猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供最佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于最高标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为最佳的性能在给定的体积

11。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由最终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的最佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高（或较低），比那些在手册中，与我的试剂盒来了。为什么？

光学密度受影响

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9018570  Guides, door, L/R, EZ1a            CNY                              【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

670213     ipsogen BCR-ABL1 mbcr (BCR) Kit (24)             CNY                             

389004     Investigatot IDproof Client Key                 CNY                             

383615     Investigator Argus Y-12QS Kit (100)                   CNY                             

383617     Investigator Argus Y-12QS Kit (400)                   CNY                             

381315     Investigator Nonaplex ESS Kit (100)                  CNY                             

130915     NeXtal DWBlock ProComplex Suite          CNY                             

130911     NeXtal DWBlock MBClass Suite                 CNY                             

4801064  careHBV PCR ASSAY V2             CNY                             

6000-5025        MST Conversion System            CNY                             

L2133       Expand 4 Pipettor Manual (CD-ROM)               CNY                             

9240708  Filter, Vacuum, 40 Micron, AP196/98                CNY                             

9243656  Post-validation Competency Training       """Post-validation Competency Lab Training includes training of the laboratory DNA staff on QIAGEN instrumentation ａnd chemistry, ａnd a review of information of the validation design plan ａnd results of the validation study. Training will be presented both via seminar ａnd hands-on-learning. Attendees will receive a Certificate of Attendance from QIAGEN to provide documentation of training. As required by FBI regulations, the laboratory is then responsible for the competency testing of all DNA analysts to perform lab work validated. The training presentation ａnd curriculum vitae of the trainer may be provided upon request.

Note:

The Post-validation Competency Training is required for all Comprehensive Validation Study plans but can be included at zero cost.

The Post-validation Competency Training is optional for both Guided ａnd Accelerated Validation Study plans ａnd so charges should apply accordingly.

"""   CNY                             

9022641  therascreen KRAS Assay Package CE                 CNY                             

5050-12200A   PC Country Kit, EU            CNY                             

274603     flocktype Mycoplasma Ms Ab (5)              CNY                             

285215     cador B. hyodysenteriae PCR Reagent (96)              CNY                              【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

273405     pigtype Toxoplasma Ab (20)              CNY                             

273801     pigtype Yersinia Ab (1)               CNY                             

280375     virotype BVDV RT-PCR Kit (96)                  CNY                             

937156     QIAsymphony DSP AXpH DNA Kit (192)            CNY                             

9018702  EZ1 Adv. XL DSP DNA Blood Card              CNY                             

9001907  #QIAgility (w/o PC) PrioPLUS   "QIAgility (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

800337     Transformation                  CNY                             

9019616  ZLV Rail, worktable, plate, Cx2                  CNY                             

9019882  #Lid with LED, blue, RG6          CNY                             

9020585  Spring - Gas, Door Support, DML 3000             CNY                             

9011930  Reagent holder, tubes ａnd bottles  Holder for accommodating 4 microtubes (1.5 ml), 4 tubes (13 ml), ａnd 2 bottles (125 ml ａnd 60 ml) on the worktable   CNY                             

9013456  ZLV Buchsenleiste Autocom 2 pol AWG28                 CNY                             

9022131  Lid, careHPV             CNY                             

9022472  Shipment Box, DML3000                   CNY                             

9237133  #RapidPlate, Full Agreement  "Repair service delivery: 2 business days

Service response time (repair): Yes, 1/agreement year

Periodic inspection/maintenance: Onsite

Inspection/maintenance delivery: Yes, included

Cost coverage for repair parts: Yes, included

Cost coverage for labor: Yes, included

Cost coverage for travel: Yes, included

Replacement system (Loaner) provision: Not included

Transportation cost coverage: Not applicable"       CNY                             

QY-x2416 人胚肺细胞 MRC-5 询价 ATCC                                                

QY-x2417 人胃癌细胞 MGC80-3 询价 ATCC                                                    

QY-x2418 犬肾细胞系 MDCK 询价 ATCC                                                  【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

QY-x2419 牛肾细胞 MDBK (NBL-1) 询价 ATCC                                                

QY-x2420 人乳腺癌细胞 MDA-MB-231 询价 ATCC                                                 

QY-x2421 人乳腺癌细胞 MCF7 询价 ATCC                                                       

QY-x2422 大鼠成肌细胞 L6 询价 ATCC                                                    

QY-x2423 小鼠成纤维细胞 L929 询价 ATCC                                                    

QY-x2424 人T淋巴细胞白血病细胞 Jurkat 询价 ATCC                                                  

QY-x2425 人结肠癌细胞 HT-29 询价 ATCC                                                      

QY-x2426 人纤维肉瘤细胞系 HT-1080 询价 ATCC                                                  

QY-x2427 人卵巢癌细胞 HO-8910                                                     

QY-x2428 人肝正常细胞 HL-7702 询价 ATCC                                                  

QY-x2429 人原髓细胞白血病细胞 HL-60 询价 ATCC                                                      

QY-x2430 肾小管株 HK-2 询价 ATCC                                                        

QY-x2431 小鼠肝癌细胞 Hepa 1-6 询价 ATCC                                                

QY-x2432 人肝癌细胞系 Hep-3B 询价 ATCC                                                   

QY-x2433 人喉表皮癌细胞 HEp-2 询价 ATCC                                                 

QY-x2434 人肝癌细胞 Hep 3B2.1-7 询价 ATCC                                                         【猪乙酰胆碱受体抗体(AChRab)ELISA 试剂盒厂家】

QY-x2435 人宫颈癌细胞 Hela 询价 ATCC                                               

QY-x2436 大鼠心肌细胞 H9c2(2-1) 询价 ATCC                                                        

QY-x2437 人乳腺细胞 HBL-100 询价 ATCC                                                      

QY-x2438 人T淋巴细胞系 H9 询价 ATCC                                                        

QY-x2439 小鼠淋巴瘤细胞 EL-4 询价 ATCC                                                     

QY-x2440 人前列腺癌细胞 DU 145 询价 ATCC                                                        

QY-x2441 人成巨核细胞白血病细胞 Daudi 询价 ATCC                                                  

QY-x2442 非洲绿猴SV40转化的肾细胞 COS-7 询价 ATCC                                                    

QY-x2443 人结肠腺癌细胞 COLO 320 询价 ATCC                                                    

QY-x2444 人鼻咽癌细胞 CNE 询价 ATCC                                                

QY-x2445 人肺腺癌 (胸水) Calu-3 询价 ATCC                                                  

QY-x2446 人急性淋巴细胞白血病T淋巴细胞 CCRF-CEM 询价 ATCC                                                   

QY-x2447 人肾透明细胞癌皮肤转移细胞 Caki-1 询价 ATCC                                                        

QY-x2448 Burkitts淋巴癌细胞系 CA46 询价 ATCC