猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

291115     mericon RR Soy Kit (96)            CNY                              【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】

291065     mericon GMO Screen bar (96)                   CNY                             

9001933  DML 3000 120-240V Q              CNY                             

9001915  PyroMark Q96 ID, Priority        PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133120     NeXtalStock Tricine (200)                   CNY                             

133180     NeXtalStock Citric acid pH 3.0 (200)                  CNY                             

9018503  Manual air supply, service tool, QX           CNY                             

51161       QIAamp 96 DNA Blood Kit (4) For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                             

133106     NeXtalStock Sodium ac. H2O pH 4.6 (200)                CNY                             

9015519  Spring, for door                  CNY                             

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority  QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.  CNY                             

9001875  EZ1 Advanced XL, Priority        EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

959236     MagAttract RNA Tissue Mini M48 Kit (192)             CNY                             

956336     MagAttract RNA Univ. Tissue M48 Kit(192)             CNY                             

9018457  Cartridge, alignment tool, QX          CNY                             

9015467  Motor, P            CNY                             

9015451  Modules, DISU, BR8000 v2.1            CNY                              【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】

9012427  Thermostate system, complete, 120V, BR30           CNY                             

9018483  Notebook, QX           CNY                             

51297       FlexiGene DNA AGF3000 Kit (640)  "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"    CNY                             

51185       QIAamp DNA Blood Midi Kit (100)  For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)   CNY                             

38075       AIL Cartridge (5ml)           CNY                             

9015471  Sensor, photo, PM-U24             CNY                             

956034     EZ1 RNA Universal Tissue Kit (48)   Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.         CNY                             

204772     Rotor-Gene Multiplex PCR Kit (80)  The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.       CNY                             

9018435  Tubing, external N2 source, 10ft, QX                CNY                             

9018417  Cover, front, blue window, QX          CNY                             

9018403  ZLV, Detect barrel (1), QX                  CNY                             

290103     mericon Shigella spp Kit (24)             CNY                             

9015392  Clamp, rubber, adapter plate, HS shaker,                 CNY                             

9001793  QIAcube HT/QIAxtractor, system              CNY                             

9012330  Syringe Pump outlet tubing               CNY                             

QKT0965  尿道炎荧光检测试剂盒                                                       

QKT0966  淋病荧光检测试剂盒                                                  

QKT0967  乙型肝炎病毒核心IgM抗体检测试剂盒                                                  

EK0301     hActA-ELISA     人活化素A酶联免疫试剂盒         Human Activin A ELISA Kit       96 tests                      

EK0305     hANG-ELISA     人双调蛋白酶联免疫试剂盒         Human ANG ELISA Kit      96 tests                      

EK0307     hBDNF-ELISA   人脑源性神经生长因子酶联免疫试剂盒     Human BDNF ELISA Kit    96 tests                      

EK0310     hBMP-5-ELISA 人BMP-5酶联免疫试剂盒    Human BMP-5 ELISA Kit  96 tests                      

EK0311     hBMP-2-ELISA 人BMP-2酶联免疫试剂盒    Human BMP-2 ELISA Kit  96 tests                       【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】

EK0314     hBMP-4-ELISA 人BMP-4酶联免疫试剂盒    Human BMP-4 ELISA Kit  96 tests                      

EK0325     hEGF-ELISA       人表皮生长因子酶联免疫试剂盒         Human EGF ELISA Kit       96 tests                      

EK0327     hEGFR-ELISA    人表皮生长因子受体酶联免疫试剂盒         Human EGFR ELISA Kit    96 tests                      

EK0335     hFAS-ELISA        人凋亡相关因子酶联免疫试剂盒         Human FAS ELISA Kit        96 tests                      

EK0337     hFASL-ELISA      人凋亡相关因子配体酶联免疫试剂盒         Human FASL ELISA Kit      96 tests                      

EK0348     hFGF-9-ELISA   人FGF-9酶联免疫酶联免疫试剂盒      Human FGF-9 ELISA Kit    96 tests                      

EK0349     hFN-ELISA         人纤维连接蛋白酶联免疫试剂盒         Human Fibronectin ELISA Kit  96 tests                      

EK0360     hG-CSF-ELISA   "人粒细胞集落刺激因子酶联免疫试剂盒

"       Human G-CSF ELISA Kit    96 tests                      

EK0362     hGDNF-ELISA   人神经胶质细胞源性的神经营养因子酶联免疫试剂盒     Human GDNF ELISA Kit   96 tests                      

EK0364     hGM-CSF-ELISA         "人粒细胞巨噬细胞刺激因子酶联免疫试剂盒

"       Human GM-CSF ELISA Kit         96 tests                      

EK0369     hHGF-ELISA      人肝细胞生长因子酶联免疫试剂盒     Human HGF ELISA Kit      96 tests                      

EK0370     hICAM-1-ELISA         人细胞间粘附分子-1酶联免疫试剂盒         Human ICAM-1 ELISA Kit          96 tests                      

EK0373     hIFN-γ-ELISA  人IFN-γ酶联免疫试剂盒     Human IFN-γ ELISA Kit 96 tests                      

EK0376     hIGF-1-ELISA    人IGF-1酶联免疫试剂盒       Human IGF-1 ELISA Kit    96 tests                      

EK0379     hIGF-2-ELISA    人IGF-2酶联免疫试剂盒       Human IGF-2 ELISA Kit    96 tests                      

EK0382     hIGFBP-ELISA   人IGFBP-1酶联免疫试剂盒  Human IGFBP-1 ELISA Kit         96 tests                       【猪脑钠素/脑钠尿肽(BNP)ELISA 试剂盒现货】

EK0386     hIGFBP-3-ELISA        人IGFBP-3酶联免疫试剂盒  Human IGFBP-3 ELISA Kit         96 tests                      

EK0389     hIL-1a-ELISA     人IL-1α酶联免疫试剂盒      Human IL-1α ELISA Kit 96 tests                      

EK0392     hIL-1α-ELISA  人IL-1α酶联免疫试剂盒      Human IL-1α ELISA Kit 96 tests                      

EK0397     hIL-2-ELISA       人 IL-2酶联免疫试剂盒        Human IL-2 ELISA Kit       96 tests                      

EK0402     h IL-3-ELISA      人IL-3 酶联免疫试剂盒        Human IL-3 ELISA Kit       96 tests                      

EK0404     h IL-4-ELISA      人 IL-4酶联免疫试剂盒        Human IL-4 ELISA Kit       96 tests                      

EK0407     h IL-5-ELISA      人 IL-5酶联免疫试剂盒        Human IL-5 ELISA Kit       96 tests                      

EK0410     hIL-6-ELISA       人 IL-6酶联免疫试剂盒        Human IL-6 ELISA Kit       96 tests                      

EK0413     hIL-8-ELISA       人 IL-8酶联免疫试剂盒        Human IL-8 ELISA Kit       96 tests