大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱最多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，最好还是用PBS

【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-x185  WEHI 22.1B淋巴细胞         TIB-54                               

QY-x186  NIH/3T3胚胎成纤维细胞    CRL-1658                                 

QY-x187  MC3T3-E1 Subclone 14小鼠原成骨细胞   CRL-2594                                 

QY-x188  L6大鼠成肌细胞         CRL-1458                                 

QY-x189  H9c22-1大鼠心肌细胞        CRL-1446                                 

QY-x190  RSC96大鼠雪旺细胞   CRL-2765                                  【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】

QY-x191  CHO/dhFr-中国仓鼠二氢叶酸还原酶缺陷细胞  CRL-9096                                 

QY-x192  Lec1卵巢细胞      CRL-1735                                 

QY-x193  293T/17 [HEK 293T/17] 人胚肾细胞  CRL-11268                                

QY-x194  AAV-293 胚肾细胞                                        

QY-x195  hFOB 1.19SV40 转染成骨细胞   CRL-11372                                

QY-x196  CCD-1095Sk皮肤细胞        CRL-2122                                 

QY-x197  Hs 578Bst乳腺细胞      HTB-125                                   

QY-x198  NK-92人恶性非霍奇金淋巴瘤患者的自然杀伤细胞       CRL-2407                                 

QY-x199  NK-92MI人恶性非霍奇金淋巴瘤患者的自然杀伤细胞   CRL-2408                                 

QY-x200  HMy2.CIR人B 淋巴母细胞       CRL-1993                                 

QY-x201  OK负鼠opossum肾细胞     CRL-1840                                 

QY-x202  FO骨髓瘤细胞     CRL-1646                                 

QY-x203  RAW 264.7单核巨噬细胞白血病       TIB-71                               

QY-x204  P19畸胎癌细胞    CRL-1825                                 

QY-x205  C6胶质瘤细胞      CCL-107                                   

QY-x206  RBL-2H3白血病细胞    CRL-2256                                  【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】

QY-x207  SiHa子宫颈癌细胞       HTB-35                              

QY-x208  MG-63骨肉瘤细胞       CRL-1427                                 

QY-x209  HEC-1-B子宫内膜腺癌细胞        HTB-113                                   

QY-x210  SW1353骨肉瘤细胞     HTB-94                              

QY-x211  ES-2卵巢透明细胞癌细胞   CRL-1978                                 

QY-x212  Saos-2成骨肉瘤细胞    HTB-85                              

QY-x213  MDA-MB-468乳腺癌细胞   HTB-132                                   

QY-x214  SK-MES-1肺鳞癌细胞        HTB-58                              

QY-x215  MDA-MB-435S乳腺癌细胞        HTB-129                                   

9020997  ZS Pipetting Module, DCU           CNY                           

9020909  Cable, UV curly cord, SMC, QGX              CNY                            【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】

9020908  Hinge, lid, SMC, QGX          CNY                           

9020643  Waste chute, straight, AS, Cabinet Qsym            CNY                           

9020632  Kit, hinges (4), Cabinet Qsym              CNY                           

9020629  Door, waste, SP, Cabinet Qsym           CNY                           

9020625  Kit, repair, Cabinet Qsym             CNY                           

9020332  EPROM - Motherboard, RGQ              CNY                           

9020233  Foot assembly, rear, CAS             CNY                           

9020158  Insulation disc - chamber bottom, RG3        CNY                           

9020096  Locking Pin, TLyser 2          CNY                           

9020085  Thrust Washer, TLyser         CNY                           

9239498  V-Housing, LX100         CNY                           

9239490  EX Laser Driver, Uniphase           CNY                           

9239390  PCR Plate Adapter, M96        CNY                           

9236510  BR 3000, PM Service    The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed. CNY                           

9232764  Disposable Troughs, 20 / 30 ml (10)    Troughs holding up to 20 ml of liquid; pack of 10       CNY                           

9236472  BR 9600, Full Agreement       The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                            【大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒现货】

9236443  BR, Installation       The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot Universal instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9239155  Assembly, vertical axis, TWI        CNY                           

9239108  Assy, Sample Needle, Actuator linkage         CNY                           

9232693  MTP-Plate Holder  2.01              CNY                           

9232629  Bottle, 500-ml, yellow fitting         CNY                           

9232602  Vacuum manifold, plate holder bottomless           CNY                           

9232579  Cooling block, 1,5ml             CNY                           

9238951  Locking lever, BS15              CNY                           

9238798  Rectangular bucket, sealable with cap          CNY                           

9238794  Angle rotor 6 x 250 ml          CNY                           

9016913  Tubing, press relief valve inlet DISU v2              CNY                           

9238942  Keyboard-Display assembly, BS15        CNY                           

9238753  Screwdriver, hex2mm, z-drive BR8             CNY                           

9238916  Power supply 40W, BS96             CNY                           

9238748  Coupling, CPC, screw, male, V2          CNY