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1.购买我公司任意ELISA试剂盒指标享受免费代测,并提供技术指导服务!
二、实验材料与试剂配制:
1. 仪器与材料:酶标仪(使用前预热30分钟),微量加液器、吸头、蒸馏水或去离子水,滤纸。
2. 缓冲液使用:加5ul 的缓冲液于50ul 的样品中,如果样品量不够或者不确定,缓冲液和样品的混合比例不要小于1:10即可混匀,静置1小时备用(如果标本是血清或者血浆,此步骤忽略)。
3. 洗液的配制:按1:100的比例配制洗液备用。
齐一生物销售:021-6034 8496;181214 53965;173021 04490
【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】三. 仔细阅读说明书
1.检查试剂盒标签上的有效期。如果超过有效期,请勿使用。
2. 按说明书确定所有试剂齐全(数量、体积)
3. 标本制备要规范,每份标本体积要按2-3个复孔以上的量制备(贮存),尽量分装做备份。
4.短期内无法实验者,注意低温保存
5.准备好所有实验额外所需物品,(比如移液器,试管,清洗器,酶标仪)
6. 按说明书将所用试剂平衡至室温,根据检测标本数量确定所需试剂的量
7.检查试剂盒内每种试剂的贮存条件,保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】
8.检查不稳定或者变质的试剂溶液(比如沉淀或变色)。有些试剂,比如标准品稀释液或者检测稀释液,可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前,必需充分混匀。而由于沉淀物的特性,在加入酶标板之前,必需不停搅拌。
9.保证充分的孵育时间和温度。
10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。
11.在混合或溶解蛋白溶液时,避免泡沫产生。
12.在实验开始之前,安排好实验流程。在实验开始之前,清洁工作台。
13.若有问题,应与我公司或代理商的技术支持联系
【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】四、客户须知
齐一生物销售:021-6034 8496;181214 53965;173021 04490
1.在收集标本前都必须有一个完整的计划,必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测,对收集后当天就进行检测的标本,及时储存在4℃备用,如有特殊原因需要周期收集标本,请造模取材后,将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差,蛋白极易降解,直接影响实验质量,所以避免反复冻融。部分标本也可加入适当防腐剂,激素类标本需添加抑肽酶。
2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书,具体操作注意事项请与我司技术人员沟通。
3.液体类标本:标本必须为液体,不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。
4.购买前请提前告诉我们:您样本的种属、样本数量、待测指标及其它特殊要求。
【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】五、代测数据详细内容
1、ELISA标准曲线;
2、实验具体详细步骤;
3、完整的实验报告(试剂耗材,实验方法,实验结果及结果说明);
4、实验进行阶段,我公司客服人员会及时向您汇报实验进程。
9239959 EZ1, Premium Agreement The Premium Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visits, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY 【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】
9020028 ZB sensor, 320mm, EZ1xl CNY
9020018 Upgrade kit, vacuum chamber, CAS-1820 CNY
9019987 PCB, small breakout, 8 core, Cxx CNY
9019927 Cover, tip ejector (UV), Cx8 CNY
9019926 PCB, Microboard, Cxx CNY
9019488 Filter, polycap incl. tube/coupling, CX1 CNY
9238856 #Basic Cover Agreement MDx, 2PM, 1yr The Basic Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes two annual preventive maintenance visits, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9238839 #Twister II, Basic Agreement, no PM The Basic Cover Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, and parts. With a Basic Cover Agreement, the response time is 5 working days. CNY
9241178 Fuse, 4A, 250VAC, QX CNY
9238830 BR 3000, Basic Agreement The Basic Cover Agreement 3000 provides full service coverage for the BioRobot 3000. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9238813 Rectangular carrier for 20 RIA-tubes CNY
9019471 Lid, Polycarb, C1x CNY
9237327 Upgrade, BR & 1 ext. device. w. sched. Upgrade BioRobot with one approved integration device using scheduling software CNY
9233197 Pump, vacuum, auto vac, BR8000 CNY
9237153 Syringe Pump CNY
9237111 Upgrade App., QIAamp 96 Virus On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Virus including kit CNY 【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】
9237104 Upgrade, BR plus 4 ext. devices Upgrade BioRobot with four approved integration devices CNY
9237085 LiquiChip, Installation The Installation, LiquiChip facilitates the quick implementation of the LiquiChip into the lab routine by minimizing the instrument start-up phase. It covers the hardware and software installation of the LiquiChip Advanced instrument and provides the user with an introductory training on operation and user maintenance. The installation includes labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY
9236420 Pins, rack alignment, set (5), TWI CNY
9236412 Fingers, landscape, kit, TWI CNY
9232975 Stirrer Adapter Board (Base Plate) PK80 CNY
9232952 YZ 96 Connector Board 3 (Arm), E10085 CNY
9236361 Alignment tube XYP CNY
9232938 Camera, buffer track, BR8000 CNY
9236347 250mA Fuse sensor distribution Board CNY
9236331 U-Block CNY
9236142 Disposal Chute, straight, BR Rapid Plate CNY
9230755 Rechen Stirrer Rose 80 CNY
9235701 Cable 5V, RCS1 CNY
9230433 Tubing Block CNY
9235298 Plunger tip, syringe 500µl CNY
└ 肾皮层上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 肾上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 输尿管上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 肾实质细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 肾系膜细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 膀胱上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 膀胱平滑肌细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 肾动脉内皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 肾动脉平滑肌细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 肾成纤维细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 膀胱成纤维细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 尿道上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 胎盘羊膜细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 胎盘滋养层细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 胎盘绒毛膜细胞总DNA 1μg/1μl(10μg/20ug/50ug) 【小鼠肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒厂家】
└ 子宫平滑肌细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 子宫成纤维细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 卵巢上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 卵巢成纤维细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 卵巢微血管内皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 子宫内膜上皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 淋巴内皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 淋巴成纤维细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 淋巴血管内皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 脐带单核细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 外周血白细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 颅盖造骨细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 滑膜细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 骨骼肌细胞总DNA 1μg/1μl(10μg/20ug/50ug)
└ 真皮微血管内皮细胞总DNA 1μg/1μl(10μg/20ug/50ug)
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