大鼠去甲肾上腺素(NA)ELISA试剂盒厂家

价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供最优质产品。

【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间最好控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，最好做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请最后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9016612  Documentation, IQ/OQ, BR MDx DSP              CNY                             

9016572  Tip Wash, RCS1                  CNY                             

9016519  Round Carrier Set (4), 4 x 50ml Falcon              CNY                             

9016511  Rect. Bucket Set (6)                   CNY                             

9016241  QIAsoft M48, Directory Upgr. Tool           CNY                              【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

9016187  EZ1 Test Card            CNY                             

9016147  #App. Package, M48, Genetic Screening                  CNY                             

9016145  #App. Package, M48, Inf. Dis. (CD)           CNY                             

9016121  Maintenance Kit, RoboVac, BR8000 II,SBS               CNY                             

9019171  Heating block, PyroQ96MD              CNY                             

9011915  Tubing set,  BR96OO, 1.02              CNY                             

9011892  Tubing, teflon, M6, 2.5 x 0.5 mm, 0.65 m                 CNY                             

9011887  Holder, tip rack, front unit                 CNY                             

9011881  Front unit, BR9600           CNY                             

9011878  Shaker adapter, microplate     Adapter for accommodating four 96-well plates or othe labware with the same base dimensions) on the 4-plate shaker system; not for use with the robotic handling system       CNY                             

9011862  Worktable right, BR8000 v1.01                 CNY                             

9019147  IPC Hard Drive - no software, PyroQ96             CNY                             

9019072  PyroMark Plate Hotel, PyroQ96MD                  CNY                             

9019062  PyroMark Q24 Software  For use with PyroMark Q24 instrument.Software for setting up a run ａnd analyzing mutations ａnd CpGs         CNY                             

9019000  Cover, rear, EZ1xl              CNY                             

9018998  Cover, front, top, EZ1xl             CNY                              【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

9018956  Reagent Block, 24x2ml Flat-Base Tubes           CNY                            

9018945  Adapter QIAGEN BioRobot M48 Tubes             CNY                             

9022706  Clevis Pin, X-pulley, DML3000           CNY                             

9016062  Tubing Set, Wash pump, BR8000 I            CNY                             

9016031  Tubing, retractable, DISU, BR8 RD v2              CNY                             

9015712  DISU Retraction Mechanism BR8,complete             CNY                             

9015654  Communication cable RS485, BR M48              CNY                             

9015594  PM Kit Biorobot EZ1                  CNY                             

9019160  Valve - Dispense, PyroQ96MD          CNY                             

9014206  Tubing Set, Waste Sys., BR8 RD                CNY                             

9014139  Sensor; 4l-Bottle; Vac.-Cont.; BR8000               CNY                             

9014118  Tubing, vacuum, textile reinforced, 3m            CNY                             

QY-04600 TSHR (Thyroid stimulating hormone receptor)促甲状腺素受体（抗原）              0.5mg/6501mg/1100                                  

QY-04490 TSLP(Thymic stromal lymphopoietin isoform 1)淋巴细胞生成素-1（抗原）                   0.5mg/6501mg/1100                                 

QY-04460 TTF-2(thyroid transcription factor 2;forkhead box E1)甲状腺转录因子-2(抗原)             0.5ml/6501mg/1100                                   

QY-08190 Tyrosinase酪氨酸酶(多肽抗原)               0.5mg/6501mg/1100                                  

QY-06030 OAT4L又称URAT1 (Urate Transporter 1)尿酸盐重吸收转运子1 (抗原)               0.5mg/6501mg/1100                                  

QY-08530 VAP1(vascular adhesion protein 1)血管粘附蛋白1（多肽抗原）                  0.5mg/6501mg/1100                                  

QY-06590 VCAM-1(Vascuolar cell adhesion molecule 1)CD106血管内皮细胞粘附分子(抗原)               0.5mg/6501mg/1100                                  

QY-07560 Vimentin波形蛋白（抗原）                    0.5mg/6501mg/1100                                  

QY-04790 VSIG4 (V-set ａnd immunoglobulin domain-containing protein 4)VSIG4 T淋巴细胞负调节蛋白之一（抗原）            0.5mg/6501mg/1100                                  

QY-04290 WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4)一种新的丝氨酸/苏氨酸激酶家族成员的基因WNK4（多肽抗原）                0.5mg/6501mg/1100                                  

QY-06280 ZNF300 (zinc finger protein ZNF300)- middle锌指蛋白300(抗原)                   0.5ml/6501mg/1100                                   

QY-0625R          ZNF268 (zinc finger protein ZNF268)- N-terminal (1a)锌指脂蛋白-1a(抗原)                    0.5mg/6501mg/1100                                  

QY-06260 ZNF268 (zinc finger protein ZNF268)- middle (1b)锌指脂蛋白-1b(抗原）               0.5mg/6501mg/1100                                  

QY-06270 ZNF268 (zinc finger protein ZNF268)- middle (1c)人锌指脂蛋白-1c(抗原)              0.5mg/6501mg/1100                                  【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

QY-04800 IFN-γ(Interferon γ)Ag-mouse、rat干扰素-γ多肽抗原（大、小鼠）            0.5mg/6501mg/1100                                  

QY-04810 IFN-γ(Interferon γ)Ag-Human干扰素-γ多肽抗原（人）                 0.5mg/6501mg/1100                                  

QY-07840 IFN-β (Interferon β)Anti-mouse,rat干扰素-β抗原                    0.5mg/6501mg/1100                                  

QY-07870 IFN-β (Interferon β)Anti-human干扰素-β抗原                 0.5mg/6501mg/1100                                  

QY-06690 PSCA (Prostate Stem Cell Antigen) 前列腺干细胞抗原                    0.5mg/6501mg/1100                                  

QY-06450 sIgA大鼠分泌型IgA(抗原)               0.5mg/6501mg/1100                                  

QY-09040 Melamine/KLH三聚氰胺与血蓝蛋白偶联物                   5mg/200010mg/3000                                 

QY-09041 Melamine/RCA三聚氰胺与牛血清白蛋白偶联物                  2mg/7805mg/150010mg/2500                                   

QY-09042 Rabbit IgG Sepharose 4B兔IgG亲和层析柱                    2ml柱床体积/15005ml柱床体积/2500                                    

QY-09043 Mouse IgG Sepharose 4B小鼠IgG亲和层析柱               2ml柱床体积/20005ml柱床体积/3500                                    

QY-09044 rec Protein G-Sepharose 4B 重组蛋白G亲和层析柱            2ml柱床体积/20005ml柱床体积/4000                                    

QY-09045 rec Protein A-Sepharose 4B重组蛋白A亲和层析柱              2ml柱床体积 /15005ml柱床体积/2500                                 

QY-09046 rh-TNF-α(rh-Tumor Necrosis Factor-α Humen)重组人肿瘤坏死因子                  100ug/1200250ug/2100500ug/3800                                  

QY-09047 rHIL-1Ra（Recombinant Human Interleukin-1 receptor antagonist His Tag）重组人白介素-1受体拮抗剂                100ug/1200500ug/3800                            

QY-09048 rhBMP-2/BMP2(bone morphogenetic protein-2,BMP-2;rhBMP-2)重组人骨形态发生蛋白-2                500ug/5800                                

QY-09049 rhBMP-7/BMP2(bone morphogenetic protein-7,BMP-7;rhBMP-7)重组人骨形态发生蛋白-7                 500ug/6800                                 【大鼠去甲肾上腺素(NA)ELISA试剂盒厂家】

QY-09053 Melamine/OVA(ovalbumin)三聚氰胺与鸡卵白蛋白偶联物           10mg/1500