猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】样本保存条件：

1.2-8℃冰箱最多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，最好还是用PBS

【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

764114    PAXgene Bone Marrow RNA Tubes (50)            CNY                            【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】

679913    ipsogen RT Kit (33)              CNY                           

203643    HotStarTaq Plus Master Mix Kit (250)  "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."     CNY                           

201225    Taq PCR Core Kit (1000)      "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

201203    Taq DNA Polymerase (250)   "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

180922    GeneRead Adapter L Set 1plex (12)             CNY                           

180912    GeneRead Adapter I Set 1plex (12)              CNY                           

159214    QIAcard FTA Indicator Four Spots (25)             CNY                           

159201    QIAcard FTA One Spot (100)              CNY                           

9235523  Needle cleaning assembly P/N 4567-01        CNY                           

9235300  Plunger tip, syringe 2,5ml             CNY                           

9235117  Fuse F4A, v1.00           CNY                           

9022818  EZ1 Advanced XL Flip Cap Tube Rack        CNY                           

9234889  Vacuum manifold, 96 seal             CNY                           

9234862  System liquid container (2l Nalgene)            CNY                           

9239555  PCBA, Laser Driver, Unlimited             CNY                           

9238664  Hinge             CNY                            【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】

9238662  Lid with window           CNY                           

9238659  Cooling compressor T2168GK, 60Hz          CNY                           

9238645  Brake resistor 112 Ohm / 100 W          CNY                           

9242061  Pipetting container, IQ/OQ, QS            CNY                           

9241827  Pyro Q96 ID, Premium Agreement       "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9241166  Board, A/D, QX            CNY                           

9240955  Sensor, z, handler, Qsym              CNY                           

9240835  QIAcube, Basic Agreement, no PM      The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY                           

9240766  32 OUT Module, D2-32TD1, AP96             CNY                           

6000-5018      MST STM Rack & Lid (Blue - 2 pos. cal.)          CNY                           

9241777  QIAxtractor, Inspection Service    "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY                           

9241375  Magnet, door lock, top, EZ1a              CNY                           

9241334  Keypad foil, EZ1           CNY                           

9240750  Belt, Elevator, AP           CNY                           

9243648  Acceler. Valid Supp, Other Extract Sys The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).      CNY                           

QY-x2086       HIH 3T3 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                         

QY-x2087       L929 小鼠成纤维细胞 1640+10%胎牛血清                                       

QY-x2088       HSC-T6 大鼠肝星状细胞 1640+10%胎牛血清                                   

QY-x2089       L-02 人肝细胞 1640+10%胎牛血清                                    

QY-x2090       SRA01/04 人晶体上皮细胞 MEM+非必需氨基酸＋10%胎牛血清                                   

QY-x2091       CHO 中国仓鼠卵巢细胞（野生型） 1640+10%胎牛血清                                        

QY-x2092       IEC-6 大鼠小肠隐窝上皮细胞 DMEM+10%胎牛血清                                      

QY-x2093       P815 小鼠肥大细胞 1640+10%胎牛血清                                    

QY-x2094       NRK-49F 大鼠正常肾成纤维细胞 1640+10%胎牛血清                                    

QY-x2095       自建 正常成人皮肤成纤维细胞 DMEM+20%胎牛血清 原代培养                                         

QY-x2096       HELF 人胚肺成纤维细胞 1640+10%胎牛血清 5，6代                                    

QY-x2097       293 人胚肾细胞 DMEM+10%胎牛血清                                      

QY-x2098       293T 人胚肾T细胞 1640+10%胎牛血清                                    【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】

QY-x2099       GES-1 人胃粘膜细胞 DMEM+10%胎牛血清                                     

QY-x2100       3T3-L1 小鼠胚胎成纤维细胞（前脂肪） DMEM+10%胎牛血清                                    

QY-x2101       Vero 猴肾细胞 1640+10%胎牛血清                                     

QY-x2102       CHO-dhFr- 中国仓鼠卵巢细胞(二氢叶酸缺陷型） 1640+10%胎牛血清                                        

QY-x2103       V79 中国仓鼠肺细胞 1640+10%胎牛血清                                         

QY-x2104       PA317 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                     

QY-x2105       Cos-7 绿猴肾细胞 DMEM+10%胎牛血清                                   

QY-x2106       MA104 非洲绿猴胚胎肾上皮细胞 DMEM+10%胎牛血清                                        

QY-x2107       16HBE 人支气管上皮细胞 1640+10%胎牛血清                                        

QY-x2108       Marc145 猴胚胎肾上皮细胞                                         

QY-x2109       MDA-MB-435S 人乳腺导管癌细胞                                      

QY-x2110       MDBK 瘤牛肾细胞                                        

QY-x2111       MEG-01 人成巨核细胞白血病细胞                                      

QY-x2112       MFC 小鼠胃癌细胞                                       

QY-x2113       MG-63 人骨肉瘤细胞                                    

QY-x2114       MLA144长臂猿淋巴瘤细胞                                         【猪凋亡相关因子(FAS/CD95)ELISA 试剂盒厂家】

QY-x2115       MouseM 小鼠肌肉细胞                                         

QY-x2116       MouseS 小鼠皮肤成纤维样细胞                                   

QY-x2117       Hep G2人肝癌细胞系                                    

QY-x2118       Hep3B人肝癌细胞系