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本试剂盒仅供研究使用,齐一生物销售:021-6034 8496;181214 53965;173021 04490欢迎选购!
操作步骤
1.准备试剂,样品和标准品
2.加入准备好的样品和标准品,37℃反应30分钟
3.洗板5次,加入酶标试剂,37℃反应30分钟
4.洗板5次,加入显色液A、B,37℃显色15分钟
5.加入终止液
6.15分钟之内读OD值
7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。
【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】样本保存条件:
1.2-8℃冰箱最多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】样本收集
1.血清样本用干燥管(红色)或者促凝管(黄色)收集
2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
3.动物组织匀浆(细胞):
1g组织加入9ml PBS(浓度为0.1M,pH为7.4的)测分泌型指标
1g组织加入1ml组织裂解液加入8ml PBS 测细胞内
粪便样本:1g样本+9ml PBS(浓度为0.01M,pH为7.4的)
没有PBS的话也可以用一下生理盐水,最好还是用PBS
【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】样本离心转速及时间
1.试管采集的样本:3000转,离心五分钟取上清
2.离心管或者EP管采集的样本:5000--8000转3分钟取上清
3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】以上样本采集及处理方式供您参考,如有问题请来电,齐一生物销售:021-6034 8496;181214 53965;173021 04490
800341 Cosmid End-Sequencing, w/ prep CNY 【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】
800308 siRNA Hairpin Run w/ prep CNY
800121 Verification Sequencing, w/ prep "Double-stranded sequencing of a given sequence
QIAGEN® DNA template preparation
Design and synthesis of walking primers
Data assembly and sequence alignment
Accuracy >99.99%" CNY
132528 NeXtal Stock CAPSO untit. (50) 0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube) CNY
132510 NeXtal Stock ADA untit. (50) 0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube) CNY
764114 PAXgene Bone Marrow RNA Tubes (50) CNY
679913 ipsogen RT Kit (33) CNY
203643 HotStarTaq Plus Master Mix Kit (250) "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water
HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, and performance as HotStarTaq Plus DNA Polymerase NOW in a master mix format, which offers even faster results and added convenience." CNY
201225 Taq PCR Core Kit (1000) "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time." CNY
201203 Taq DNA Polymerase (250) "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time." CNY
180922 GeneRead Adapter L Set 1plex (12) CNY
180912 GeneRead Adapter I Set 1plex (12) CNY
159214 QIAcard FTA Indicator Four Spots (25) CNY
159201 QIAcard FTA One Spot (100) CNY
9235523 Needle cleaning assembly P/N 4567-01 CNY
9235300 Plunger tip, syringe 2,5ml CNY
9235117 Fuse F4A, v1.00 CNY 【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】
9022818 EZ1 Advanced XL Flip Cap Tube Rack CNY
9234889 Vacuum manifold, 96 seal CNY
9234862 System liquid container (2l Nalgene) CNY
9239555 PCBA, Laser Driver, Unlimited CNY
9238664 Hinge CNY
9238662 Lid with window CNY
9238659 Cooling compressor T2168GK, 60Hz CNY
9238645 Brake resistor 112 Ohm / 100 W CNY
9242061 Pipetting container, IQ/OQ, QS CNY
9241827 Pyro Q96 ID, Premium Agreement "Repair service delivery: onsite
Service response time (repair): 24 hours/1 business day
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) provision: not included
Transportation cost coverage: not applicable" CNY
9241166 Board, A/D, QX CNY
9240955 Sensor, z, handler, Qsym CNY
9240835 QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, and parts. With a Basic Agreement, there is a priority response time of 5 working days. CNY
9240766 32 OUT Module, D2-32TD1, AP96 CNY
6000-5018 MST STM Rack & Lid (Blue - 2 pos. cal.) CNY
MEF-mt 小鼠胚胎成纤维细胞--CF1小鼠C处理 Mouse Embryonic Fibroblasts from CF1 mouse 1 x 10^6 cells/vial
MEF-mt 小鼠胚胎成纤维细胞--CF1小鼠C处理 Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse 1 x 10^6 cells/vial
HDF-f-mt 人真皮成纤维细胞-胎儿C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial
MEF-cm 小鼠胚胎成纤维细胞培养基 Mouse Embryonic Fibroblasts-Conditioned Medium 100 ml
bFGF-std MEF-cm 碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基 bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium 100ml
ULBC plates 干细胞超低结合培养板 Ultra-Low Binding Culture Plate 6-well, 1 plate
ULBC plates 超低结合培养板(6孔) Ultra-Low Binding Culture Plate (6-well) "6-well, 4 plates
" 【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】
ULBC plate 超低结合培养板(24孔) Ultra-Low Binding Culture Plate (24-well) "24-well, 1 plate
"
ULBC plates 超低结合培养板(24孔) Ultra-Low Binding Culture Plate (24-well) "24-well, 4 plates
"
rhbFGF 重组人碱性成纤维细胞生长因子--碱性 Recombinant Human Fibroblast Growth Factor-basic 10µg
rhEGF 重组人表皮生长因子 "Recombinant Human Epidermal Growth Factor
" 100µg
rhBMP-2 重组人骨形成蛋白-2 "Recombinant Human Bone Morphogenetic Protein-2
" 2µg
rhBMP-4 重组人骨形成蛋白-4 "Recombinant Human Bone Morphogenetic Protein-4
" 2µg
rhBMP-7 重组人骨形成蛋白-7 "Recombinant Human Bone Morphogenetic Protein-7
" 2µg
rhNOGGIN 重组人NOGGIN蛋白 "Recombinant Human NOGGIN
" 5µg
HAMSC 人羊膜间充质基质细胞 Human Amniotic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
HCMSC 人绒毛膜间充质基质细胞 Human Chorionic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
HMSC-bm 人间充质干细胞--骨髓 Human Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial
HMSC-ad 人间充质干细胞--脂肪 Human Mesenchymal Stem Cells-adipose 5 x 10^5 cells/vial
HMSC-hp 人间充质干细胞--肝脏 Human Mesenchymal Stem Cells-hepatic 5 x 10^5 cells/vial
HUMSC 人脐带间充质干细胞 Human Umbilical Mesenchymal Stem Cells 5 x 10^5 cells/vial
HPMSC 人肺间充质干细胞 Human Pulmonary Mesenchymal Stem Cells 5 x 10^5 cells/vial
HVMSC 人脊椎间充质干细胞 Human Vertebral Mesenchymal Stem Cells 5 x 10^5 cells/vial 【小鼠超氧化物歧化酶(SOD)ELISA试剂盒价格】
MSCM 间充质干细胞培养基 Mesenchymal Stem Cell Medium 500 ml
MODM-500 间充质干细胞-成骨细胞分化培养基 Mesenchymal Stem Cell Oseogenic Differentiation Medium 500 ml
MODM-250 Mesenchymal Stem Cell Oseogenic Differentiation Medium 250 ml
MODM-b 间充质干细胞-成骨细胞分化培养基-基础 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal 500ml
MODM-b-prf 间充质干细胞-成骨细胞分化培养基-无酚红 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal-phenol red free 500ml
MADM-500 间充质干细胞-脂肪细胞分化培养基 Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml
MADM-250 Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml
小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格 QY-M30618 国产/进口原装 48T/96T 齐一生物科技(上海)有限公司销售:021-6034 8496;18121
小鼠甘油三酯(TG)ELISA试剂盒厂家 QY-M30617 国产/进口原装 48T/96T 齐一生物科技(上海)有限公司销售:021-6034 8496;181214
小鼠脂肪型脂肪酸结合蛋白4(FABP4)ELISA试剂盒价格
小鼠脂肪型脂肪酸结合蛋白4(FABP4)ELISA试剂盒价格 QY-M30616 国产/进口原装 48T/96T 齐一生物科技(上海)有限公司销售:021-6034 8496
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