大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家

齐一生物试剂盒组分经过重重优化，每个组分的稳定性都完美的经过高温破坏测验，最大程度的避免了长途运输和保存过程中抗原抗体的失活.齐一生物试剂盒凭借其完美的标曲.严格的质控.稳定高、易保存，操作简便，已广泛应用在全国免疫学检验研究所的各领域中.深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】齐一生物试剂盒凭借其完美的标曲、严格的质控、较高的稳定性，在世界范围内的科研试剂领域中脱颖而出深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

操作步骤
实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。
1.         加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。
为保证实验结果有效性，每次实验请使用新的标准品溶液。
2.         弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。
3.         温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，350μl/每孔，甩干。
4.         每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。
5.         温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔梯度不明显，即可终止）。
7.         依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。
8.         用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。
【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】注：
1. 用户在初次使用试剂盒时，应将各种试剂管离心数分钟，以便试剂集中到管底。
2. 每次实验留一孔作为空白调零孔，该孔不加任何试剂，只是最后加底物溶液及2N H2SO4。测量时先用此孔调OD值至零。
3. 为防止样品蒸发，试验时将反应板放于铺有湿布的密闭盒内，酶标板加上盖或覆膜。
4. 未使用完的酶标板或者试剂，请于2-8℃保存。标准品、生物素标记抗体工作液、辣根过氧化物酶标记亲和素工作液请依据所需的量配置使用。请勿重复使用已稀释过的标准品、生物素标记抗体工作液或、辣根过氧化物酶标记亲和素工作液。
5. 建议检测样品时均设双孔测定，以保证检测结果的准确性。
以上资料仅供您参考，如有问题欢迎来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

764114  PAXgene Bone Marrow RNA Tubes (50)      CNY             【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】

679913  ipsogen RT Kit (33)     CNY            

203643  HotStarTaq Plus Master Mix Kit (250)    "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."   CNY            

201225  Taq PCR Core Kit (1000) "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."    CNY            

201203  Taq DNA Polymerase (250)    "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."    CNY            

180922  GeneRead Adapter L Set 1plex (12)       CNY            

180912  GeneRead Adapter I Set 1plex (12)       CNY            

159214  QIAcard FTA Indicator Four Spots (25)       CNY            

159201  QIAcard FTA One Spot (100)      CNY            

9235523 Needle cleaning assembly P/N 4567-01        CNY            

9235300 Plunger tip, syringe 2,5ml      CNY            

9235117 Fuse F4A, v1.00     CNY            

9022818 EZ1 Advanced XL Flip Cap Tube Rack      CNY            

9234889 Vacuum manifold, 96 seal        CNY            

9234862 System liquid container (2l Nalgene)        CNY            

9239555 PCBA, Laser Driver, Unlimited       CNY            

9238664 Hinge       CNY             【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】

9238662 Lid with window     CNY            

9238659 Cooling compressor T2168GK, 60Hz        CNY            

9238645 Brake resistor 112 Ohm / 100 W      CNY            

9242061 Pipetting container, IQ/OQ, QS      CNY            

9241827 Pyro Q96 ID, Premium Agreement  "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241166 Board, A/D, QX      CNY            

9240955 Sensor, z, handler, Qsym        CNY            

9240835 QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY            

9240766 32 OUT Module, D2-32TD1, AP96       CNY            

6000-5018   MST STM Rack & Lid (Blue - 2 pos. cal.)     CNY            

9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY            

9241375 Magnet, door lock, top, EZ1a        CNY            

9241334 Keypad foil, EZ1        CNY            

9240750 Belt, Elevator, AP      CNY            

9243648 Acceler. Valid Supp, Other Extract Sys  The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).    CNY            

QY-x1938    ZR-75-1 乳腺导管癌 SF767 脑瘤                       【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】

QY-x1939    A10大鼠主动脉平滑肌                        

QY-x1940    人主动脉平滑肌　CNE-1 高分化鼻咽鳞癌 1640+10%胎牛血清                      

QY-x1941    CNE-2 低分化鼻咽鳞癌 1640+10%胎牛血清                      

QY-x1942    KB 口腔表皮样癌 1640+10%胎牛血清                       

QY-x1943    Hep-2 喉癌 1640+10%胎牛血清                    

QY-x1944    Ec109 食管癌 1640+10%胎牛血清                      

QY-x1945    KYSE 450 食管癌 1640+10%胎牛血清                       

QY-x1946    KYSE 150 食管鳞癌 1640+10%胎牛血清                     

QY-x1947    EC97076 食管癌 1640+10%胎牛血清                    

QY-x1948    BGC-823 胃腺癌 1640+10%胎牛血清                    

QY-x1949    MGC-803 胃腺癌 1640+10%胎牛血清                    

QY-x1950    SGC7901 胃低分化腺癌 1640+10%胎牛血清(P53基因第六外显子204位密码发生缺失突变)                      

QY-x1951    MKN-45 低分化胃癌 1640+10%胎牛血清                     

QY-x1952    HuTu-80 十二指肠腺癌 MEM+非必需氨基酸+10%胎牛血清                      

QY-x1953    Caco-2 结肠癌 MEM+非必需氨基酸+10%胎牛血清                     

QY-x1954    COLO 205 结肠癌 1640+10%胎牛血清                        【大鼠前列腺素F2a（PGF2a）Elias试剂盒厂家】

QY-x1955    COLO320DM 结肠癌 1640+10%胎牛血清                      

QY-x1956    HCT-8 回盲肠腺癌 1640+10%胎牛血清                      

QY-x1957    HCT-116 低分化结肠腺癌 1640+10%胎牛血清                    

QY-x1958    HT-29 结肠腺癌 1640+10%胎牛血清                    

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QY-x1960    SW480 结肠癌 L15                       

QY-x1961    BEL-7402 肝细胞癌 1640+10%胎牛血清                     

QY-x1962    BEL-7405 肝细胞癌 1640+10%胎牛血清                     

QY-x1963    H7402 肝癌 1640+10%胎牛血清                    

QY-x1964    HCC-9204 肝癌 1640+10%胎牛血清                     

QY-x1965    Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                       

QY-x1966    Hep G2 肝癌 DMEM+10%胎牛血清                       

QY-x1967    SMMC-7721 肝癌 1640+10%胎牛血清                    

QY-x1968    PANC-1 胰腺导管上皮癌 1640+10%胎牛血清