductory    The On-Site QIAGEN BioRobot Systems Introductory Training covers up to 16 hours of training time to refresh current users or introduce new users to the QIAGEN BioRobot Systems hardware ａnd QIAsoft Operating System software.  Participants are given an overview of the hardware system ａnd maintenance/troubleshooting procedures before exploring the three software environments of the QIAsoft Operating System.  Each software environment is explained in detail, ａnd users are taken step by step through each section with explanations on how to best adjust or write new protocols for their BioRobot System.  An introduction to teaching new ａnd exisiting slots ａnd racks is followed by writing a new protocol which uses these with the three available pipetting options, pooling, distributing ａnd transfer.  Users are encouraged to write protocols in the proper format creating prompts, flushes, ａnd correct pipetting parameters.  The training concludes asprotocols are tested ａnd run reinforcing concepts ａnd demonstrating success.   CNY                             

9236407  Storage Platform TW I, microplate          CNY                             

9236364  Sheath Filter             CNY                              【兔吡啶交联物(PY)ELISA试剂盒厂家】

9232933  Microplate Reader(Agglutination)Module                CNY                             

9232916  Card, liquid detection 1.02                CNY                             

9232910  Board, Z1 motor                CNY                             

9236351  Tubing, Valve to injection needle              CNY                             

9236326  Sheath Pressure Sensor             CNY                             

9235760  Seal, O-ring, 3mm             CNY                             

9230926  Motor Connecting Ground Cable              CNY                             

9230545  Wanne fuer Abschirmung Plato 1xxx                 CNY                             

9235700  Power Cable 24V, RCS1             CNY                             

9230291  Tubing Guide (periscope)          CNY                             

211011     QuantiTect Virus Kit (50)          CNY                             

979017     MOTT 16S Primer 2 For Sequencing                  CNY                             

59824       EpiTect Fast DNA Bisulfite Kit (50)            CNY                             

206246     Type-it Microsatellite PCR Kit (2000)                 CNY                             

9012742  Syringe pump outlet tubing (4.7 m)                   CNY                             

EK0906     hTLR2-ELISA     人TLR2ELISA试剂盒      Human TLR2 ELISA Kit     96 tests                      

EK0907     hTLR3-ELISA     人TLR3ELISA试剂盒      Human TLR3 ELISA Kit     96 tests                      

EK0928     hTF/F3-ELISA    人组织因子/F3ELISA试剂盒 Human Tissue factor/F3 ELISA Kit   96 tests                      

EK0309     mBDNF-ELISA  小鼠脑源性神经生长因子ELISA试剂盒      Mouse BDNF ELISA Kit     96 tests                      

EK0313     mBMP-2-ELISA         小鼠BMP-2ELISA试剂盒        Mouse BMP-2 ELISA Kit   96 tests                       【兔吡啶交联物(PY)ELISA试剂盒厂家】

EK0326     mEGF-ELISA     小鼠表皮生长因子ELISA试剂盒  Mouse EGF ELISA Kit        96 tests                      

EK0333     mEPO-ELISA     小鼠促红细胞生成素ELISA试剂盒      Mouse EPO ELISA Kit        96 tests                      

EK0336     mFAS-ELISA      小鼠凋亡相关因子ELISA试剂盒  Mouse FAS ELISA Kit         96 tests                      

EK0351     mFN-ELISA        小鼠胚胎纤维连接蛋白ELISA试剂盒  Mouse Fibronectin ELISA Kit   96 tests                      

EK0361     mG-CSF-ELISA  小鼠粒细胞集落刺激因子ELISA试剂盒      Mouse G-CSF ELISA Kit     96 tests                      

EK0365     mGM-CSF-ELISA       小鼠粒细胞巨噬细胞刺激因子ELISA试剂盒      Mouse GM-CSF ELISA Kit 96 tests                      

EK0371     mICAM-1-ELISA        小鼠细胞间粘附分子ELISA试剂盒      Mouse ICAM-1 ELISA Kit 96 tests                      

EK0375     mIFN-γ-ELISA          小鼠IFN-γELISA试剂盒        Mouse IFN-γ ELISA Kit  96 tests                      

EK0378     mIGF-1-ELISA   小鼠IGF-1ELISA试剂盒 Mouse IGF-1 ELISA Kit     96 tests                      

EK0381     mIGF-2-ELISA   小鼠IGF-2 ELISA试剂盒         Mouse IGF-2 ELISA Kit     96 tests                      

EK0383     mIGFBP-1-ELISA       小鼠IGFBP-1ELISA试剂盒     Mouse IGFBP-1 ELISA Kit          96 tests                      

EK0387     mIGFBP-3-ELISA       小鼠IGFBP-3ELISA试剂盒     Mouse IGFBP-3 ELISA Kit          96 tests                      

EK0391     mIL-1α-ELISA 小鼠IL-1αELISA试剂盒         Mouse IL-1α ELISA Kit  96 tests                      

EK0394     mIL-1α-ELISA 小鼠IL-1αELISA试剂盒         Mouse IL-1α ELISA Kit  96 tests                      

EK0398     mIL-2-ELISA      小鼠IL-2ELISA试剂盒    Mouse IL-2 ELISA Kit        96 tests                      

EK0403     m IL-3 -ELISA    小鼠IL-3ELISA试剂盒    Mouse IL-3 ELISA Kit        96 tests                       【兔吡啶交联物(PY)ELISA试剂盒厂家】

EK0405     m IL-4-ELISA     小鼠IL-4 ELISA试剂盒   Mouse IL-4 ELISA Kit        96 tests                      

EK0408     m IL-5-ELISA     小鼠IL-5ELISA试剂盒    Mouse IL-5 ELISA Kit        96 tests                      

EK0411     m IL-6-ELISA     小鼠IL-6ELISA试剂盒    Mouse IL-6 ELISA Kit        96 tests                      

EK0417     mIL-10-ELISA   小鼠IL-10ELISA试剂盒  Mouse IL-10 ELISA Kit      96 tests                      

EK0422     mIL-12(p70)-ELISA   小鼠IL-12(p70)ELISA试剂盒 Mouse IL-12(p70) ELISA Kit      96 tests                      

EK0431     mIL-17-ELISA   小鼠IL-17ELISA试剂盒  Mouse IL-17 ELISA Kit      96 tests                      

EK0436     mLaminin-ELISA       小鼠层粘连蛋白ELISA试剂盒      Mouse Laminin ELISA Kit 96 tests                      

EK0438     mLeptin-ELISA 小鼠瘦蛋白ELISA试剂盒      Mouse Leptin ELISA Kit    96 tests                      

EK0440     mLeptinR-ELISA        小鼠瘦蛋白受体ELISA试剂盒      Mouse Leptin receptor ELISA Kit     96 tests