大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-x1628 狗肾细胞隆突型MDCK superdome                                                     

QY-x1629 绿色荧光蛋白标记小鼠前胃癌细胞MFC-GFP                                                   

QY-x1630 小鼠肾足细胞Mouse podocyte                                                   

QY-x1631 小鼠子宫颈癌细胞U14                                              

QY-x1632 绿色荧光蛋白标记小鼠子宫颈癌细胞U14-GFP                                               

QY-x1633 小鼠浆细胞瘤MPC-11                                                 

QY-x1634 转PYTL基因小鼠睾丸支持细胞15P-1                                               【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】

QY-x1635 BALB/C小鼠肝上皮细胞BNL1ME A.7R.1                                                   

QY-x1636 小鼠结肠癌细胞CT26.WT [CT26WT]                                                   

QY-x1637 小鼠网织细胞肉瘤M5076                                                  

QY-x1638 小鼠正常肝细胞NCTC1469                                                 

QY-x1639 正常小鼠睾丸Leydig细胞TM3                                                  

QY-x1640 正常小鼠睾丸Leydig细胞TM4                                                  

QY-x1641 615小鼠乳腺癌瘤株Ca759                                                

QY-x1642 615小鼠乳腺癌瘤株Ca761                                                

QY-x1643 615小鼠乳腺癌瘤株Ca763                                                

QY-x1644 615小鼠T细胞性白血病瘤株L7712                                                 

QY-x1645 615小鼠T细胞性白血病瘤株L7912                                                 

QY-x1646 615小鼠肺癌瘤株HP615                                                    

QY-x1647 615小鼠滑膜肉瘤瘤株ZM755                                                   

QY-x1648 615小鼠乳头状肺腺癌瘤株P615                                                       

QY-x1649 KM小鼠子宫颈癌瘤株U27                                                

QY-x1650 KM小鼠网织细胞肉瘤瘤株LⅡ                                                  【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】

QY-x1651 KM小鼠脑神经胶质母细胞瘤瘤株G422                                                   

QY-x1652 615小鼠网织细胞性白血病瘤株L615                                              

QY-x1653 Walker氏癌肉瘤256瘤株W256                                                

QY-x1654 C57小鼠黑色素瘤瘤株ME                                                 

QY-x1655 KM小鼠未分化神经胶质瘤瘤株B22                                                 

QY-x1656 小鼠Lewis肺癌瘤株LLT                                                      

QY-x1657 615小鼠前胃癌瘤株Fc                                               

QY-x1658 小鼠乳腺癌细胞株EMT-6                                                   

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                             【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

9236166  Safety shield, RapidPlate          CNY                             

9230863  Anpressplatte Rose-80              CNY                             

9235538  Teaching Tool            CNY                             

9230282  Cable, RS323, robot to syringe pump, 225                CNY                             

5050-2002        hc2 Software V2 Package                  CNY                             

BS96RNA BioSprint 96 to RNA (384) - UDP               CNY                              【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】

9240730  Tubing, 1/4" OD, Red, AP          CNY                             

9240713  Poly Muffler Kit, AP96/196                 CNY                             

9240690  Bearings, Lead Screws, AP                CNY                             

9243654  Comprehen Valid., Other Quant. Sys.      """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

6000-5013        LumiCheck Plate User Package                  CNY                             

288103     bactotype Mycoplasma Mg/Ms PCR Kit (24)            CNY                             

1048147  QIAamp Viral RNA Mini Accessory Set              CNY                             

270043     cattletype BHV1 gB Ab (5)                CNY                             

1019992  Buffer Set (RNeasy 96 BioRobot 9604 Kit                  CNY                             

273401     pigtype Toxoplasma Ab (1)                CNY                             

273505     pigtype Trichinella Ab (20)                 CNY                             

9019538  ZLVBelting, flat cogged, 6T, 2.5mm, CASx                CNY                             

9020591  Tray Assembly, DML 3000                  CNY                             

9021007  Motor, Pipettor X, DCU             CNY                             

9013373  Fix kit, liquid handling, BR8000                 CNY                             

9013315  Motor, handler T/L, BR3000             CNY                             【大鼠水通道蛋白2(AQP-2)ELISA试剂盒厂家】