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【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】酶法检测试验所需自备物品:
1. 酶标仪(450nm 波长滤光片)
2. 高精度移液器,EP 管及一次性吸头
3. 37℃恒温箱,双蒸水或去离子水
4. 吸水纸
试剂盒安全性提示:
1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体,请尽快用水冲洗。
2.实验中不要吃喝、抽烟或使用化妆品。
3.不要用嘴吸取试剂盒里的任何成份。
【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】操作注意事项:
1.试剂盒应按标签说明书储存,使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃,不可保存。
2.实验中不用的板条应立即放回包装袋中,密封保存,以免变质。
3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。
4.使用一次性的吸头以免交叉污染,吸取终止液和底物A、B液时,避免使用带金属部分的加样器。
5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6.洗涤酶标板时应充分拍干,不要将吸水纸直接放入酶标反应孔中吸水。
7.底物A应挥发,避免长时间打开盖子。底物B对光敏感,避免长时间暴露于光下。避免用手接触,有毒。实验完成后应立即读取OD值。
8.加入试剂的顺序应一致,以保证所有反应板孔温育的时间一样。
9.按照说明书中标明的时间、加液的量及顺序进行温育操作。
【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】齐一生物客户寄标本时需注明以下情况:
1、标本编号;2、所测项目;3、是否做复孔;3、联系方式;4、实验后标本是否寄回。
客户必知:
客户应对所提供的材料及信息负责,如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。
样本保存条件:
1.2-8℃冰箱最多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
样本收集
1.血清样本用干燥管(红色)或者促凝管(黄色)收集
2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
3.动物组织匀浆(细胞):
1g组织加入9ml PBS(浓度为0.1M,pH为7.4的)测分泌型指标
1g组织加入1ml组织裂解液加入8ml PBS 测细胞内
粪便样本:1g样本+9ml PBS(浓度为0.01M,pH为7.4的)
没有PBS的话也可以用一下生理盐水,最好还是用PBS
样本离心转速及时间
1.试管采集的样本:3000转,离心五分钟取上清
2.离心管或者EP管采集的样本:5000--8000转3分钟取上清
3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】齐一生物供应,以上资料供参考如有疑问需要详细资料请联系我们客服人员,齐一生物销售:021-6034 8496;181214 53965;173021 04490
9241815 Pyro Q96 ID, PM Agreement CNY 【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】
9241172 anti-rotation tool CNY
9240960 Cable, cLLD, pipetting system, Qsym CNY
9240949 Cable, y, portal, Qsym CNY
9240678 Bimba Rotary Actuator, AP CNY
9240777 CE Update, AP196/98 CNY
9241773 RGQ, Installation The Installation and Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware and trains the user on the operation of the Rotorgene software. After the training the user will be able to develop application programs, conduct a basic troubleshooting, and understand the application output format.All travel and labor costs and well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements. CNY
9241763 QIAgility, Full Agreement "Repair service delivery: onsite
Service response time (repair): 48 hours/2 business days
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for repair parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) or PC repair provision: not included
Transportation cost coverage: not applicable" CNY
9241372 Reflector, UV lamp, EZ1a CNY
9243545 QIAsymphony SP, PM Service "Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9243045 Bracket, rear, transparent panel, Qsym CNY
9242980 QIArent&go - Rent out fee QS RGQ System CNY
9242764 QS RGQ, Premium Agreement "Repair service delivery QIAsymphony SP/AS: onsite
Service response time (repair) QIAsymphony: 24 hours/1 business day
Repair service delivery RGQ: send to Regional Repair Center
Service response time (repair) RGQ: 7-10 days turnaround time
Periodic inspection/maintenance: yes, 1/agreement year
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)
Transportation cost coverage: yes, full coverage
QIAsymphony Response Time starts when customer inquiry is received by local tech service ends when a service specialist is onsite.
- Inquiry Channel: Local Tech-Service Hotline, Mo.- Fr. 08:00-17:00 Uhr
- Call Cut-off Time: 12:00 am; on business days
- Customer Availability: maximum 2 hours phone trouble shooting to identify error and spare parts
- QIAsense: Systems need to be connected to QIAsense for faster download of log files
- Software: QIAsymphony need to be updated with the latest software version
- Remote Area: 24 Hr onsite response only available for geographical areas within field service travel capability and pre-12:00 delivery capability of spare parts" CNY
9242750 Cooling adapter, PCR adapter, AS, Qsym CNY
9242709 Validation Support, 2d, EZ1 The QIAGEN EZ1 Guided Validation Support service includes all travel and labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the EZ1 system and the DNA Investigator silica chemistry utilized in the process, review the Handbook and User Manual, and practical hands-on operation. The Guided Validation service includes experimental design consultation, internal validation documentation support, and QIAGEN sponsored data templates. The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process. Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design and are not included in the cost of validation support. CNY
9242699 QIAxcel, Full Agreement "Repair service delivery send to Regional Repair Center
Service response time (repair) 7-10 days turnaround time
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery:
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: yes, included (provision time 2-3 days)
Transportation cost coverage: yes, full coverage" CNY
9242508 QIAsymphony SPAS, Premium Agreement "Repair service delivery: onsite
Service response time (repair): 24 hours/1 business day
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: not applicable
- QIAsense: Systems should to be connected to QIAsense for faster download of log files" CNY 【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】
5000-1316 Rotary Shaker - Knob Locking CNY
9241365 Grid, UV lamp, EZ1a CNY
9240761 CPU, AP96 CNY
9240757 Downtube, 500 ml Bottle, AP96/196 CNY
9242768 Belt, x-drive, portal, SP, Qsym CNY
9242715 Validation Support, 5d, EZ1 QThe QIAGEN EZ1 Accelerated Validation Support service includes all travel and labor charges for a site visit up to 5 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the EZ1 system and the DNA Investigator silica chemistry utilized in the process, review the Handbook and User Manual, as well as hands-on user support and EZ1 unit operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, and QIAGEN sponsored data templates. The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR and CE setup, normalization, etc. to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation and report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design and are not included in the cost of validation support. Attendees will receive a certificate of participation in the hands-on exercises. CNY
5000-1314 Rotary Shaker - Bar Roller (Pair) CNY
5000-1305 Switch Power, SKR1 CNY
5000-1281 DML 2000 - Power Cord 220V B Type (DE) CNY
5025-1015 Expand 4 Pipettor Stand CNY
6000-3118 Plate Washer I - Wash Cap Assembly CNY
6000-1203 96 Well Plate CNY
5000-1418 Control Digene PCB, VTX2 CNY
5140-1220 hc2 GC-ID DNA Test CNY
5050-1076 V.1 HYBRID CAPTURE SYSTEM CNY
QY-x1296 人组织细胞淋巴瘤细胞U937
QY-x1297 人急性T淋巴母细胞白血病细胞MOLT-4
QY-x1298 人T细胞淋巴瘤Hat-78
QY-x1299 人T细胞淋巴瘤Hurt-78
QY-x1300 人T淋巴细胞系H9
QY-x1301 人T淋巴细胞白血病,CD8+Molt-4
QY-x1302 人B淋巴母细胞HMy2.CIR
QY-x1303 人急性淋巴细胞白血病T淋巴细胞CCRF-CEM
QY-x1304 总T淋巴细胞OKT3
QY-x1305 人大动脉平滑肌细胞HASMC
QY-x1306 人脉络丛上皮细胞HCPEpiC 【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】
QY-x1307 人血管内皮细胞VE
QY-x1308 人血管内皮细胞EC-304
QY-x1309 人脐静脉血管内皮细胞HUVEC
QY-x1310 人脐静脉血管内皮细胞HUVEC(原代)
QY-x1311 人脐静脉内皮细胞HUVE-12/CRL2480
QY-x1312 人脐静脉内皮细胞ECV-304
QY-x1313 人脐静脉内皮 (具有T24细胞特性)ECV-304
QY-x1314 人肠系膜下腔动脉血管内皮细胞Ealy926
QY-x1315 人脑胶质细胞株(正常)HEB
QY-x1316 人脑瘤细胞SF126
QY-x1317 人脑瘤细胞SF763
QY-x1318 人脑瘤细胞SF767
QY-x1319 人脑瘤细胞SF17
QY-x1320 人脑胶质母细胞瘤细胞TG-905
QY-x1321 人胶质瘤细胞TJ905
QY-x1322 人神经母细胞瘤细胞IMR-32 【大鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒厂家】
QY-x1323 人神经胶质瘤细胞U251
QY-x1324 神经胶质瘤细胞 CRT
QY-x1325 人胶质母细胞瘤细胞A172
QY-x1326 神经胶质瘤细胞 SHG-44
rm� 0 saI�oG-US>9017686 AUTOPURE Waste Container CNY
9017660 Sample Carrier, plate, Qsym CNY
9020255 USB-A-B port, Qsym CNY
9020238 Slideout electrical tray (110 V), C1x CNY
9020230 Slideout electrical tray, SMPS, 12Q CNY
9020208 PCB, lrge breakout, non LLS, CC2 CNY
9020196 Clamp, worktable (marked "I"), CX1 CNY
9020185 Gasket, centrifuge lock, QIAC CNY
9020097 Handwheel, TLyser 2 CNY
9237166 Serial Cable, 5ft CNY
9233016 Datakabel Scanner extern PK80 CNY
9237114 Upgrade App., Tube-Ring Setup CNY
9237113 Protocol Dev./Opt., 1-day extension During the scheduled week of the visit, additional days may be purchased for further assistance with your instrumentation system. CNY
9237088 Installation, BR RP plus BR TWI Installation and training of BioRobot Rapid Plate with Twister 1 Stand-Alone. CNY
9237087 Installation, BR RP Installation and training of Rapid Plate Stand Alone CNY 【大鼠肺表面活性物质相关蛋白B(SP-B)现货】
9236417 Software, Twister I, TWI CNY
9236264 Elution Microtubes Adaptor, inject mold CNY
9236165 O-ring, lubrication kit, RapidPlate CNY
9235931 Splashing guard, BR8 RD CNY
9230822 Rack for reagents,Trough (for 6 troughs) CNY
9235707 Washer Cable, RCS1 CNY
9235695 Ground Wire, RCS1 CNY
9235332 Z-Rack Packing Cover CNY
9235118 Fuse F1A, v1.00 CNY
9235114 Fuse FST4A, v1.00 CNY
9230054 Clamp, Tubing, Z-bar CNY
9234861 Lid coupling piece for system liquid CNY
9239551 Cbl Assy, XY, LED Driver CNY
9238663 Hook, motorized lid lock CNY
9238642 Capacitor board CNY
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QY-x1236 前列腺癌细胞LncaP
QY-x1237 非雄激素依赖型前列腺癌Tsu-Prl
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QY-x1243 白介素-2转染耐VP16绒癌细胞JEG-3/VP16-IL-2
QY-x1244 TNFa转染耐VP16绒癌细胞JEG-3-VP16-TNFa
QY-x1245 人前列腺癌细胞ALVA
QY-x1246 人前列腺癌细胞gpc-1
QY-x1247 人前列腺癌细胞9L-B
QY-x1248 人前列腺癌细胞9L-E
QY-x1249 人前列腺癌细胞JCA-1
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QY-x1252 人前列腺上皮细胞RWPE2
QY-x1253 人男性正常龟头细胞系HS68
QY-x1254 小儿包皮细胞HFF
QY-x1255 人正常前列腺上皮细胞RWPE-1
QY-x1256 人慢性髓原白血病细胞K562
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QY-x1260 人红白细胞白血病细胞ErythroleukemiaHEL
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