小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家

规格:小规格48T.大规格96T

样本标准：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：酶联免疫分析（ELISA）

运输保存：2~8°贮存.保质期：6个月

试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】齐一生物主要经营进口原装Elisa试剂盒,检测试剂种类齐全,价格实惠！购买齐一生物产品的新老客户都可享免费代测实验服务,全程提供技术支持.如需其它Elisa试剂盒说明书及产品价格,可来电咨询齐一生物销售：021-6034 8496；181214 53965；173021 04490

试剂盒特点

一、高效、灵敏、特异的抗体；

二、稳定的重复性和可靠性；

三、吸附性能好,空白值低,孔底透明度高的固相载体；

四、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型；

试剂盒可概括四个方面：

1、免疫酶染色各种细胞内成份的定位.

2、研究抗酶抗体的合成.

3、显现微量的免疫沉淀反应.

4、定量检测体液中抗原或抗体成份.

【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】ELISA试剂盒为您节省实验经费.

elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度.比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%.

【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】试剂盒制备方法包括以下步骤：

1)抗原表位的计算机筛选；

2)抗原的制备；

3)血清的采集；

4)间接ELISA方法的建立；

5)间接ELISA方法的敏感性和特异性验证；

6)试剂盒的稳定性验证；

7)对大量样本进行检测.该方法简单、快速、灵敏度高、特异性强、稳定性合格、重复性好.应用本发明制得的试剂盒能有效检出样本含量.适用于大批量样本的检测

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

L2170     hc2 CT/GC HC2 DNA Test PI (CD-ROM)          CNY                            【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】

L1971     Rotary Shaker User＇s Manual        CNY                           

9240705  O-ring Kit, Capper, Spindle, AP            CNY                           

9240693  Actril Sterilant & Test Strips, AP          CNY                           

9243653  Comprehen Valid., Other Extraction Sys       """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """   CNY                           

386516    Investigator STR GO! Lysis Buffer (200)            CNY                           

208256    QuantiNova Probe PCR Kit (2500)              CNY                           

208254    QuantiNova Probe PCR Kit (500)         CNY                           

180984    GeneRead Adapter I Set 12plex (72)            CNY                           

614035    careHPV Test, SFDA (96-test)             CNY                           

282605    virotype Influenza A RT-PCR Kit (96)          CNY                           

272703    pigtype PRRSV Ab (5)          CNY                           

273003    pigtype Salmonella Ab (5)             CNY                           

280377    virotype BVDV RT-PCR Kit (480)        CNY                           

9018462  Marker Kit, QC tool, QX              CNY                           

800372    Shipping, Postage, Packaging (express)              CNY                           

800373    Data Carrier           CNY                            【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】

9019521  ZLV ADAPTER KIT M&N 8 STRIP           CNY                           

9019595  ZLV Bush, tip stripper, Cx8          CNY                           

9019935  Cable harness, X-axis 8 core, CQX             CNY                           

9020596  Tape Foam,045X750 Double Stick,DML 3000            CNY                           

9017843  Tool, cable, communication, RS232, QIAC         CNY                           

9013401  Cable set, cooling system, 90/120        CNY                           

9234873  ZLVPipetting Station for 2 microtit.             CNY                           

9013395  Tip disposal bags           CNY                           

9013391  Tubing set, PVC, BR9604            CNY                           

9013268  Cable, card-X to solenoid valve, 24V           CNY                           

9013222  Holder, tube, STS, single, 11 ml (30), 0              CNY                           

9013204  Transportation kit, BR8000           CNY                           

9011759  Reagent holder, 4-tube 13ml  Holder for accommodating 4 microcentrifuge tubes (13 ml) on the worktable; must be used with the reagent holder tray   CNY                           

9011672  Reader Abdeckung spez. GSG (9194)          CNY                           

132605    NeXtal Stock Sodium ac. H2O (50)      1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)  CNY                           

QY-G054 人涎腺腺样囊性癌细胞       Acc-3     上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G055 人鼻咽癌母系细胞       CNE-2Z  上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G056 人喉表皮癌细胞    Hep-2      上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G057 人口腔上皮癌细胞       KB   上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G058 人咽鳞癌细胞       FaDu      上皮细胞       贴壁       MEM      胎牛血清      

QY-G059 人舌癌细胞    Tca-8113 上皮细胞       贴壁       RPMI-1640    胎牛血清      

QY-G060 人甲状腺导管癌细胞    TT        上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G061 人甲状腺鳞癌细胞       SW579   上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G062 人肺扁平上皮癌细胞    QG-56    上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G063 人肺腺癌细胞       A549      上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G064 人肺腺癌细胞       SPC-A-1 上皮细胞       贴壁       DMEM(high glucose)    小牛血清      

QY-G065 人肺腺癌细胞       LTPEP-a-2     上皮细胞       贴壁       RPMI-1640    胎牛血清      

QY-G066 人肺腺癌细胞       H1299     上皮细胞       贴壁       RPMI-1640    胎牛血清       【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】

QY-G067 人高转移肺癌细胞       95-D       上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G068 人肺癌细胞    H125     上皮细胞       贴壁       RPMI-1640    胎牛血清      

QY-G069 人肺癌细胞    NCI-H460     上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G070 人小细胞肺癌细胞       NCI-H446     上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G071 人大细胞肺癌细胞       NCI-H661      上皮细胞       贴壁       RPMI-1640    胎牛血清      

QY-G072 人巨细胞性肺癌细胞    801-D     上皮细胞       贴壁       RPMI-1640    胎牛血清      

QY-G074 人肺癌细胞（淋巴结转移）       NCI-H292      上皮细胞       贴壁       RPMI-1640    胎牛血清      

QY-G075 人肺鳞癌细胞       SK-MES-1      上皮细胞       贴壁       DMEM(high glucose)    胎牛血清      

QY-G076 人肺腺癌细胞（胸膜渗出液）    Calu-3     上皮细胞       贴壁       DMEM(high glucose)    小牛血清      

QY-G077 人胸膜瘤细胞       SMC-1    上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G078 人结肠癌细胞       LOVO     上皮细胞       贴壁       DMEM(high glucose)    小牛血清      

QY-G079 人结肠癌细胞      HCT-8    上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G080 人结肠癌细胞       HCT-116 上皮细胞       贴壁       McCoy’s 5A 小牛血清      

QY-G081 人结肠癌细胞       HT-29     上皮细胞       贴壁       DMEM(high glucose)    小牛血清      

QY-G082 人结肠癌细胞       COLO205       上皮细胞       贴壁       RPMI-1640    小牛血清      

QY-G083 人结肠癌细胞       SW480    上皮细胞       贴壁       DMEM(high glucose)    小牛血清       【小鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒厂家】

QY-G084 人结肠癌细胞       SW620    上皮细胞       贴壁       RPMI-1640    小牛血清