大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9001903  QIAgility HEPA/UV (PC) Priority        QIAgility HEPA/UV (PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133122     NeXtalStock Potassium citrate (200)                 CNY                             

963141     QIAquick 96 PCR BioRobot Kit (4)    For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads  CNY                             

133193     NeXtalStock di-Potassium phosphate (200)               CNY                             

9018555  Cover, front, top, EZ1a              CNY                             

9018528  User Manual EZ1 Advanced              CNY                             

37601       Qproteome Plasma Membrane Protein Kit              CNY                             

9015329  O-rings, Norpren tube fixing, M48 (2)               CNY                             

9015328  Seal, cap, M48 (6)             CNY                             

239095     GelPilot 1 kb Plus Ladder (100)         Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp         CNY                             

133127     NeXtalStock Sodium citrate (200)             CNY                              【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】

133126     NeXtalStock TRIS-HCl pH 8.5 (200)           CNY                             

133008     NeXtalStock 2-Propanol (200)           CNY                             

133007     NeXtalStock MES sod. salt untit. (200)              CNY                             

9011949  Board, X-main           CNY                             

9011945  Tube Holder, STS, 11ml (6)       Holder for accommodating 8 tubes (11 ml) in the sample tracking system; pack of 6    CNY                             

9243588  RCS, Premium Agreement       "- Service response time RCS: 24 Hrs

- Inspection/maintenance delivery: yes, included; 1 /agreement year

- Cost coverage for repair parts: yes, included

- Cost coverage for labor: yes, included

- Cost coverage for travel: yes, included

- Replacement system provision for DML: yes, included

- Transportation cost coverage for DML: yes, included

For defect units, after customer calling QIAGEN technical service hotline before 9 am:

RCS:

- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 24 Hrs.

DML:

- A special package containing a replacement unit shall be sent to the customer within 48 Hrs / 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"    CNY                             

37013       Albumin Affinity Cartridges (3 x 1ml)                 CNY                             

36910       Single-Tube Magnet         Magnet for separating magnetic particles in a 1.5 ml or 2 ml tube         CNY                             

36111       Ni-NTA Magnetic Agarose Beads (2 x 1 ml)     2 x 1 ml nickel-charged magnetic agarose beads (5% suspension)  CNY                             

35330       Penta-His Alexa Fluor 532 Conjugate       125 ul Penta-His Alexa Fluor 532 Conjugate, 200 ug/ml CNY                             

218432     Mouse miScript Assay 384 Set VXX.X (20)                  CNY                             

218412     Mouse miScript Primer Assay Set VXX.X           CNY                             

218380     #miScript PCR Control Set        miScript PCR Control Set allows customers to identify easily the right normalization control for their real-time PCR set up while working with miRNAs or other small RNAs. It includes 6 normalization control assays (for 50 real-time PCR rxns a 50µl) which are all designed against snoRNAs or snRNAs. Works for human mouse ａnd rat.        CNY                             

218193     miScript PCR Starter Kit (80)             CNY                             

9018294  EZ1 Advanced DNA Buffy Coat Card                  CNY                             

800356     Handling Fee             CNY                              【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】

9015298  Nozzles, M48 (6)               CNY                             

9243775  QIAsymphony AS Custom Protocol Advanced2                  CNY                             

9019447  Sensor brd 4 CH temp sensor            CNY                             

9019444  Electrolytic capacitor 8000uF x 63V                  CNY                             

9018158  Waste station, waste drawer, Qsym                 CNY                             

9018154  Bottle, waste drawer, Qsym             CNY                             

QY-17752 anti-RSV（Mouse anti-respiratory syncytial virus Monoclonal）鼠抗呼吸道合胞病毒单克隆抗体                                                 

QY-17762 Anti-Runx3(CBFA3)新型抑癌基因/一种新发现的抑癌基因抗体                                              

QY-17772 Anti-S100BS100B蛋白抗体                                                  

QY-17782 anti-S-100A1 (S100 calcium-binding protein A1)钙结合蛋白S100A1抗体                                                 

QY-17792 Anti-SAP-1/FRG4(Synapse associated protein- I)突触相关蛋白-1抗体                                               

QY-17802 Anti-SARS putative orflab polyprotein （SARS coronavirus CUHK-W1）                                                     

QY-17812 Anti-SARS S-protein非典病毒表面蛋白S抗体                                                   

QY-17822 Anti-SCF (Stem Cell Factor)干细胞生长因子抗体                                                 【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】

QY-17832 Anti-SDF-1/CXCL12 (stromal cell derived factor-1)基质细胞衍生因子-1抗体                                                     

QY-17842 Anti-Secretin分泌素抗体                                                     

QY-17852 Anti-Secretin receptor分泌素受体抗体                                                      

QY-17862 Anti-Selenoprotein W硒蛋白W抗体                                                  

QY-17872 Anti-Sema3A (semaphorin 3A)臂板蛋白3A抗体                                                

QY-17882 Anti-Sema3F (semaphorin 3F)臂板蛋白3F抗体                                                  

QY-17892 Anti-Sema4C (semaphorin 4C)臂板蛋白4C抗体                                                 

QY-17902 anti-SFRP1（Secreted frizzled related protein 1）分泌型卷曲相关蛋白1抗体                                                       

QY-17912 Anti-SGC (soluble glycoprotein C)可溶性糖蛋白C抗体                                                       

QY-17922 Anti-SGLT1 (sodium-glucose cotransporter1)钠-葡萄糖共转运载体1抗体                                               

QY-17932 Anti-Shank1(SH3 ａnd multiple anleyrin repead domins protein 1)shank-1抗体                                                   

QY-17942 Anti-SHBG (Sex Hormone Binding Globulin)性激素结合球蛋白抗体                                                  

QY-17952 Shiga-like toxin IIe variant subunit ACAS:Escherichia coli抗猪水肿病大肠杆菌志贺样毒素Ⅱ型突变体（O139菌株）抗体                                                       

QY-17962 Anti-SHP-1 (Src homology phosphatase-1)造血细胞磷酸酶抗体                                                

QY-17972 Anti-SHP2/PTPN11（Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2）蛋白酪氨酸磷酸酶2抗体                                                

QY-17982 Anti-SIP1 (survival of motor neuron protein interacting protein 1)运动神经元存活蛋白结合蛋白1抗体                                                【大鼠肝细胞生长因子(HGF)ELISA试剂盒厂家】

QY-17992 Anti-Skp2 (S-phase kinase-associated protein 2,SKP2)细胞S相激酶相关蛋白抗体                                                   

QY-18002 Anti-Slc22A17 (solute carrier famili 22A17)可溶性载质转运蛋白22A17抗体                                                   

QY-18012 Anti-SLC24A5 (solute carrier family 24, member 5)可溶性载质转运蛋白SLC24A5抗体                                                   

QY-18022 Anti-SLC6A19 (Solute carrier family 6, member 19)钾依赖钠钙交换蛋白6                                               

QY-18032 anti-Smac/DIABLO（the second mitochondrial activator of caspase）线粒体促凋亡蛋白抗体                                              

QY-18042 SLC34A2/NaPi-2b(Solute carrier family 34 member 2)磷酸钠协同转运蛋白抗体                                                       

QY-18052 Anti-Smad2/3(Mothers against decapentaplegic homolog 2)Smad 2/3抗体