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小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格

小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格

  • 价 格: 电议
  • 型号:
  • 生 产 地:中国大陆
  • 访问:12次
  • 发布日期:2016/8/3(更新日期:2016/8/3)

齐一生物科技(上海)有限公司

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小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格 QY-M30252 国产/进口原装 48T/96T 齐一生物科技(上海)有限公司销售:021-6034 8496;181214 53965;173021 04490
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 小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格ELISA试剂盒常遇见问题及解答

齐一生物销售:0216034 8496181214 53965173021 04490

1。我必须运行所有的标准和样品的重复?

是的,重复的运行,以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗?

不,每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果,得到与分析?

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化,移液和洗涤技术,培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示?

在不推荐使用条件下的部件组件的存储,以保证测试的正确性能。

5。我应该如何储存我的样品?

样品应保存在-20°C或更低的温度。长期储存,建议冻结他们在- 70余。

6。我可以修改协议吗?

BGELISA试剂盒进行了优化,提供最佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗?

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值,在动态范围内的测定。我可以使用这些值吗?

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的,这可能导致不正确的外推值。产生值高于最高标准的样品应为(进一步)稀释和重复。如果样品低于该测定的范围内,该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗?

是的,这些都是必需的计算,并反映任何微妙但显着的性能变化,从一天到一天,检测到检测。当排除特定的检测问题的来源时,它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗?

不建议你改变卷由于BG工具被设计为最佳的性能在给定的体积

11。可以使用不同的组件组件吗?

每一个组件包含有特定批号的组件,以确保所有的组件都进行优化,以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好,但我没有得到一个信号,我的样本,当我预期,为什么?

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释,请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子?

如果您使用的是一个自动平板洗衣机,我们建议定期检查校准,并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心,以确保所有的内容都是送气和板拍干的无尘纸。小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格

14。我需要用一个盘子吗?

可靠的结果可以得到无板振动,但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间?

对于酶联免疫吸附试验,在双波长读的是做正确的光密度贡献的塑料以及,灯和光学波动。

16。如果我提我的样本,我还需要遵循推荐的稀释液试剂盒中插入了吗?

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由最终用户决定。

17。什么是预期的分析物浓度,我应该找到?

一个给定的分析物的量可能会有所不同,不仅从物种的物种,但也组织和细胞来源。这个信息的最佳来源是目前的文献,很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高(或较低),比那些在手册中,与我的试剂盒来了。为什么?

光学密度受影响

 

 

1.    Do I have to run all of my standards and samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision and increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate and wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted and the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, and reflect any subtle but significant performance changes from day to day and assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didnt get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, and that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated and the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.'s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp and optical fluctuations. 小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification and concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue and cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time and temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir and make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times and temperatures. However, if all wells are intensely and equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9230054  Clamp, Tubing, Z-bar                 CNY                              【小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格】

9234861  Lid coupling piece for system liquid          CNY                             

9239551  Cbl Assy, XY, LED Driver             CNY                             

9238663  Hook, motorized lid lock           CNY                             

9238642  Capacitor board                 CNY                             

9238626  Opto-Encoder           CNY                             

9238625  Eprom and controller 4K15, Zent3C                  CNY                             

9241828  Pyro Q96 MDa, Premium Agreement      "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241122  Superlube                   CNY                             

9241007  QIArent&go - Rent out fee QIAcube                 CNY                             

9240985  PCB, drive, cooling device, Qsym              CNY                             

9240982  Sensor, x, sample tracking, Qsym              CNY                             

9240951  Support, cable, portal, Qsym            CNY                             

9234820  Manual, user, QS 4.0, Int                   CNY                             

9240768  Signal Conditioner, Centrifuge, AP96                 CNY                             

9240764  DC Output Module 8NPT, 3AMP, AP96             CNY                             

6000-5003        Duraseal Sealing Film                CNY                              【小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格】

9241781  RGQ, Full Agreement, no PM  "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included" CNY                             

9241776  QIAgility, Inspection Service    "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material and certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected and tested and a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                             

9241756  IQ/OQ Services, QIAsymphony         Instrument qualification support service for the QIAsymphony. The product contains the Installation Qualification (IQ)/Operation Qualification (OQ) documentation and the performance of the qualification protocols (travel and labor costs included).         CNY                             

9237126  Upgrade App., PCR Setup                  CNY                             

9237093  Training, Extension day    Additional days of training may be purchased during the week of the site visit upon request.         CNY                             

9236426  Board, arm interconnect, TWI                  CNY                             

9236421  Switch, firmware loading, TWI                  CNY                             

9236359  Sheath Filter with Quick Disconnect                  CNY                             

9236346  1.5A Fuse LiquiChip           CNY                             

9236338  Quick disconnect Set                  CNY                             

9236164  Syringe block, repl. kit, RapidPlate           CNY                             

9236153  Locator Plate, Tips, pos 2, BR RP              CNY                             

9235698  Cable 24V, RCS1                 CNY                             

9235296  Capillary, Dispenser head, Mounting Tool                 CNY                             

9235054  Incubator Tower (65°C) Board                   CNY                             

QY-L1011 芝麻酚533-31-3      Sesamol   533-31-3  20mg                          

QY-L1012 芝麻林素526-07-8 Sesamolin        526-07-8  20mg                          

QY-L1013 梣酮28808-62-0      Fraxinellone     28808-62-0       20mg                          

QY-L1014 植酸(UV98%83-86-3         Phytic acid        83-86-3    20mg                          

QY-L1015 竹节参皂苷IVa51415-02-2    Chikusetsu saponin a  51415-02-2       20mg                          

QY-L1016 竹节香附素A89412-79-3        Raddeanin(Anemodeanin) A    89412-79-3       20mg                          

QY-L1017 左旋多巴 59-92-7  Levodopa 59-92-7    20mg                          

QY-L1018 棕榈酸1957/10/3     Palmitic acid   21096       20mg                          

QY-L1019 紫檀芪537-42-8   Pterostilbene   537-42-8  20mg                           【小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格】

QY-L1020 紫茎女贞苷A95%cas147396-01-8 Ligupurpuroside A    "147396-01-8

"       20mg                          

QY-L1021 紫茎女贞苷Bcas147396-02-9         Ligupurpuroside B    "147396-02-9

 "     20mg                          

QY-L1022 紫茎女贞苷C95%   Ligupurpuroside C             20mg                          

QY-L1023 紫茎女贞苷D 95%         Ligupurpuroside D             20mg                          

QY-L1024 cis-紫茎女贞苷B 95%   cis- Ligupurpuroside B               20mg                          

QY-L1025 苍术素醇                            20mg                          

QY-L1026 骨化二醇63283-36-3      Calcifediol         63283-36-3       20mg                          

QY-L1027 骨化三醇19356-17-3      Calcifediol         19356-17-3       20mg                          

QY-L1028 雷帕霉素53123-88-9      Sirolimus  53123-88-9       20mg                          

QY-L1029 他克莫司104987-11-3   Tacrolimus        104987-11-3    20mg                          

QY-L1030 硫秋水仙苷602-41-5      Acetamide     602-41-5  20mg                          

QY-L3001 左旋肉碱 541-15-1       L(-)-Carnitine 541-15-1         20mg                          

QY-L3002 正丁胺109-73-9   Butylamine    109-73-9         20mg                          

QY-L3003 丙氨酸 338-69-2  D-Alanine       338-69-2         20mg                          

QY-L3004 L-苯丙氨酸63-91-2         L-Phenylalanine        63-91-2    20mg                          

QY-L3005 达卡巴嗪4342/3/4   Dacarbazine    891986     20mg                           【小鼠骨形成蛋白6(BMP-6)ELISA试剂盒价格】

QY-L3006 蛋氨酸63-68-3        L-Methionine   63-68-3    20mg                          

QY-L3007 L-蛋氨酸63-68-3     L-Methionine   63-68-3    20mg                          

QY-L3008 腐胺 110-60-1       1,4-Diaminobutane        110-60-1         20mg                          

QY-L3009 L-谷氨酸56-86-0     L-Glutamic acid        56-86-0    20mg                          

QY-L3010 甘氨酸56-40-6        Glycine     56-40-6    20mg                           


科研之路,您我并肩同行!齐一生物具有完善的ELISA试剂盒开发平台,成熟的抗原、抗体研发系统,熟练掌握各种酶联技术,如双抗夹心法、(直接)竞争法、间接竞争法、阻断法、间接法、双抗原夹心法等方法,结合我公司的诊断试剂开发团队我们可以有效的将试剂盒开发为临床诊断级别,质量处于全国前茅。齐一生物针对中高端客户推出自主品牌QIYBO试剂盒为了保证客户用货质量,我公司采用纯进口原料现包被生产,质量稳定高.
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