兔吡啶交联物(PY)ELISA试剂盒现货

价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供最优质产品。

【兔吡啶交联物(PY)ELISA试剂盒现货】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，最好做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请最后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【兔吡啶交联物(PY)ELISA试剂盒现货】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【兔吡啶交联物(PY)ELISA试剂盒现货】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【兔吡啶交联物(PY)ELISA试剂盒现货】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9012736  Peristaltic pump outlet tubing (1.0 m)              CNY                              【兔吡啶交联物(PY)ELISA试剂盒现货】

9012725  Syringe pump outlet for Plato           CNY                             

979001     PyroMark Q96 Plate (100)        "For sample analysis of DNA template prepared with magnetic beads for ID.

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9012706  Base, auto vac, BR8000  This is the robovac base for the BR8000 I. There are 5 contacts.   CNY                             

133240     NeXtalStock Sodium hydroxide (200)                 CNY                             

133233     NeXtalStock Sodium cacodyl. pH 7.5 (100)                CNY                             

9002195  RGQ 2plex, PC, PrioPLUS, basic, no PM   "RGQ 2plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

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9002039  Rotor-Gene Q MDx 5plex HRM, PrioPLUS       "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

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Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1 PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

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All cost covered (shipping, labor, parts)" CNY                             

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971540     therascreen® UGT1A1 Pyro Kit (24)                  CNY                             

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51504       QIAamp DNA Stool Mini Kit (50)      For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)  CNY                             

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922920     LiquiChip Filter Microplates (10)               CNY                             

292023     mericon Cattle (24)           CNY                             

292013     mericon Pig Kit (24)          CNY                              【兔吡啶交联物(PY)ELISA试剂盒现货】

9001903  QIAgility HEPA/UV (PC) Priority        QIAgility HEPA/UV (PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133122     NeXtalStock Potassium citrate (200)                 CNY                             

963141     QIAquick 96 PCR BioRobot Kit (4)    For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads  CNY                             

133193     NeXtalStock di-Potassium phosphate (200)               CNY                             

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9015329  O-rings, Norpren tube fixing, M48 (2)               CNY                             

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239095     GelPilot 1 kb Plus Ladder (100)         Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp         CNY                             

133127     NeXtalStock Sodium citrate (200)             CNY                             

133126     NeXtalStock TRIS-HCl pH 8.5 (200)           CNY                             

133008     NeXtalStock 2-Propanol (200)           CNY                             

133007     NeXtalStock MES sod. salt untit. (200)              CNY                             

9011949  Board, X-main           CNY                             

EK0445     mMCSF-ELISA  小鼠巨噬细胞集落刺激因子ELISA试剂盒  Mouse M-CSF ELISA Kit    96 tests                      

EK0460     mMMP-2-ELISA        小鼠MMP-2ELISA试剂盒      Mouse MMP-2 ELISA Kit 96 tests                      

EK0462     mMMP3-ELISA         小鼠MMP-3ELISA试剂盒      Mouse MMP-3 ELISA Kit 96 tests                      

EK0466     mMMP-9-ELISA        小鼠MMP-9ELISA试剂盒      Mouse MMP-9 ELISA Kit 96 tests                      

EK0470     mNGF-ELISA     小鼠NGF/NGFβELISA试剂盒       Mouse NGF/NGFβ ELISA Kit 96 tests                      

EK0473     mNT3-ELISA     小鼠Neurotrophin-3 ELISA试剂盒         Mouse Neurotrophin-3 ELISA Kit     96 tests                      

EK0483     mOPN-ELISA    小鼠骨桥素Elisa试剂盒ELISA试剂盒 Mouse OPN ELISA Kit       96 tests                       【兔吡啶交联物(PY)ELISA试剂盒现货】

EK0486     mPDGF-AB-ELISA     小鼠血小板衍生生长因子ABELISA试剂盒 Mouse PDGF-AB ELISA Kit        96 tests                      

EK0495     mRantes-ELISA         小鼠趋化因子ELISA试剂盒  Mouse Rantes ELISA Kit  96 tests                      

EK0504     mSELL-ELISA    小鼠L选择素Elisa试剂盒    Mouse L-Selectin ELISA Kit       96 tests                      

EK0506     mSELP-ELISA    小鼠P选择素Elisa试剂盒    Mouse P-Selectin ELISA Kit      96 tests                      

EK0515     mTGF        小鼠转化生长因子β1ELISA试剂盒     Mouse TGFβ1 ELISA Kit 96 tests                      

EK0527     mTNF-ELISA     小鼠TNFαELISA试剂盒        Mouse TNFα ELISA Kit  96 tests                      

EK0538     mVCAM-ELISA 小鼠血管细胞粘附分子-1ELISA试剂盒        Mouse VCAM-1 ELISA Kit         96 tests                      

EK0541     mVEGF-ELISA   小鼠血管内皮生长因子ELISA试剂盒  Mouse VEGF ELISA Kit     96 tests                      

EK0562     mECad-ELISA   小鼠钙粘着蛋白ELISA试剂盒      Mouse E-Cadherin ELISA Kit    96 tests                      

EK0577     mGp130-ELISA          小鼠Gp130/IL6STELISA试剂盒      Mouse Gp130/IL6ST ELISA Kit 96 tests                      

EK0582     mRes-ELISA      小鼠抵抗素ELISA试剂盒      Mouse Resistin ELISA Kit 96 tests                      

EK0664     mBAFF-ELISA   小鼠BAFFELISA试剂盒  Mouse BAFF ELISA Kit      96 tests                      

EK0668     mPCad-ELISA   小鼠P-CadherinELISA试剂盒         Mouse P-Cadherin ELISA Kit    96 tests                      

EK0703     mCD40-ELISA   小鼠CD40/TNFRSF5ELISA试剂盒  Mouse CD40/TNFRSF5 ELISA Kit      96 tests                      

EK0708     mCD80-ELISA   小鼠B7-1/CD80ELISA试剂盒 Mouse B7-1/CD80 ELISA Kit     96 tests                      

EK0717     mCTLA4-ELISA 小鼠CTLA4ELISA试剂盒         Mouse CTLA4 ELISA Kit    96 tests                       【兔吡啶交联物(PY)ELISA试剂盒现货】

EK0780     mIL7-ELISA       小鼠IL-7 ELISA试剂盒   Mouse IL-7 ELISA Kit        96 tests                      

EK0808     mCSF1R-ELISA 小鼠CSF1R/M-CSFRELISA试剂盒  Mouse CSF1R/M-CSFR ELISA Kit       96 tests                      

EK0830     mRANK-ELISA  小鼠Receptor Activator of NF-κB (RANK)ELISA试剂盒       Mouse Receptor Activator of NF-κB (RANK) ELISA Kit  96 tests                      

EK0866     mIL23-ELISA     小鼠IL-23ELISA试剂盒  Mouse IL-23 ELISA Kit      96 tests                      

EK0874     mPECAM-ELISA        小鼠 PECAM-1/CD31ELISA试剂盒         Mouse PECAM-1/CD31 ELISA Kit      96 tests                      

EK0925     mDKK-1-ELISA 小鼠DKK1ELISA试剂盒 Mouse DKK1 ELISA Kit     96 tests                      

EK0308     rBDNF-ELISA    大鼠脑源性神经生长因子ELISA试剂盒      Rat BDNF ELISA Kit 96 tests