小鼠甲基化酶(Methylase)ELISA试剂盒价格

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【小鼠甲基化酶(Methylase)ELISA试剂盒价格】样本保存条件：

1.2-8℃冰箱最多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【小鼠甲基化酶(Methylase)ELISA试剂盒价格】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，最好还是用PBS

【小鼠甲基化酶(Methylase)ELISA试剂盒价格】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【小鼠甲基化酶(Methylase)ELISA试剂盒价格】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

330612    RT² SYBR Green Fluor FAST Mastermix (12)           CNY                            【小鼠甲基化酶(Methylase)ELISA试剂盒价格】

330521    RT² SYBR Green ROX qPCR Mastermix (8)              CNY                           

59665     EpiTect Control DNA, unmethylated (100)          CNY                           

9010046  Modul, Incubator, 6 MTP, P3xxx         CNY                           

9010024  Incubator/stacker 4/4 plates Plato 1/3          CNY                           

206544    Type-it HRM PCR Kit (400)         CNY                           

206243    Type-it Microsatellite PCR Kit (200)     "The Type-it Microsatellite PCR Kit is developed for fast ａnd reliable genotypic analysis of humans, animals, plants, ａnd bacteria using

microsatellites, STR, or VNTR markers without the need for lengthy optimization procedures

Features

 Reliable microsatellite analysis by multiplex PCR

 Microsatellite assay development without optimization

 Successful ａnd specific coamplification of all fragments

 Optimized protocols for any detection platform"     CNY                           

9012737  Syringe pump outlet tubing (4.7m)              CNY                           

9012720  Set of Pipet Tubing Plato 3001             CNY                           

9012717  Troughs, 50ml (100 troughs + lids)             CNY                           

133237    NeXtalStock Sodium citrate pH 5.4 (200)           CNY                           

133236    NeXtalStock Sodium citrate pH 5.2 (200)           CNY                           

9002193  QIAcube (230V), PrioPLUS, no PM     "QIAcube (230V) Instrument

Installation of QIAcube  instrument & Introductory training

3 years coverage on parts, labor ａnd travel

On site service, 48 hr response

No Preventive Maintenance

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service"      CNY                           

9002033  Rotor-Gene Q MDx 5plex HRM System             CNY                           

59205     EpiTect Whole Bisulfitome Kit (100)            CNY                           

59124     EpiTect Plus DNA Bisulfite Kit (48)             CNY                           

929551    QX DNA Size Marker FX174/HaeIII (50 µl)        CNY                            【小鼠甲基化酶(Methylase)ELISA试剂盒价格】

929528    QX Alignment Marker 50 bp/3 kb (1.5 ml)          CNY                           

972807    PyroMark Gold Q96 Reagents (50 x 96)      For performing pyrosequncing reaction on the Q96 MD     CNY                           

972442    PyroMark Q96 SNP HFE (96)      For detection of variants in HFE gene   CNY                           

133221    NeXtalStock Sodium ac. H2O pH 5.6 (200)        CNY                           

133220    NeXtalStock Sodium ac. H2O pH 5.0(200)         CNY                           

301704    HiPerFect Transfection Reagent (0,5 ml)            CNY                           

301427    Effectene Transfection Reagent (4x1ml)      4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates CNY                           

51326     QIAamp DNA Mini QIAcube Kit (240)        CNY                           

9012672  BGR Peripumpe PP-4 Frontbl.gerade (7135)              CNY                           

9002011  Rotor-Gene Q MDx 2Plex HRM no PC        CNY                           

292063    mericon Sheep Kit (24)         CNY                           

9001912  PyroMark Q24 MDx, PrioPLUS    "PyroMark Q24 MDx Instrument

Installation of PyroMark Q24  & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"       CNY                           

9001910  QIAgility HEPA/UV (w/o PC) PrioPLUS      "QIAgility HEPA/UV (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"   CNY                           

133116    NeXtalStock Sodium thiocyanate (200)        CNY                           

967142    RNeasy 96 BioRobot 9604 Kit (12)       For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd Buffers     CNY                           

QT-00204       乳酸片球菌JCM8791=DSM20284=NCIMB12174     冻干粉                               

QT-00205       桃色欧文氏菌NBRC102418 冻干粉                               

QT-00206       白色噬琼胶菌NBRC102603 冻干粉                               

QT-00207       浮游球衣菌NBRC13543      冻干粉                               

QT-00208       丁香假单胞菌NBRC14078   冻干粉                               

QT-00209       睾丸酮丛毛单胞菌NBRC14951   冻干粉                               

QT-00210       耐放射异常球菌NBRC15346      冻干粉                               

QT-00211       烟酸芽孢杆菌NBRC15566   冻干粉                               

QT-00212       新鞘氨醇单胞菌NBRC16725      冻干粉                               

QT-00213       假交替单胞菌NCCB100134 冻干粉                               

QT-00214       腐败希瓦氏菌NCIMB12202 冻干粉                                【小鼠甲基化酶(Methylase)ELISA试剂盒价格】

QT-00215       食油假单胞菌NCIMB6576  冻干粉                               

QT-00216       普通变形杆菌NICPBP49027       冻干粉                               

QT-00217       金龟子绿僵菌小孢变种NRRL13969   冻干粉                               

QT-00218       大孢绿僵菌=金龟子绿僵菌大孢变种NRRL13970    冻干粉                               

QT-00219       黄绿绿僵菌NRRL13971      冻干粉                               

QT-00220       黄绿绿僵菌NRRL20422      冻干粉                               

QT-00221       抗生链霉菌NRRL3238 冻干粉                               

QT-00222       抗生链霉菌NRRL8044 冻干粉                               

QT-00223       绿脓假单胞菌(绿脓杆菌)CMCC10104       冻干粉                               

QT-00224       金黄色葡萄球菌CMCC26003     冻干粉                               

QT-00225       枯草芽孢杆菌CMCC63501  冻干粉                               

QT-00226       大肠埃希氏菌(大肠杆菌)CMCC44102       冻干粉                               

QT-00227       乙型副伤寒沙门氏菌CMCC50094     冻干粉                               

QT-00228       短小芽孢杆菌CMCC63202  冻干粉                               

QT-00229       藤黄微球菌CMCC28001     冻干粉                               

QT-00230       费氏志贺氏菌CMCC51574  冻干粉                                【小鼠甲基化酶(Methylase)ELISA试剂盒价格】

QT-00231       阴沟肠杆菌CMCC43501     冻干粉                               

QT-00235       产气肠杆菌CMCC45103     冻干粉                               

QT-00236       甲型副伤寒沙门氏菌CMCC50093     冻干粉