大鼠醛固酮(ALD)ELISA试剂盒现货

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【大鼠醛固酮(ALD)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【大鼠醛固酮(ALD)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【大鼠醛固酮(ALD)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【大鼠醛固酮(ALD)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9012727  Syringe pump inlet tubing 3002                 CNY                             

978801     PyroMark OneStep RT-PCR Kit (50)          CNY                             

978703     PyroMark PCR Kit (200)            CNY                             

9012715  Troughs, 80ml (100 troughs + lids)            CNY                             

133241     NeXtalStock Sodium malate pH 7.0 (200)                  CNY                             

133232     NeXtalStock Sodium cacodyl. pH 7.0 (100)                CNY                              【大鼠醛固酮(ALD)ELISA试剂盒现货】

57414       QIAamp MinElute Media Kit (50)              CNY                             

973043     PyroMark Q96 CpG LINE-1 (192)      For quantification of global methylation level in transposable elements         CNY                             

972812     PyroMark Gold Q96 SQA Reagents (1 x 96)     "For performing pyrosequencing reaction on the Q96 ID (1x96) 

SQA= Sequencing Analysis"      CNY                             

972432     PyroMark Q96 SNP MTHFR (96)       For detection of variants in MTHFR gene         CNY                             

133223     NeXtalStock Sodium ac. pH 4.2 (200)                CNY                             

133216     NeXtalStock MOPS pH 6.5 (200)                CNY                             

9002032  Rotor-Gene Q MDx 5plex HRM Platform                  CNY                             

9002028  Rotor-Gene Q MDx 5plex, Priority  Rotor-Gene Q 6plex (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9002023  Rotor-Gene Q MDx 5plex System             CNY                             

9002021  Rotor-Gene Q MDx 5Plex no PC                CNY                             

970042     PyroMark Q24 CpG LINE-1 (4 x 24)  For quantification of global methylation level in transposable elements         CNY                             

133206     NeXtalStock Potassium formate (200)              CNY                             

929523     QX Alignment Marker 15 bp/10 kb (1.5 ml)               CNY                             

929500     QX Intensity Calibration Marker (1.0 ml)                   CNY                             

9012623  Pippeting Station, 3 microtitre plates               CNY                             

291073     mericon Mon 810 Corn (24)              CNY                              【大鼠醛固酮(ALD)ELISA试剂盒现货】

9001917  PyroMark Q96 MD, Priority     PyroMark Q96 MD, Priority includes the PyroMark Q96 MD instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133113     NeXtalStock Sodium Iodide (200)              CNY                             

967152     RNeasy 96 BioRobot 8000 Kit (12)  For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 8000: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd Buffers         CNY                             

9018464  Printer, EZ1a             CNY                             

9018543  Anti-corrosion oil (rotor), QIAC                  CNY                             

9018466  Transport Box, EZ1 Advanced           CNY                             

9001881  QIAsymphony SP, PrioPLUS       "QIAsymphony SP  Instrument

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955235     MagAttract Viral RNA M48 Kit (96)         For 96 viral RNA preps: MagAttract Suspension F, Buffers      CNY                             

9015456  Board, T-net main             CNY                             

9015437  CD, Manual, Service, BIOROBOT MDx             CNY                             

9016646  Tubing, syringe outlet, BR8 UNIV             CNY                             

QY-06890 PEPT1 (Peptide-transporters 1)肠道肽转运蛋白(小肽转运蛋白)多肽                    0.5mg/6501mg/1100                                  

QY-07480 PTEN/MMAC1一种肿瘤抑制基因抗原                    0.5mg/650                                  

QY-04200 PIBF（progesterone induced blocking factor）孕激素诱导阻断因子(抗原)                  0.5mg/6501mg/1100                                 

QY-0665R          PIWIL1(piwi-like 1)piwi 样1蛋白(抗原)                   0.5mg/6501mg/1100                                  

QY-05300 PPARγ (peroxisome proliferator-activated receptor-gamma)过氧化酶活化增生受体γ（抗原）           0.5mg/6501mg/1100                                  

QY-04900 PP2Ac蛋白磷酸酶4(抗原)               0.5mg/6501mg/1100                                  

QY-03780 Runx3一种新发现的抑癌基因(抗原)              0.5mg/6501mg/1100                                  

QY-02780 RRAD (Ras-related associated with diabetes )RRAD(多肽抗原)               0.5mg/6501mg/1100                                  

QY-07830 SDF-1/CXCL12 (stromal cell derived factor-1)基质细胞衍生因子-1(抗原)                0.5mg/6501mg/1100                                  

QY-02110 shank1(SH3 ａnd multiple anleyrin repead domins protein 1)shank1抗原                 0.5mg/6501mg/1100                                  

QY-04440 slc22A17 (solute carrier famili 22)溶质转运蛋白家族22成员17(多肽抗原)                 0.5mg/6501mg/1100                                 

QY-04960 SLC24A5 (solute carrier family 24, member 5)可溶性载质转运蛋白SLC24A5（抗原）                0.5mg/6501mg/1100                                  

QY-04730 solute carrier family 1溶质携带物家族-1(抗原)              0.5mg/6501mg/1100                                  【大鼠醛固酮(ALD)ELISA试剂盒现货】

QY-04780 SLC6A4 (solute carrier family 6)钾依赖钠钙交换蛋白6(抗原)                0.5mg/6501mg/1100                                  

QY-06150 Survivin细胞凋亡抑制因子的一种（抗原）                  0.5mg/6501mg/1100                                  

QY-07260 BIRC5/Survivin variant细胞凋亡抑制因子4（抗原）            0.5mg/6501mg/1100                                  

QY-06350 SSA/RO (ribonucleoprotein complex,RNP)核糖核蛋白抗原SSA(抗原)            0.5ml/6501mg/1100                                   

QY-02760 SSB/La(Sjogren syndrome type B antigen)(抗原)抗干燥症SSB/La蛋白抗体                   0.5mg/6501mg/1100                                 

QY-07180 Smad2(Mothers against decapentaplegic homolog 2)Smad 2/3(抗原)              0.5mg/6501mg/1100                                  

QY-07170 SPAG5/map126 (sperm associated antigen 5）抗精子相关抗原5(抗原)                0.2ml/1280                                 

QY-07700 SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)                   0.5mg/650                                  

QY-07700 SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)                   0.5mg/6501mg/1100                                  

QY-06790 SYVN1(synovial apoptosis inhibitor 1)滑膜细胞凋亡抑制物1（抗体）                  0.5mg/6501mg/1100                                  

QY-06850 SYK (Spleen tyrosine kinase)非受体型酪氨酸激酶(抗原)                 0.5mg/6501mg/1100                                  

QY-04190 Tau protein微管相关蛋白（抗原）                0.5mg/6501mg/1100                                  

QY-06380 TGF-βR 1/ALK-5(TGF-beta receptor type I)转移生长因子–β受体 1（抗原）                   0.5mg/6501mg/1100                                  

QY-07780 Thy-1/CD90CD90(抗原)              0.5mg/6501mg/1100                                  

QY-06320 Thioster-containing protein 1硫酯包含蛋白-1(抗原)              0.5mg/6501mg/1100                                  

QY-06400 TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )P53诱导糖酵解和凋亡调节因子蛋白（抗原）            0.5mg/6501mg/1100                                   【大鼠醛固酮(ALD)ELISA试剂盒现货】

QY-04180 TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金属蛋白酶组织抑制因子-4(抗原)             0.5mg/6501mg/1100                                  

QY-04470 TPO(Thyroid Peroxidase)甲状腺过氧化物酶(抗原)                  0.5mg/6501mg/1100