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本试剂盒仅供研究使用,齐一生物销售:021-6034 8496;181214 53965;173021 04490欢迎选购!
操作步骤
1.准备试剂,样品和标准品
2.加入准备好的样品和标准品,37℃反应30分钟
3.洗板5次,加入酶标试剂,37℃反应30分钟
4.洗板5次,加入显色液A、B,37℃显色15分钟
5.加入终止液
6.15分钟之内读OD值
7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。
【大鼠白细胞介素5(IL-5)Elias试剂盒现货】样本保存条件:
1.2-8℃冰箱最多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
【大鼠白细胞介素5(IL-5)Elias试剂盒现货】样本收集
1.血清样本用干燥管(红色)或者促凝管(黄色)收集
2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
3.动物组织匀浆(细胞):
1g组织加入9ml PBS(浓度为0.1M,pH为7.4的)测分泌型指标
1g组织加入1ml组织裂解液加入8ml PBS 测细胞内
粪便样本:1g样本+9ml PBS(浓度为0.01M,pH为7.4的)
没有PBS的话也可以用一下生理盐水,最好还是用PBS
【大鼠白细胞介素5(IL-5)Elias试剂盒现货】样本离心转速及时间
1.试管采集的样本:3000转,离心五分钟取上清
2.离心管或者EP管采集的样本:5000--8000转3分钟取上清
3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
【大鼠白细胞介素5(IL-5)Elias试剂盒现货】以上样本采集及处理方式供您参考,如有问题请来电,齐一生物销售:021-6034 8496;181214 53965;173021 04490
9240523 Cable, Motor, Axis #0, Dispense, AP98 CNY 【大鼠白细胞介素5(IL-5)Elias试剂盒现货】
9240507 Software & Std. Protocols, AP196 CNY
9240377 QIAcube, Installation The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware and software installation of the QIAcube instrument and provides the user with an introductory training on operation and user maintenance. The installation includes labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY
9239571 BR Universal, Basic Agreement The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9239570 BR Universal, Premium Agreement The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY
9020032 FOOTSET, RGQ CNY
9019986 PCB, small breakout, 12 core, C12 CNY
9019479 UPPER BUFFER LID GS CNY
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9238852 LiquiChip, Basic Agreement The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9241224 QIAxcel, Inspection Service "Repair service delivery not applicable
Service response time (repair) not applicable
Periodic inspection/maintenance: yes, 1/agreement year
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: not included
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: Yes, included" CNY
9241197 Silicone sealant, RTV 108, QX CNY
9241183 Sensor, Y, with cable, QX CNY
9233329 Belt, tooth, X-drive, BR8000 CNY
9233299 Toothbelt, X-drive, P3xx1 CNY
9233276 Motor, bar-code camera, Y-motor CNY
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9237711 #BR M48, Installation CNY
9237164 Bezel XYP CNY
9237161 Adapter Vertical Height CNY
9237121 Upgrade App., R.E.A.L. Prep 96 CNY
9237112 Upgrade App., QIAamp 96 Swab Take advantage of our instrumentation and molecular biology expertise to install QIAamp 96 DNA Swab on your BioRobot System. The onsite protocol development visit includes all travel and labor for the visit to install the standard QIAGEN QIAamp 96 DNA Swab protocol. Also included is a QIAamp 96 DNA Swab BioRobot 9604 Kit (12-plates) SAP #965842 CNY
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9236355 Sample Needle, short CNY
9232936 Microplate Reader (UV-340nm)Module CNY
9232915 Washer Board CNY
9236325 Sample Valve CNY
9230821 Rack for reagents,Troughs(for 6 troughs) CNY
4823024 careHIV-1 RT-PCR ASSAY CNY
4861014 careEBV PCR ASSAY I CNY
130823 NeXtal CubicPhase 2w Mo/C 8% µplate (2) CNY
130822 NeXtal CubicPhase 1w Mo/C 8% µplate (10) CNY
NG0169 DNA产物纯化试剂盒 50T BR
NG0170 PCR纯化试剂盒 100T BR
NG0171 胶回收试剂盒 50T BR
NG0172 玻璃珠DNA胶回收试剂盒 50T BR
NG0173 EZSpin柱式DNA胶回收试剂盒/UNIQ-10柱式DNA胶回收试剂盒/EZSpinColumnDNAGelExtractionKit 50T BR 【大鼠白细胞介素5(IL-5)Elias试剂盒现货】
NG0174 EZSpin柱式PAGE胶DNA回收试剂盒/UNIQ-10柱式PAGE胶DNA回收试剂盒/EZSpinColumnPAGEGelDNAExtractionKit 50T BR
NG0175 EZSpin柱式PAGE胶OligoDNA回收试剂盒/UNIQ-10柱式PAGE胶OligoDNA回收试剂盒/EZSpinColumnPAGEOligoGelDNAExtractionKit 50T BR
NG0176 EZSpin柱式PCR产物纯化试剂盒/UNlQ-10柱式PCR产物纯化试剂盒/EZSpinColumnPCRProductPurificationKit 50T BR
NG0177 96孔PCR产物纯化试剂盒/96wellplatePCRProductPurificationKit 5T BR
NG0178 96孔DNA清洁试剂盒/96wellplateDNACleanupKit 5T BR
NG0179 玻璃珠PCR产物纯化试剂盒/GoldenBeadsPCRProductPurificationKit 50T BR
NG0180 EZSpin柱式质粒小量抽提试剂盒/UNlQ-10柱式质粒小量抽提试剂盒/EZSpinColumnPlasmidMini-PrepsKit 50T BR
NG0181 EZSpin柱式质粒中量抽提试剂盒/UNlQ-200柱式质粒中量抽提试剂盒/EZSpinColumnPlasmidMedi-PrepsKit 10T BR
NG0182 EZSpin柱式质粒大量抽提试剂盒/UNlQ-500柱式质粒大量抽提试剂盒/EZSpinColumnPlasmidMaxi-PrepsKit 10T BR
NG0183 96孔质粒DNA小量抽提纯化试剂盒/96wellplatePlasmidMini-PrepsKit 5T BR
NG0184 96孔质粒DNA小量制备试剂盒/96wellplatepreparationMini-PrepsKit 5T BR
NG0185 EZSpin柱式无内毒素质粒抽提试剂盒/UNlQ-500柱式无内毒素质粒抽提试剂盒/EZSpinColumnEndotoxin-freePlasmidPrepsKit 50T BR
NG0186 EZSpin柱式一步式裂解质粒快速抽提试剂盒/UNlQ-10柱式一步式裂解质粒快速抽提试剂盒/EZSpinColumnone-steplysisBacterialPlasmidRapidPrepsKit 50T BR
NG0187 EZSpin柱式酵母质粒抽提试剂盒/UNlQ-10柱式酵母质粒抽提试剂盒/EZSpinColumnYeastPlasmidPrepsKit 50T BR
NG0188 EZSpin柱式细菌基因组DNA抽提试剂盒/UNlQ-10柱式细菌基因组DNA抽提试剂盒/EZSpinColumnBacterialGenomicDNAIsolationKit 50T BR
NG0189 96孔细菌基因组DNA小量抽提试剂盒/96wellplateBacterialGenomicDNAMini-PrepsKit 5T BR 【大鼠白细胞介素5(IL-5)Elias试剂盒现货】
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NG0198 胃蛋白酶测试盒 50管/24样 比色法
NG0199 胰蛋白酶测试盒 50管/48样 比色法
NG0200 丙酮酸测试盒 50管/48样 比色法
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