人热休克因子1(HSF-1)ELISA试剂盒现货

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人热休克因子1(HSF-1)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人热休克因子1(HSF-1)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人热休克因子1(HSF-1)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人热休克因子1(HSF-1)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

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133181     NeXtalStock Citric acid pH 3.2 (200)                  CNY                             

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9018460  Pilot valve, QX           CNY                             

9015465  Interlock switch 1, for door               CNY                             

204952     QuantiFast Multiplex RT-PCR +R Kit (80)          CNY                             

133175     NeXtalStock Citric acid pH 2.0 (200)                  CNY                             

291023     mericon GMO Screen 35S-pat  (24)                 CNY                             

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380615     Investigator Hexaplex ESS Kit (100)                   CNY                             

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130917     NeXtal DWBlock ComPAS Suite                  CNY                             

QC024      包涵体溶解液         50ml                                              

QC025      蛋白复性液     100ml                                           

QC026      蛋白标准(5mg/ml BSA)  1ml                                        【人热休克因子1(HSF-1)ELISA试剂盒现货】

QC027      PMSF(100mM)          1ml                                       

QC028      5×Tris-甘氨酸电泳缓冲液    250ml                                           

QC029      SDS-PAGE电泳液(10X)    100ml                                           

QC030      SDS-PAGE蛋白上样缓冲液(5X)      2ml                                       

QC031      非变性PAGE蛋白上样缓冲液(5X)         2ml                                       

QC032      10%过硫酸铵 1.5ml                                            

QC033      30% 丙烯酰胺 (29:1)    100ml                                           

QC034      40% 丙烯酰胺 (39:1)    100ml                                           

QC035      0.5M DTT         2ml                                       

QC036      10% SDS   50ml                                              

QC037      TEMED     5ml                                       

QC038      1M Tris-HCl,pH6.8   50ml                                              

QC039      1.5M Tris-HCl,pH8.8         100ml                                           

QC040      Western转膜缓冲液 （20x）       100ml                                           

QC041      Western一/二抗稀释液          100ml                                           

QC042      Western封闭液(BSA型）      100ml                                            【人热休克因子1(HSF-1)ELISA试剂盒现货】

QC043      Western洗涤液       250ml                                           

QC044      Western一抗二抗去除液       250ml                                           

QC045      BCIP工作液 50mg/ml    100ul                                             

QC046      NBT工作液 50mg/ml    100ul                                             

QC048      10% Sodium Azide (叠氮钠)    100ml                                           

QC049      Protease Inhibitor  A(广谱性)        1ml                                       

QC050      Protease Inhibitor  B(适用于细菌)        1ml                                       

QC051      Protease Inhibitor  C(适用于哺乳动物)        1ml                                       

QC052      Protease Inhibitor  D(适用于真菌)       1ml                                       

QC053      Protease Inhibitor  E(适用于哺乳动物)        1ml                                       

QC054      Phosphatase Inhibitor M(广谱性)   1ml                                       

QC055      胰酶细胞消化液     100ml                                           

QC015      植物细胞质膜提取试剂盒     50T                                       

QC016      植物蛋白提取试剂盒     50T                                       

QC017      细菌总蛋白提取试剂盒         50T                                       

QC018      酵母总蛋白提取试剂盒         50T                                       

QC019      胞浆和核蛋白分步提取试剂盒     50T                                       

QC020      膜蛋白提取试剂盒         50T                                       

QC021      动物组织蛋白裂解液     50ml                                              

QC022      真核细胞蛋白裂解液     50ml                                              

QC023      RIPA裂解液   50ml