小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家

ELISA 检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。齐一生物销售：021－6034 8496；181214 53965；173021 04490

注意事项：

◆   每个标本量收集体积＝  100ul × 检测种类，如要做复孔，标本量收集体积＝  100ul × 检测种类×2 。

◆   对于作某一个具体指标来说，最好先用一系列稀释度作一批标本测定，得出对实验有意义的一个稀释度（即测定剂量反应曲线），然后用一个最适稀释度测定即可。

◆   收集标本前必须清楚要检测的成份是否足够稳定。对收集后当天进行检测的标本，储存在4℃备用，如有特殊原因需要周期收集标本，将标本及时分装后放在-20℃ 或-70℃ 条件下保存。避免反复冻融。标本 2 -8 ℃ 可保存48 小时.-20℃ 可保存1个月。 -70 度可保存6个月。部分激素类标本需添加抑肽酶。

◆   血清标本采集时，应注意避免溶血，红细胞溶解时会释放出具有过氧化物酶活性的物质，以 HRP为标记的 ELISA 测定中，溶血标本可能会增加非特异性显色。

【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】◆   为了保证尿液检测结果的准确性，必须正确收集尿液标本和保存。盛尿容器要清洁干燥。最好使用一次性的容器（如塑料尿杯），避免因用药并清洗不干净而造成的污染，影响检测结果。尿液标本必须新鲜，留取后，应及时检测或保存，以免细菌繁殖。因在室温中久置后（尤其是夏季）尿中磷酸盐等可析出结晶而干扰检测。

◆    标本宜在新鲜时检测。如有细菌污染，菌体中可能含有内源性 HRP，也会产生假阳性反应。保存过久可发生聚合在 ELISA中可使本底加深。

◆   冻结标本融解后，蛋白质局部浓缩，分布不均，应充分混匀宜轻缓，避免气泡，可上下颠倒混和，不要在混匀器上强烈振荡。

◆   混浊或有沉淀的标本应先离心或过滤，澄清后再检测。

◆   反复冻融会使蛋白效价降低，所以待测标本如需保存作多次检测，宜少量分装冰存。也可加入适当防腐剂。

◆   如果您在实验中遇到任何特殊问题，敬请咨询 标本类型

ELISA常见的标本一般包括：

◆   液体类标本：包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。

◆   培养细胞

◆   组织标本

这些标本收集的时间、方法和保存都有一定的要求。

【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】标本的保存

一般说来，在 5 天内测定的标本可放置于 4℃，超过一周测定的需低温冻存。

对收集后当天进行检测的标本，储存在  4 ℃ 备用，如有特殊原因需要周期收集标本， 将标本及时分装后放在  -20 ℃ 或  -70 ℃ 条件下保存。避免反复冻融。 标本  2 -8 ℃ 可保存  48 小时，-20 ℃ 可保存  1 个月。-70 度可保存  6 个月。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。标本的处理 可用作 ELISA 测定的标本十分广泛，体液（如血清）、分泌物（唾液）和排泄物（如尿液）、细胞培养上清、细胞、组织等均可作标本以测定其中某种成份。有些标本可直接进行测定，有些则需经预处理。

◆ 血清：  

室温血液自然凝固  10-20 分钟后，离心  20 分钟左右（  2000-3000 转  / 分）。收集上清。如有沉淀形成，应再次离心。  

◆ 血浆：  

应根据试剂盒的要求选择  EDTA 、柠檬酸钠或肝素作为抗凝剂，加入  10 ％（  v/v ）抗凝剂 ( 0.1M 柠檬酸钠或  1% heparin 或  2.0%EDTA.Na2) 混合  10-20 分钟后，离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清如有沉淀形 成，应再次离心。  

【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】◆ 尿液、胸腹水、脑脊液：  

用无菌管收集。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清。如有沉淀形成，应再次离心。  

◆ 细胞培养上清：  

检测分泌性的成份时，用无菌管收集。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清。检测细胞内的 成份时，用  PBS （  PH7.2-7.4 ）稀释细胞悬液，细胞浓度达到  100 万 /ml 左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心  20 分钟左右（  2000-3000 转 / 分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。  

◆ 细胞：

检测细胞的成份时，对于贴壁细胞，去除培养液，用PBS、生理盐水或无血清培养液洗一遍。加入适量裂解液。用枪吹打数下，使裂解液和细胞充分接触。通常裂解液接触细胞10 秒后，细胞就会被裂解。对于悬浮细胞，离心收集细胞，用PBS、生理盐水或无血清培养液洗一遍.加入适量裂解液, 用枪吹打把细胞吹散。用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多，必需分装然后再裂解。充分裂解后，10000-14000g离心3-5 分钟，取上

清，即可进行后续操作。

【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】◆ 组织标本：  

切割标本后，称取重量。加入一定量的  PBS或组织蛋白萃取试剂，缓冲液中可加入蛋白酶抑制剂。用手工或匀浆器将标本匀浆充分。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清置于  -20 度或  - 70 度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。齐一生物销售：021－6034 8496；181214 53965；173021 04490  

293023    mericon Corn (24)         CNY                            【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】

292055    mericon Goat Kit (96)           CNY                           

929521    QX Alignment Marker 15 bp/1 kb (1.5 ml)          CNY                           

9012598  Disposable Filter-Tips, 1100µl (960)     Conducting disposable filter-tips    CNY                           

206152    QIAGEN Multiplex PCR Plus Kit (100)        CNY                           

291115    mericon RR Soy Kit (96)              CNY                           

291065    mericon GMO Screen bar (96)            CNY                           

9001933  DML 3000 120-240V Q        CNY                           

9001915  PyroMark Q96 ID, Priority    PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

133120    NeXtalStock Tricine (200)           CNY                           

133180    NeXtalStock Citric acid pH 3.0 (200)          CNY                           

9018503  Manual air supply, service tool, QX             CNY                           

51161      QIAamp 96 DNA Blood Kit (4)     For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                           

133106    NeXtalStock Sodium ac. H2O pH 4.6 (200)        CNY                           

9015519  Spring, for door            CNY                           

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority     QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001875  EZ1 Advanced XL, Priority    EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                            【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】

959236    MagAttract RNA Tissue Mini M48 Kit (192)        CNY                           

956336    MagAttract RNA Univ. Tissue M48 Kit(192)        CNY                           

9018457  Cartridge, alignment tool, QX              CNY                           

9015467  Motor, P         CNY                           

9015451  Modules, DISU, BR8000 v2.1              CNY                           

9012427  Thermostate system, complete, 120V, BR30        CNY                           

9018483  Notebook, QX              CNY                           

51297     FlexiGene DNA AGF3000 Kit (640)      "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"   CNY                           

51185      QIAamp DNA Blood Midi Kit (100)      For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) CNY                           

38075     AIL Cartridge (5ml)              CNY                           

9015471  Sensor, photo, PM-U24         CNY                           

956034    EZ1 RNA Universal Tissue Kit (48)      Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.      CNY                           

204772    Rotor-Gene Multiplex PCR Kit (80)      The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.  CNY                           

9018435  Tubing, external N2 source, 10ft, QX          CNY                           

9018417  Cover, front, blue window, QX            CNY                           

QY-10622       Anti-Caspase-6 （NT）半胱胺酸蛋白酶蛋白-6抗体(N端)                                        【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】

QY-10632       Anti-Caspase-8 (pro Mch5)半胱氨酸蛋白酶8抗体                                     

QY-10642       Anti-Caspase-9白介素1-β转化酶样凋亡蛋白酶6抗体                                    

QY-10652       Anti-Caspase-9白介素1-β转化酶样凋亡蛋白酶6抗体                                    

QY-10662       Anti-β-catenin/β-catsβ-连环蛋白抗体                                      

QY-10672       Anti-NAIF1 protein（ CB12-327）核凋亡诱导因子蛋白1                                         

QY-10682       Anti-CBFA1/RUNX2 (Runt-related Transcription Factor 2)成骨特异性转录因子抗体                                       

QY-10692       Anti-CCK8(Cholecystokinin-8)胆囊收缩素抗体/缩胆囊素抗体(八肽)                                        

QY-10702       Anti-CCP (cyclic citrullinated peptide)兔抗瓜氨酸环肽抗体                                        

QY-10712       Anti-CCR-1(CC chemokine receptor 1)CC趋化因子受体1抗体                                        

QY-10722       Anti-CCR-2/5(CC chemokine receptor 2/5)CC趋化因子受体2/5抗体                                       

QY-10732       anti-CCR5/RANTES（regulated on activation normal T cell expressed ａnd secreted）CC趋化因子受体5/依赖激活T细胞分泌调节蛋白                                         

QY-10742       Anti-CCR-3(CC chemokine receptor 3)CC趋化因子受体3抗体                                        

QY-10752       Anti-CCR-4(CC chemokine receptor 4)CC趋化因子受体4抗体                                        

QY-10762       Anti-CCR-7(CC chemokine receptor 7) CC趋化因子受体7抗体                                       

QY-10772       Anti-CD10（Mouse Anti-Human CD10 Monoclonal Antibody）鼠抗人CD10单克隆抗体                                        

QY-10782       Anti-CD10/CALLA (Neutral Endopeptidase)CD10抗体                                         【小鼠前心钠肽(Pro-ANP)ELISA试剂盒厂家】

QY-10792       Anti-CD105/Endoglin/TGF-β receptorCD105/转化生长因子β受体抗体                                        

QY-10802       Anti-CD117/c-kit/SCFR干细胞生长因子受体/细胞表面分化抗原抗体                                     

QY-10812       Anti-CD133 antigen造血干细胞抗原CD133抗体                                       

QY-10822       anti-Syndecan-1/CD138多配体聚糖抗体                                      

QY-10832       Anti-CD14(cluster of differentiation antigen 14)CD14抗体                                   

QY-10842       anti-CD28CD28抗体                                       

QY-10852       Anti-CD144/VECD （Vascularendothelial cell cadherin/VE-cadherin）上皮型钙粘附分子/血管内皮钙粘蛋白抗体                                     

QY-10862       Anti-CD147/EMMPRIN(Extracellular matrix metalloproteinase inducer)单核-巨噬细胞基质金属蛋白酶抗体                                         

QY-10872       anti-CD166/ALCAM(activated leukocyte celladhesion molecule)活化白细胞粘附分子抗体                                       

QY-10882       anti-ED-1单核/巨噬细胞表面特异性标志抗原抗体                                    

QY-10892       Anti-CD167a/DDR1CD167a抗体                                   

QY-10902       Anti-CD169/Siglec-1/sialoadhesin（Sn）(Sialic acid-binding Ig-like lectin 1)CD169抗体                                   

QY-10912       Anti-CD17CD17抗体