小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家

 1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

84020       Vacuum Pump (230V, 50Hz)              CNY                              【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】

75842       RNeasy Lipid Tissue Midi Kit (10)     10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), QIAzol Lysis Reagent, RNase-free Reagents ａnd Buffers       CNY                             

75144       RNeasy Midi Kit (50)         50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents ａnd Buffers   CNY                             

28006       MinElute PCR Purification Kit (250) 250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)  CNY                             

73304       Rneasy Microarray Tissue Mini Kit (50)            CNY                             

19585       S-Blocks (24)     "Succsessor of

#19573 Square-Well Blocks (24)"     CNY                             

19579       Flat-Bottom Blocks (24)   96-well blocks with 2 ml wells: 24 per case, lids included         CNY                             

19566       Collection Microtube Caps       Nonsterile polypropylene caps for collection microtubes (1.2 ml) ａnd round-well blocks, 960 in strips of 8 CNY                             

19131       QIAGEN Proteinase K (2) 2 ml (>600 mAU/ml, solution)    CNY                             

19077       Buffer AE 240 ml Elution Buffer for 1000 preps       CNY                             

19056       Buffer QF 1000 ml Elution Buffer     CNY                             

28106       QIAquick PCR Purification Kit (250) For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)      CNY                             

9011498  Cable, Prolongation, BCR X-Motor, 3000mm            CNY                             

9013168  Tubing, syringe outlet, BR8000                 CNY                             

9011413  Plate Turntable for Plato 7                CNY                             

9011411  BGR Greifer PK80 (alt)              CNY                             

9011404  Vacuum pump, control box               CNY                              【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】

9243555  Care HPV, Full Agreement, no PM, 5yrs  "Repair service delivery: send to Regional Repair Center

Service response time (repair): not applicable

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system provision: yes, included

Transportation cost coverage: yes, included" CNY                             

AT-01        #DxS BCR-ABL T315I Mutation Test Kit            CNY                             

9239573  #Warranty PLUS 2, BR Universal     The Warranty PLUS 2, BR Universal extends the warranty period of the BioRobot Universal System to 3 years. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Costs for service visits are fully covered including travel, labor, ａnd parts, plus the cost of kits required for preventive maintenance. With a Warranty PLUS 2, there is a priority response time of 48 hours (2 working days).        CNY                             

676413     ipsogen MPL W515L/K MutaScreen Kit (24)             CNY                             

9011163  Y-Motor for Plato 7           CNY                             

9010970  Plate stacker, Detachable                 CNY                             

1007595  Penta.His Antibody, (1000)                CNY                             

995652     Filter-Tips, 1000µl, M48 (1000)                CNY                             

673813     ipsogen JAK2 MutaSearch Kit (24)            CNY                             

673523     ipsogen JAK2 MutaQuant Kit (24) CE                 CNY                             

337832     qBiomarker SYBR Fluor Mastermix (12)           CNY                             

337820     qBiomarker SYBR ROX Mastermix (2)               CNY                             

9018917  Adapter, 72x0.1ml Strip Tubes                   CNY                             

9018897  Rotor-Disc Pipetting Aid           CNY                             

9018708  EZ1 Adv. XL Virus Card v2.0               CNY                             

QY-x2134 SK-Hep-1 人肝癌细胞系                                                     

QY-x2135 PLC/PRF/5 人原发性肝癌细胞系                                                        

QY-x2136 QGY-7703 人原发性肝癌细胞系                                                        

QY-x2137 SMMC-7721 人肝癌细胞系                                                        

QY-x2138 Huh-7 肝癌细胞系                                                      

QY-x2139 LM-6 肝癌细胞系                                                        

QY-x2140 LM-3 肝癌细胞系                                                        

QY-x2141 LO2人正常肝细胞系                                                  

QY-x2142 SW480 [SW-480]人结肠癌细胞系                                                        

QY-x2143 SW620 [SW-620]人结肠癌细胞系                                                        

QY-x2144 HT-29 人结肠癌细胞系                                              

QY-x2145 LoVo人结肠癌细胞系                                                

QY-x2146 HCT 116 结肠癌细胞系                                                       【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】

QY-x2147 Hep-2人喉癌细胞系                                                   

QY-x2148 HEK-293人胚肾细胞系                                               

QY-x2149 A-549人肺癌细胞系                                                   

QY-x2150 B16小鼠黑色素瘤细胞系                                                  

QY-x2151 Calu-6人肺癌细胞系（未分化癌）                                                  

QY-x2152 CHO-K1中国仓鼠卵巢细胞                                                

QY-x2153 ES-2人卵巢透明细胞癌细胞系                                                 

QY-x2154 H460人大细胞肺癌细胞系                                               

QY-x2155 HeLa 人宫颈癌细胞系                                                        

QY-x2156 HeLa 229人宫颈癌细胞系                                                 

QY-x2157 HL-60人髓性白血病细胞系（早幼粒白血病）                                                        

QY-x2158 Jurkat 人白血病T细胞系                                                  

QY-x2159 K-562人类红白血病细胞系                                                        

QY-x2160 MC3T3-E1小鼠成骨细胞                                                     

QY-x2161 A498人肾癌细胞                                                

QY-x2162 McCoy小鼠成纤维细胞                                                       【小鼠卵白蛋白特异性IgE(OVA-sIgE)ELISA试剂盒厂家】

QY-x2163 MCF-7人乳腺癌细胞系