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本试剂盒仅供研究使用,齐一生物销售:021-6034 8496;181214 53965;173021 04490欢迎选购!
操作步骤
1.准备试剂,样品和标准品
2.加入准备好的样品和标准品,37℃反应30分钟
3.洗板5次,加入酶标试剂,37℃反应30分钟
4.洗板5次,加入显色液A、B,37℃显色15分钟
5.加入终止液
6.15分钟之内读OD值
7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。
【小鼠c-junELISA试剂盒价格】样本保存条件:
1.2-8℃冰箱最多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
1.血清样本用干燥管(红色)或者促凝管(黄色)收集
2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
3.动物组织匀浆(细胞):
1g组织加入9ml PBS(浓度为0.1M,pH为7.4的)测分泌型指标
1g组织加入1ml组织裂解液加入8ml PBS 测细胞内
粪便样本:1g样本+9ml PBS(浓度为0.01M,pH为7.4的)
没有PBS的话也可以用一下生理盐水,最好还是用PBS
【小鼠c-junELISA试剂盒价格】样本离心转速及时间
1.试管采集的样本:3000转,离心五分钟取上清
2.离心管或者EP管采集的样本:5000--8000转3分钟取上清
3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
【小鼠c-junELISA试剂盒价格】以上样本采集及处理方式供您参考,如有问题请来电,齐一生物销售:021-6034 8496;181214 53965;173021 04490
9012736 Peristaltic pump outlet tubing (1.0 m) CNY 【小鼠c-junELISA试剂盒价格】
9012725 Syringe pump outlet for Plato CNY
979001 PyroMark Q96 Plate (100) "For sample analysis of DNA template prepared with magnetic beads for ID.
Magnetic bead technology instead of vacuum prep tool is used here" CNY
9012706 Base, auto vac, BR8000 This is the robovac base for the BR8000 I. There are 5 contacts. CNY
133240 NeXtalStock Sodium hydroxide (200) CNY
133233 NeXtalStock Sodium cacodyl. pH 7.5 (100) CNY
9002195 RGQ 2plex, PC, PrioPLUS, basic, no PM "RGQ 2plex, PC Instrument
Installation of RGQ 2plex & Introductory training
Warranty extension to 3 yrs
Repair Center service, response 7-10 days
No Loaner available
No preventive Maintenance
Priority service provision over non-contract customers
Documented installation & repair report
Unlimited number of repair service
All cost covered (Shipping, labor, parts)" CNY
9002039 Rotor-Gene Q MDx 5plex HRM, PrioPLUS "Rotor-Gene Instrument
Installation of Rotor-Gene & Introductory training
Warranty extension to 3 yrs
Regional Repair Center service, 7-10 day turn around time
Loaner available in 2 days*
Annual Inspections (1 PM per yearr)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (shipping, labor, parts)" CNY
59436 EpiTect MethyLight PCR Kit (200) CNY
971540 therascreen® UGT1A1 Pyro Kit (24) CNY
133217 NeXtalStock MOPS pH 7.5 (200) CNY
51504 QIAamp DNA Stool Mini Kit (50) For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml) CNY
9002013 Rotor-Gene Q MDx 2plex HRM System CNY
970032 PyroMark Q24 CpG MGMT (4 x 24) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene. CNY
922920 LiquiChip Filter Microplates (10) CNY
292023 mericon Cattle (24) CNY
292013 mericon Pig Kit (24) CNY 【小鼠c-junELISA试剂盒价格】
9001903 QIAgility HEPA/UV (PC) Priority QIAgility HEPA/UV (PC) Priority includes the QIAgility instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor and travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents. CNY
133122 NeXtalStock Potassium citrate (200) CNY
963141 QIAquick 96 PCR BioRobot Kit (4) For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads CNY
133193 NeXtalStock di-Potassium phosphate (200) CNY
9018555 Cover, front, top, EZ1a CNY
9018528 User Manual EZ1 Advanced CNY
37601 Qproteome Plasma Membrane Protein Kit CNY
9015329 O-rings, Norpren tube fixing, M48 (2) CNY
9015328 Seal, cap, M48 (6) CNY
239095 GelPilot 1 kb Plus Ladder (100) Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp CNY
133127 NeXtalStock Sodium citrate (200) CNY
133126 NeXtalStock TRIS-HCl pH 8.5 (200) CNY
133008 NeXtalStock 2-Propanol (200) CNY
133007 NeXtalStock MES sod. salt untit. (200) CNY
9011949 Board, X-main CNY
EK0445 mMCSF-ELISA 小鼠巨噬细胞集落刺激因子ELISA试剂盒 Mouse M-CSF ELISA Kit 96 tests
EK0460 mMMP-2-ELISA 小鼠MMP-2ELISA试剂盒 Mouse MMP-2 ELISA Kit 96 tests
EK0462 mMMP3-ELISA 小鼠MMP-3ELISA试剂盒 Mouse MMP-3 ELISA Kit 96 tests
EK0466 mMMP-9-ELISA 小鼠MMP-9ELISA试剂盒 Mouse MMP-9 ELISA Kit 96 tests
EK0470 mNGF-ELISA 小鼠NGF/NGFβELISA试剂盒 Mouse NGF/NGFβ ELISA Kit 96 tests
EK0473 mNT3-ELISA 小鼠Neurotrophin-3 ELISA试剂盒 Mouse Neurotrophin-3 ELISA Kit 96 tests
EK0483 mOPN-ELISA 小鼠骨桥素Elisa试剂盒ELISA试剂盒 Mouse OPN ELISA Kit 96 tests 【小鼠c-junELISA试剂盒价格】
EK0486 mPDGF-AB-ELISA 小鼠血小板衍生生长因子ABELISA试剂盒 Mouse PDGF-AB ELISA Kit 96 tests
EK0495 mRantes-ELISA 小鼠趋化因子ELISA试剂盒 Mouse Rantes ELISA Kit 96 tests
EK0504 mSELL-ELISA 小鼠L选择素Elisa试剂盒 Mouse L-Selectin ELISA Kit 96 tests
EK0506 mSELP-ELISA 小鼠P选择素Elisa试剂盒 Mouse P-Selectin ELISA Kit 96 tests
EK0515 mTGF 小鼠转化生长因子β1ELISA试剂盒 Mouse TGFβ1 ELISA Kit 96 tests
EK0527 mTNF-ELISA 小鼠TNFαELISA试剂盒 Mouse TNFα ELISA Kit 96 tests
EK0538 mVCAM-ELISA 小鼠血管细胞粘附分子-1ELISA试剂盒 Mouse VCAM-1 ELISA Kit 96 tests
EK0541 mVEGF-ELISA 小鼠血管内皮生长因子ELISA试剂盒 Mouse VEGF ELISA Kit 96 tests
EK0562 mECad-ELISA 小鼠钙粘着蛋白ELISA试剂盒 Mouse E-Cadherin ELISA Kit 96 tests
EK0577 mGp130-ELISA 小鼠Gp130/IL6STELISA试剂盒 Mouse Gp130/IL6ST ELISA Kit 96 tests
EK0582 mRes-ELISA 小鼠抵抗素ELISA试剂盒 Mouse Resistin ELISA Kit 96 tests
EK0664 mBAFF-ELISA 小鼠BAFFELISA试剂盒 Mouse BAFF ELISA Kit 96 tests
EK0668 mPCad-ELISA 小鼠P-CadherinELISA试剂盒 Mouse P-Cadherin ELISA Kit 96 tests
EK0703 mCD40-ELISA 小鼠CD40/TNFRSF5ELISA试剂盒 Mouse CD40/TNFRSF5 ELISA Kit 96 tests
EK0708 mCD80-ELISA 小鼠B7-1/CD80ELISA试剂盒 Mouse B7-1/CD80 ELISA Kit 96 tests
EK0717 mCTLA4-ELISA 小鼠CTLA4ELISA试剂盒 Mouse CTLA4 ELISA Kit 96 tests 【小鼠c-junELISA试剂盒价格】
EK0780 mIL7-ELISA 小鼠IL-7 ELISA试剂盒 Mouse IL-7 ELISA Kit 96 tests
EK0808 mCSF1R-ELISA 小鼠CSF1R/M-CSFRELISA试剂盒 Mouse CSF1R/M-CSFR ELISA Kit 96 tests
EK0830 mRANK-ELISA 小鼠Receptor Activator of NF-κB (RANK)ELISA试剂盒 Mouse Receptor Activator of NF-κB (RANK) ELISA Kit 96 tests
EK0866 mIL23-ELISA 小鼠IL-23ELISA试剂盒 Mouse IL-23 ELISA Kit 96 tests
EK0874 mPECAM-ELISA 小鼠 PECAM-1/CD31ELISA试剂盒 Mouse PECAM-1/CD31 ELISA Kit 96 tests
EK0925 mDKK-1-ELISA 小鼠DKK1ELISA试剂盒 Mouse DKK1 ELISA Kit 96 tests
EK0308 rBDNF-ELISA 大鼠脑源性神经生长因子ELISA试剂盒 Rat BDNF ELISA Kit 96 tests
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