人血管生成素4(ANG-4)ELISA试剂盒现货

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【人血管生成素4(ANG-4)ELISA试剂盒现货】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人血管生成素4(ANG-4)ELISA试剂盒现货】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱最多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人血管生成素4(ANG-4)ELISA试剂盒现货】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与第一次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【人血管生成素4(ANG-4)ELISA试剂盒现货】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

79523       Small Spin Columns  24 RNase-free spin columns for Oligotex spin procedures       CNY                             

76544       RNAprotect Animal Blood Tubes 100µl (50)              CNY                             

74324       RNeasy Protect Saliva Mini Kit (50) "The RNeasy Protect Saliva Kit is a D-BOM: RNAprotect Saliva Reagent (50ml) #1037475

RNeasy Micro kit (50)  #74004."   CNY                             

19590       InhibitEX (100)          CNY                             

70042       Oligotex mRNA Midi Kit  For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers   CNY                             

69984       TissueLyser Adapter Set (2 x 96)      2 sets of Adapter Plates for use with Collection Microtubes (racked) on the TissueLyser         CNY                             

69582       DNeasy 96 Blood & Tissue Kit (12)  For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

19581       96-Well Microplates (24) 96-well microplates with round-bottom wells plus lids, 24 per case, for use with QIAvac manifolds ａnd the BioRobot 9600    CNY                             

19418       QIAvac Holder Kit             CNY                             

19408       VacValves (24) For processing QIAamp mini spin columns on QIAvac 24 or QIAvace 24 Plus: 24 VacValves. Should be orded in conjunction with VacConnectors (500) - Catalog No. 19407.  CNY                              【人血管生成素4(ANG-4)ELISA试剂盒现货】

19201       Collection Tubes (2-ml)     1000 Collection Tubes (2 ml)    CNY                             

19112       Buffer MTL (54 ml)            CNY                             

19046       Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps CNY                             

16191       QIAwell 96 Ultra Plasmid Kit (4)       For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, ａnd QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps     CNY                             

12991       QIAGEN Plasmid Plus Giga Kit (5)    QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12863       CompactPrep Plasmid Maxi Kit (25)         "For ultrafast purification of up to 200 µg or 750 µg Molecular Biology Grade plasmid or cosmid DNA for standard applications

CompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbench"       CNY                             

133281     NeXtalStock TRICINE pH 8.5 (200)            CNY                             

133275     NeXtalStock Spermidine (200)          CNY                             

337611     qBiomarker SYBR Green Mastermix (8)           CNY                             

4501303  artus EBV QS-RGQ Kit (24) RUO                CNY                             

4500363  artus HSV-1/2 QS-RGQ Kit (24) CE              CNY                             

949006     Autopure Cell Lysis Solution (3800 ml)               CNY                             

949002     Autopure Glycogen Solution (5 ml)           CNY                             

336313     SureSilencing shRNA Plasmid, Neomycin                   CNY                             

990382     Sample Tubes CB (2ml)              CNY                             

937255     QIAsymphony DSP DNA Midi Kit               CNY                              【人血管生成素4(ANG-4)ELISA试剂盒现货】

9010815  Cable, card-X to bar-code camera BL180                  CNY                             

330619     RT² SYBR Green Fluor FAST Mastermix 25ml            CNY                             

133245     NeXtalStock Sodium malonate pH 7.0 (200)             CNY                             

929703     QX 0.2 ml 12-Tube Strip (80)             CNY                             

330512     RT² SYBR Green Fluor qPCR Mastermix (12)             CNY                             

330141     Custom RT² PreAMP Primer Mix               CNY                             

9010603  Anschlagleiste fuer Samtrak P7xxx (9194)                 CNY                             

QT101-6   TUREscript One Step RT-PCR Kit（一步法反转录试剂盒）        2050                                               【人血管生成素4(ANG-4)ELISA试剂盒现货】

QT101-8   TUREscript SY Green qRT-PCR Kit （两步法荧光定量反转录试剂盒）      2050                                              

QD101-1  DNA MarkerⅠ（条带100.200.300.400.500.600）      "50T

"                                            

QD101-3  DNA Marker Ⅱ（条带100.300.500.700.900.1200） "50T

"                                            

QD101-5  DNA Marker Ⅲ（条带300.500.800.1500.2000.3000.5000）     "50T

"                                            

QD101-7  DNA Marker Ⅳ（条带500.1000.1500.3000.5000.8000） "50T

"                                            

QD101-9  DNA Marker Ⅴ（条带200.400.700.1000.1500.2000）      "50T

"                                            

QD101-11         DNA MarkerⅥ(条带250.1000.2500.5000.7000.10000)        "50T

"                                           

QD101-13         DNA Marker Ⅶ（条带300.500.1000.1500.2000.2500）    "50T

"                                            

QD101-17         50bp DNA Marker（条带50.100.150.200.250.300.250.400）     "50T

"                                            

QD101-19         50bp Plus（条带50.100.150.200.250.300.350.400.500.600.1000）   "50T

"                                            

QD101-21         100bp DNA Marker（100.200.300.400.500.600.700.800.900.1000.1500）         "50T

"                                            

QD101-23         100bp Plus（100.200.300.400.500.600.700.800.900.1000.1500.2000.3000.5000）         "50T

"                                            

QD101-25         200bp DNA Ladder  "50T

"                                            

QD101-27         250bp DNA Ladder（条带250.500.750.1000.1500.2000.2500.3000.5000）       "50T

"                                            

QD101-29         300bp DNA Ladder（条带300.600.900.1200.1500.1800.2500.3000.5000）       "50T

"                                            

QD101-31         500bp DNA Ladder（500.1000.1500.2000.2500.3000.3500.5000）   "50T

"                                             【人血管生成素4(ANG-4)ELISA试剂盒现货】

QD101-33         1kb  DNA Marker（条带1000.2000.3000.4000.5000.6000.8000.10000）        "50T

"                                            

QD101-35         1kb Plus（条带300.500.800.1000.1500.2000.3000.4000.5000.6000.8000.10000）  "50T

"                                            

QD101-43         DL2000（条带100.250.500.750.1000.2000）      "50T

"                                            

QD101-45         DL2000  Plus                                    （条带100.250.500.750.1000.2000.3000.5000） "50T

"                                            

QH406-1  λDNA/HindⅢ         "50T

"                                            

QH406-3  pUC19 DNA/MspⅠ "50T

"                                            

QC101-01          DH5α感受态细胞 40×100ul                                             

QC102-01          Top10感受态细胞  40×100ul                                             

QC103-01          JM109感受态细胞 20×100ul                                             

QC104-01          BL21(DE3)感受态细胞    20×100μl                                          

QC105-01          BL21(DE3) Competent cell       20×100ul                                      

QC121-01          高效感受态细胞制备试剂盒         100×100μl                                        

QC201-1   pSURE -T 载体连接试剂盒    20次                                             

 QC201-3          pSURE-T Simple载体连接试剂盒  20次(带T4 ligase）