大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

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For quantitative, real-time PCR ａnd two-step RT-PCR using sequence-specific probes."         CNY                              【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】

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9015332  Sensor, pressure, M48               CNY                             

9015324  Scrapers, M48 (6)              CNY                             

133028     NeXtalStock CAPSO untit. (200)                 CNY                             

133027     NeXtalStock CAPS untit. (200)          CNY                             

129112     RNase-Free Water (12 x 1,9 ml)                CNY                             

81031       Plate Rotor 2 x 96    Rotor for 2 QIAGEN 96-well plates, for use with QIAGEN Centrifuges    CNY                             

80234       Allprep FFPE DNA/ RNA Kit (50)                CNY                             

74134       RNeasy Plus Mini Kit (50)          50 RNeasy Mini Spin Columns, 50 gDNA Eliminator Mini Spin Columns, Collection Tubes Rnase Free  Reagents ａnd Buffers   CNY                             

27106       QIAprep Spin Miniprep Kit (250)      For 250 high-purity plasmid minipreps: 250 QIAprep Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

20021       QIAEX II Gel Extraction Kit (150)     For 150 extractions: 3 x 0.5 ml QIAEX II Suspension, Buffers  CNY                             

69990       Stainless Steel Beads, 7mm               CNY                             

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19090       Buffer RPW (125ml)                   CNY                             

19082       Buffer ASL (4 x 140 ml)     560 ml Stool Lysis Buffer  CNY                             

19065       Buffer PE (concentrate)   100 ml Wash Buffer (5x concentrate for 500 ml buffer)  CNY                             

19052       Buffer P2 500 ml Lysis Buffer   CNY                             

12663       HiSpeed Plasmid Maxi Kit (25)          25 HiSpeed Maxi Tips, 25 QIAfilter Maxi Cartridges, 25 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers      CNY                             

12245       QIAfilter Plasmid Midi Kit (100)        100 QIAGEN-tip 100, Reagents, Buffers, 100 QIAfilter Midi Cartridges CNY                            

12143       QIAGEN Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers    CNY                             

11500       Ultrapure 500 (1)              CNY                             

10083       QIAGEN-tip 2500 (25)      25 columns; 2.5 mg column capacity       CNY                             

133268     NeXtalStock Pentaerythritol ethoxy.(200)                 CNY                             

990602     Elution Plate (10)               CNY                             

990542     200µl Filtered Sterile Tips (960)                CNY                             

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949020     Autopure Qubes E (192)            CNY                             

990391     Rack Labeling Strips                   CNY                             

QY-11862 Anti-CRF (Corticotropin-Releasing Factor)促肾上腺皮质激素释放因子                                                     

QY-11872 anti-CSP (circumsporozoite protein/Plasmodium yoelii)环子孢子蛋白(约氏疟原虫)抗体                                                 

QY-11882 Anti-CT （Calcitonin )降钙素抗体                                              

QY-11892 Anti-CT DNA (culf thymus DNA)兔抗小牛胸腺DNA 抗体                                                  

QY-11902 Anti-CTGF (connective tissue growth factor)结缔组织生长因子抗体                                                 【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】

QY-11912 Anti-CTLA-4/CD152（cytotoxic T-lymphocyte associated antigen-4）淋巴细胞相关抗原4/细胞毒性T细胞抗原-4抗体                                                    

QY-11922 anti-CTn1 (cardiac troponin 1)心肌肌钙蛋白抗体                                              

QY-11932 Anti-CT-R（Calcitonin receptor）降钙素受体抗体                                                     

QY-11942 Anti-CULLIN/Cdc53/CUL/SCFCdc4CULLIN抗体                                                      

QY-11952 Anti-Cx40 (Connexin-40)间隙连接蛋白40抗体                                                  

QY-11962 Anti-Cx43 (Connexin-43)间隙连接蛋白43抗体                                                  

QY-11972 Anti-CX45 (Connexin-45)间隙连接蛋白45抗体                                                  

QY-11982 Anti-CXC-R1 (CXC-chemokine receptor 1)细胞表面趋化因子受体1抗体                                                  

QY-11992 Anti-CXC-R3/CD183 (Chemokine receptorC-X-C chemokine receptor type 3)细胞表面趋化因子受体3抗体                                                     

QY-12002 anti-cx3cl1(chemokine (C-X3-C motif) ligand 1)趋化因子(C-X3-C 基元)配体1抗体                                                   

QY-12012 Anti-CXorf36 (uncharacterized protein Cxorf36 precursor)脱羧酶蛋白体3抗体                                              

QY-12022 Anti-CXorf36 (uncharacterized protein Cxorf36 precursor)脱羧酶蛋白体36抗体                                                     

QY-12032 Anti-Cyclin A周期素A抗体                                                 

QY-12042 Anti-Cyclin B1周期素B1抗体                                                      

QY-12052 Anti-Cyclin C周期素C抗体                                                  

QY-12062 Anti-Cyclin D1周期素D1抗体                                                      【大鼠γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒厂家】

QY-12072 Anti-Cyclin D2周期素D2抗体                                                     

QY-12082 Anti-Cyclin E周期素E/原癌基因抗体                                                 

QY-12092 Anti-Cyclin H周期素H抗体                                                 

QY-12102 Anti-Cygb (cytoglobin)细胞球蛋白抗体                                               

QY-12112 Anti-CYP450(Cytochrome P450 monooxygenase)细胞色素P450单氧化酶抗体                                               

QY-12122 anti-Cyr61（cysteine rich 61）富半胱氨酸肝素结合蛋白61                                                    

QY-12132 anti-CYP19细胞色素P450 19抗体                                                      

QY-12142 Anti-Cytochrome C（Cyt C）细胞色素 C抗体                                                    

QY-12152 Anti-Cytokeratin（CT）细胞角蛋白抗体（C端）                                                      

QY-12162 Anti-anti-HCMV PP65/HHV5 PP65(Human Cytomegalovirus PP65 ) 人巨细胞病毒PP65/CMV低基质磷脂蛋白抗体