小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家

齐一生物试剂盒组分经过重重优化，每个组分的稳定性都完美的经过高温破坏测验，最大程度的避免了长途运输和保存过程中抗原抗体的失活.齐一生物试剂盒凭借其完美的标曲.严格的质控.稳定高、易保存，操作简便，已广泛应用在全国免疫学检验研究所的各领域中.深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】齐一生物试剂盒凭借其完美的标曲、严格的质控、较高的稳定性，在世界范围内的科研试剂领域中脱颖而出深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

操作步骤
实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。
1.         加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。
为保证实验结果有效性，每次实验请使用新的标准品溶液。
2.         弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。
3.         温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，350μl/每孔，甩干。
4.         每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。
5.         温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔梯度不明显，即可终止）。
7.         依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。
8.         用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。
【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】注：
1. 用户在初次使用试剂盒时，应将各种试剂管离心数分钟，以便试剂集中到管底。
2. 每次实验留一孔作为空白调零孔，该孔不加任何试剂，只是最后加底物溶液及2N H2SO4。测量时先用此孔调OD值至零。
3. 为防止样品蒸发，试验时将反应板放于铺有湿布的密闭盒内，酶标板加上盖或覆膜。
4. 未使用完的酶标板或者试剂，请于2-8℃保存。标准品、生物素标记抗体工作液、辣根过氧化物酶标记亲和素工作液请依据所需的量配置使用。请勿重复使用已稀释过的标准品、生物素标记抗体工作液或、辣根过氧化物酶标记亲和素工作液。
5. 建议检测样品时均设双孔测定，以保证检测结果的准确性。
以上资料仅供您参考，如有问题欢迎来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

9239566 Packaging, LX100 Optics Platform        CNY             【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】

9239558 Spring, XYP Torsion Door (Set of 5)     CNY            

9019925 PCB, Mainboard (Microbrd not incl.), Cxx        CNY            

9238867 Premium Cover Agreement MDx, 2PM, 1yr   The Premium Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY            

9241213 Full Agreement Depot Repair, PtWash, 1yr    "The Full Agreement Depot Repair, PtWash provides full service coverage for the plate washer used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."   CNY            

9241199 QIAxcel, Full Agreement, no PM  The Full Depot Repair Agreement, QIAxcel covers all shipping, labor ａnd repair part costs related to an instrument repair for 1 year. Service turn around time is 7-10 working days.   CNY            

9238821 Rect.  carrier for 2 Falcon tubes 50 ml     CNY            

9019469 COMB CASTING 0.2MM X 52 WELLS       CNY            

9243649 Acceler. Valid Support, Other Quant. Sys    The QIAGEN Accelerated Validation Support Other Quantification System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)  CNY            

9233373 Spring, gas-driven, cover, BR8000       CNY            

9233339 Valve, throttle     CNY            

9237335 BR MDx, Full Agreement  The Full Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).   CNY            

9237148 XYP Fan     CNY            

9237136 #Twister II, Full Agreement, no PM  The Full Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY            

9237128 Upgrade App., RNeasy 96, BR8000     CNY            

9237119 Upgrade App., MagAttract Miniprep       CNY            

9237105 Protocol Dev./Opt., on-site (1-day) The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 8 hours by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed. CNY             【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】

9236419 Buzzer, TWI     CNY            

9236411 Fingers, deepwell, kit, TWI     CNY            

9232967 Board, X-main adapter, BR8000 v1.00     CNY            

9232951 Bar code reader adapter Board, E10084       CNY            

9236360 Syringe Cylinder with Seal  "syringe cylinder ａnd seal for Luminex. We apparently do not sell the seal separate from the cylinder. These items are replaced at annual PM＇s, but customer＇s may want to replace them more often ａnd would be responsible for paying for them on their own then.

Source: Glen"   CNY            

9232920 Card, x-main, PCON, v1.04       CNY            

9232914 PCB, RS232, X-main      CNY            

9236349 375mA Fuse sensor distribution Board        CNY            

9236332 Compressor      CNY            

9236322 Air Solenoid        CNY            

9236162 O-Ring, replacement kit, RapidPlate     CNY            

9236152 Locator Plate, Tips, BR Rapid Plate     CNY            

9236147 Disposal Chute, side, BR Rapid Plate        CNY            

9230778 Spritzschutz Waschstation Plato 7       CNY            

9230776 Scannerdeckel Rose 80       CNY            

QY-00470    Insulin Receptor-α（ISR-α）胰岛素受体-α（抗原）       0.5mg/6501mg/1100             

QY-02600    Insulin Receptor-α（ISR-α）胰岛素受体-α（抗原）       0.5mg/6501mg/1100             

QY-02900    Insulin Receptor-β（ISR-β）胰岛素受体-β（抗原）       0.5mg/6501mg/1100             

QY-01720    IRS-1胰岛素受体底物-1（抗原）        0.5mg/6501mg/1100             

QY-01730    IRS-2 胰岛素受体底物-2（抗原）       0.5mg/6501mg/1100              【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】

QY-01860    IRS-3胰岛素受体底物-3（抗原）        0.5mg/6501mg/1100             

QY-01870    IRS-4胰岛素受体底物-4（抗原）        0.5mg/6501mg/1100             

QY-02420    IRS P53 (Insulin receptor suRCtrate P53)(多肽）      0.5mg/6001mg/1000             

QY-03420    ITG-β3(Integrin β chain,β3 precursor)整合素-β3（抗原）       0.5mg/6501mg/1100             

QY-01080    Leptin瘦素（多肽抗原）       0.5mg/6501mg/1100             

QY-01090    Leptin receptor（long）瘦素受体（长）（抗原）         0.5mg/6501mg/1100             

QY-00220    MAPK-1/2丝裂原活化蛋白激酶（抗原）       0.5mg/6501mg/1100             

QY-02230    MAPKK2丝裂原活化蛋白激酶激酶         0.5mg/6501mg/1100             

QY-01010    M2-PK （ pyruvate Kinase M2）丙酮酸激酶-M2（抗原）       0.5mg/6501mg/1100             

QY-01020    M2-PK （ pyruvate Kinase M2 ） 丙酮酸激酶-M2（抗原）         0.5mg/6501mg/1100             

QY-00510    Melan-A/MART-1黑色素-A（抗原）       0.5mg/6501mg/1100             

QY-08040    ARIP2a（Activin receptor 2A）激活素受体2A/激活素受体相互作用蛋白2a抗原       0.5mg/6501mg/1100             

QY-08570    β-arrestin 1/2(beta-arrestin 1/2)β-抑制蛋白1/2抗原         0.5mg/650             

QY-08010    ARIP2B（Activin receptor 2B）激活素受体2B/激活素受体相互作用蛋白2b抗体       0.5mg/6501mg/1100             

QY-00270    MTR-1A (Melatonin receptor-1A)褪黑素受体（抗原）         0.5mg/6501mg/1100             

QY-03340    MTLC (c-Myc target from laryngeal cancer cells)又称：MYC致癌基因（抗原）         0.5mg/6501mg/1100              【小鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒厂家】

QY-02240    N-coR1 （Nuclear receptor co-repressor 1） 核受体辅助抑制因子（抗原）        0.5mg/6501mg/1100             

QY-00060    Nestin巢蛋白（神经上皮干细胞蛋白）（抗原）        0.5mg/6501mg/1100             

QY-00080    Nestin巢蛋白（神经上皮干细胞蛋白）（抗原）        0.5mg/6501mg/1100             

QY-02890    neurofasciu-155神经束蛋白-155        0.5mg/6501mg/1100             

QY-00670    NGF-β神经生长因子-β(多肽片断抗原)      0.5mg/6501mg/1100             

QY-01610    NGF-R(p75NTR)神经生长因子受体（抗原）        0.5mg/6501mg/1100             

QY-00740    NIK，NF-kappaB-Inducing KinaseNFkB诱导的激酶（抗原）         0.5mg/6501mg/1100             

QY-05170    ATF1 (Activating Transcription Factor1)活化复制因子1         0.5mg/650             

QY-00700    NKB （Neurokinins B）神经激肽B（抗原）       0.5mg/6501mg/1100