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本试剂盒仅供研究使用,齐一生物销售:021-6034 8496;181214 53965;173021 04490欢迎选购!
操作步骤
1.准备试剂,样品和标准品
2.加入准备好的样品和标准品,37℃反应30分钟
3.洗板5次,加入酶标试剂,37℃反应30分钟
4.洗板5次,加入显色液A、B,37℃显色15分钟
5.加入终止液
6.15分钟之内读OD值
7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。
【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】样本保存条件:
1.2-8℃冰箱最多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】样本收集
1.血清样本用干燥管(红色)或者促凝管(黄色)收集
2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
3.动物组织匀浆(细胞):
1g组织加入9ml PBS(浓度为0.1M,pH为7.4的)测分泌型指标
1g组织加入1ml组织裂解液加入8ml PBS 测细胞内
粪便样本:1g样本+9ml PBS(浓度为0.01M,pH为7.4的)
没有PBS的话也可以用一下生理盐水,最好还是用PBS
【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】样本离心转速及时间
1.试管采集的样本:3000转,离心五分钟取上清
2.离心管或者EP管采集的样本:5000--8000转3分钟取上清
3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】以上样本采集及处理方式供您参考,如有问题请来电,齐一生物销售:021-6034 8496;181214 53965;173021 04490
9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.
All required testing material and certified equipment is provided by the service specialist.
During the half day-long procedure, all application-critical modules of the QIAextractor are inspected and tested and a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY 【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】
9241375 Magnet, door lock, top, EZ1a CNY
9241334 Keypad foil, EZ1 CNY
9240750 Belt, Elevator, AP CNY
9243648 Acceler. Valid Supp, Other Extract Sys The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel and labor charges for a site visit up to 5 days by a QIAGEN Specialist. A QIAGEN specialist will provide provide an introduction to the instrument and hands-on user support. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, and QIAGEN sponsored data templates. The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR and CE setup, normalization, etc. to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation and report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design and are not included by QIAGEN. Attendees will receive a certificate of participation in the hands-on exercises. Please indicate which protocols to validate (Biosprint 96, DNA mericon). CNY
9243553 #BR M96, PM Service "
Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9242864 QIArent&go -Rent out fee QSSP/AS Cabinet CNY
5000-1321 Feet Set/4, SKR1 CNY
5000-1288 DML 2000 - Fuse 160 mA PKG 10 CNY
5000-1287 DML 2000 - Fuse 315 mA PKG 10 CNY
5000-1505 Switch ON-OFF 120V, HTR1 CNY
6000-00181 Plate Washer - Replacement Tubing Kit CNY
6000-00178 Plate Washer - Maintenance Kit CNY
5100-00047 Wash Buffer Concentrate (2 Bottles) "2 bottles of 100mL each.
The price is proportional to 2x 5100-0795 (discontinued)" CNY
9240740 Tubing, Tygon, 1/4" ID CNY
9240735 Tubing, Black, Capper, AP196/98 CNY
9240724 Tube Assy, Draw, AP96/196 CNY 【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】
5000-1412 Poteneiometer 500K, VTX2 CNY
9240506 Monitor, 17", Flat Panel, AP CNY
9239851 Cover, LC200 CNY
9239565 xMAP Alignment Microspheres CNY
9019942 Barrel removal tool, CX1 CNY
9019474 Lid, UV, polycarb, C1x CNY
9238884 Stepper motor, turntable, BS96 CNY
9238866 BR MDx, Premium Agreement The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY
9238857 Basic Cover Agreement Plato3, 1yr The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9238838 #Twister I, Basic Agreement, no PM The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, and parts. With a Basic Cover Agreement, the response time is 5 working days. CNY
9241220 QIAxcel, Installation "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.
This installation covers the handling of the QIAxcel systems hardware and familiarize with the basic instrument and software functions of the BioCalculator software. All travel and labor costs and well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements." CNY
9241195 Cable, RS232, Belkin F2N209-06-T, 1.8m CNY
9241186 Sensor, interlock2, with cable, QX CNY
9238831 BR 8000, Basic Agreement The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9238822 Rect. carrier for 1 tube 100 ml CNY
MADM-b 间充质干细胞-脂肪细胞分化培养基-基础 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal 500 ml
MADM-b-prf 间充质干细胞-脂肪细胞分化培养基-无酚红 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free 500ml
MCDM 间充质干细胞-软骨分化培养基 Mesenchymal Stem Cell Chondrogenic Differentiation Medium 500ml
Ared 茜素红S染色试剂盒 Alizarin Red S Staining Kit 100 ml
Ored 油红O染色试剂盒 Oil Red O Staining Kit 100 ml
PCR 人间质干细胞成脂肪细胞检测试剂盒 Human Mesenchymal Stem Cell Adipogenesis Detection Kit 50 reactions
HMSC-C PCR 人间充质干细胞软骨形成检测试剂盒 Human Mesenchymal Stem Cell Chondrogenesis Detection Kit 50 reactions
SafraninO 番红O染色试剂盒 Safranin O Staining Kit 100 reactions
ABlue 阿利新蓝染色试剂盒 Alcian Blue Staining Kit 100 reactions 【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】
HDF-f 人真皮成纤维细胞--胎儿 Human Dermal Fibroblasts-fetal 5 x 10^5 cells/vial
HDF-n 人真皮成纤维细胞--新生 Human Dermal Fibroblasts-neonate 5 x 10^5 cells/vial
HDF-a 人真皮成纤维细胞--成人 Human Dermal Fibroblasts-adult 5 x 10^5 cells/vial
HEK-n 人表皮角质细胞--新生 Human Epidermal Keratinocytes-neonatal 5 x 10^5 cells/vial
AEpiCM 肺泡上皮细胞培养基 Alveolar Epithelial Cell Medium 500 ml
KM 角质细胞培养基 Keratinocyte Medium 500 ml
HGL PCR 人胚层检测试剂盒 Human Germ Layer Detection Kit 50 reactions
HSC PCR 人干细胞多能性检测试剂盒 Human Stem Cell Pluripotency Detection Kit 50 reactions
ALP 碱性磷酸酶活性测定试剂盒 Alkaline Phosphatase Activity Assay 500 tests
ALPr 碱性磷酸酶染色检测(红色) Alkaline Phosphatase Staining Assay (Red) 500 tests
EndoFectagen® 内皮细胞转染试剂盒 Endothelial Cell Transfection Kit 250 Transfections
FibroFectagen® 成纤维细胞转染试剂 Fibroblast Transfection Kit 200 Transfections
EpiFectagen® 上皮细胞转染试剂盒 Epithelial Cell Transfection Kit 100 Transfections
MesenFectagen® 间充质干细胞转染试剂盒 Mesenchymal Stem Cell Transfection Kit 250 Transfections
AstroFectagen® 星形胶质细胞转染试剂盒 Astrocyte Transfection Kit 250 Transfections
SMCFectagen® 平滑肌细胞转染试剂盒 Smooth Muscle Cell Transfection Kit 250 Transfections 【小鼠神经营养因子4(NT-4)ELISA试剂盒价格】
KeratoFectagen® 角质细胞转染试剂盒 Keratinocyte Transfection Kit 200 Transfections
EpiFectagen II® 上皮细胞转染试剂盒 Epithelial Cell Transfection Kit 250 Transfections 无货
IVTFA 体外管腔形成分析试剂盒 In Vitro Tube Fomation Assay (Angiogenesis Assay) 50 tests/96 well plate
Col I型胶原细胞粘附检测试剂盒 Collagen I Cell Adhesion Assay 48 tests/48 well plate
Fibro 纤维粘连细胞粘附检测试剂盒 Fibronectin Cell Adhesion Assay 48 tests/48 well plate
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