猪活化素A(ACV-A)ELISA 试剂盒厂家

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【猪活化素A(ACV-A)ELISA 试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【猪活化素A(ACV-A)ELISA 试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【猪活化素A(ACV-A)ELISA 试剂盒厂家】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【猪活化素A(ACV-A)ELISA 试剂盒厂家】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9240692  Fan, 12Vdc, PC-104 cover, AP196/98                  CNY                              【猪活化素A(ACV-A)ELISA 试剂盒厂家】

51331       QIAamp 96 DNA QIAcube HT Kit (5)                  CNY                             

74171       RNeasy 96 QIAcube HT Kit (5)          CNY                             

180994     GeneRead Adapter L Set 12-plex (72)               CNY                             

9002140  careHPV test Luminometer              CNY                             

9230355  Washstation P1xxx            CNY                             

9235295  Sound absorber (muffler)                   CNY                             

9235277  Board, HS shaker, BR8000                 CNY                             

9230202  Safety shield for size1                CNY                             

9234858  Toubing coupling piece (washer tubing)           CNY                             

9238675  Operating mechanism,  left mech. lock                   CNY                             

9238655  Computer board cable, 3-channel            CNY                             

9238633  Grey wedge (long)   " This is a thin plastic sheet with a printed gradient of dots that is held by a bracket on the motor so that it is situated in the u shape of the sensor.

SIGMA sensor"         CNY                             

9238622  Main board Zent3, no Eprom (>SN85353)                 CNY                             

9241891  QIArent&go - Rent out fee Rotor-Gene           CNY                             

9241823  Pyro Q96 ID, Full Agreement   The Full Cover Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.         CNY                             

9241816  Pyro Q96 MD, PM Agreement                   CNY                              【猪活化素A(ACV-A)ELISA 试剂盒厂家】

9241807  Pyro Q96 ID, Installation Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements         CNY                             

9241125  Thermal Joint Compound                   CNY                             

9240978  PCB, connector, sample tracking, Qsym           CNY                             

9240947  Encoder, x, portal, Qsym          CNY                             

9234821  Manual, user, QS 4.0, US          CNY                             

9240785  Reagent #1 Manifold Update, AP98                  CNY                             

9241784  QIAxcel, Training      "The Training, QIAxcel is a 2 days on-site service for up to four QIAxcel users, conducted by a QIAGEN Field Service Specialist. It includes an introduction to operation of the QIAxcel system hardware ａnd the usage of the BioCalculator software. It covers the preparation of sample material, run preparation, data acquisition, ａnd data evaluation. The training is performed using standardized sample material; customer samples may be processed upon request.

All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. Training certificates for participants can be issued upon request."       CNY                             

9243544  Autopure, PM Service      "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242713  Add. Unit, Validation Support, 2d    Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Guided or Accelerated validation service catalog no(s). Scope of work defined from the primary unit utilized in "Guided Validation Support" or "Accelerated Validation Support".  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.        CNY                             

9242545  Quick Disconnect O-ring set, LC                 CNY                             

9242337  Tool, calibration, AS, Qsym                CNY                             

5000-1303        Potentiometer Speed, SKR1              CNY                             

5000-1283        DML 2000 - Power Cord, 220V B Type (IT)                CNY                            

5000-1279        Led Assembly, DML2                  CNY                             

5000-1269        Optical Sensor New STY, DML2                  CNY                             

QY-x1492 大鼠心肌细胞H9c2(2-1)                                                      

QY-x1493 大鼠肾小球系膜细胞EC (HBZY－1)HBZY－1                                                     

QY-x1494 绿猴恒河猴肾细胞LLC-MK2                                               

QY-x1495 牛肾细胞MDBK (NBL-1)                                                       

QY-x1496 貂肺上皮细胞Mv.1.Lu(NBL-7)                                                     

QY-x1497 小鼠脑神经瘤细胞Neuro-2a [N2a; Neuro-2a]                                                    

QY-x1498 大鼠肺泡巨噬细胞NR8383                                                

QY-x1499 负鼠肾细胞OK                                                     

QY-x1500 小鼠骨髓瘤细胞P3/NSI/1-Ag4-1 [NS-1]                                              

QY-x1501 小鼠骨髓瘤细胞P3X63Ag8.653                                                  

QY-x1502 猪髋动脉内皮细胞PIEC                                                       

QY-x1503 鼠逆转录酶病毒包装细胞PT67                                                 

QY-x1504 中国仓鼠仓鼠肺细胞R1610                                               

QY-x1505 绿猴猴脉络膜-视网膜(内皮)细胞RF/6A                                                     

QY-x1506 大鼠雪旺细胞RSC96                                                     【猪活化素A(ACV-A)ELISA 试剂盒厂家】

QY-x1507 猪睾丸细胞系(ST)ST                                                     

QY-x1508 蝙蝠肺细胞Tb 1 Lu                                                       

QY-x1509 小鼠B细胞杂交瘤细胞W6/32                                                    

QY-x1510 鼠纤维肉瘤细胞系WEHI 164                                                      

QY-x1511 小鼠B淋巴细胞WEHI 231                                                 

QY-x1512 大鼠垂体细胞RPC                                               

QY-x1513 小鼠垂体细胞MPC                                                       

QY-x1514 兔子垂体细胞                                                       

QY-x1515 狗垂体细胞                                                  

QY-x1516 鼠肝癌细胞系MM45T.Li                                                      

QY-x1517 小鼠成纤维细胞系McCoy                                                   

QY-x1518 大鼠肝癌细胞（乳腺癌细胞接种到肝脏成瘤）Walker-256                                                    

QY-x1519 大鼠肝癌细胞（wistar 大鼠）CBRH7919                                                 

QY-x1520 小鼠结肠癌C26                                                    

QY-x1521 稳定转染了neor和LIF基因的STO细胞株SNL                                                

QY-x1522 小鼠艾氏腹水瘤细胞ECA                                                    【猪活化素A(ACV-A)ELISA 试剂盒厂家】

QY-x1523 褐鼠胰岛素瘤上皮细胞RIN-m                                                   

QY-x1524 小鼠杂交瘤细胞ST2/o