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您的位置:首页 > 产品展厅 >  化工原料 >  化学试剂 >  生化试剂 >  蜂蜜总抗检测试剂盒

蜂蜜总抗检测试剂盒

蜂蜜总抗检测试剂盒

  • 价 格: 电议
  • 型号:
  • 生 产 地:中国大陆
  • 访问:15次
  • 发布日期:2016/8/20(更新日期:2016/8/20)

齐一生物科技(上海)有限公司

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蜂蜜总抗检测试剂盒 货号RND 99043 Honey total antibiotic ELISA Test Kit 96T 美国REAGEN 齐一生物专业代理美国REAGEN,美国ABRaxis农残试剂盒,价格优惠货期短,部分产品现货供应
  • 详细内容
  • 公司简介
 齐一生物销售:0216034 8496181214 53965173021 04490

试剂盒名称:蜂蜜总抗检测试剂盒

格:96T

产品的用途:仅供科研究使用!

价格:电议,或咨询在线客服QQ,或者以邮件形式发到我司邮箱,齐一生物科技(上海)有限公司提供大量品牌进口原装,分装,以及稳定性强的国产ELISA试剂盒,性价比极高。欢迎您前来选购!

本试剂盒主要用于各种动物肌肉组织、肝脏、尿液、血清、饲料等中 残留定量检测!

 

蜂蜜总抗检测试剂盒试验原理:
该产品是固相夹心法酶联免疫吸附实验(ELISA.已知待测物质浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将待测物质和生物素标记的抗体同时温育。洗涤后,加入亲和素标记过的HRP。再经过温育和洗涤,去除未结合的酶结合物,然后加入底物AB,和酶结合物同时作用。产生颜色。颜色的深浅和样品中待测物质的浓度呈比例关系。

试剂盒内容及其配制

 

试剂盒自备材料
1
)蒸馏水。
2
)加样器:5ul10ul50ul100ul200ul500ul1000ul
3
)振荡器及磁力搅拌器等。

检测试剂盒安全性
1
)避免直接接触终止液和底物AB。一旦接触到这些液体,请尽快用水冲洗。
2
)实验中不要吃喝、抽烟或使用化妆品。
3
)不要用嘴吸取试剂盒里的任何成份

 

操作注意事项
1
)试剂应按标签说明书储存,使用前恢复到室温。稀稀过后的标准品应丢弃,不可保存。
2
)实验中不用的板条应立即放回包装袋中,密封保存,以免变质。
3
)不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质前使用。
4
)使用一次性的吸头以免交叉污染,吸取终止液和底物AB液时,避免使用带金属部分的加样器。
5
)使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6
)洗涤酶标板时应充分拍干,不要将吸水纸直接放入酶标反应孔中吸水。
7
)底物A应挥发,避免长时间打开盖子。底物B对光敏感,避免长时间暴露于光下。避免用手接触,有毒。实验完成后应立即读取OD值。
8
)加入试剂的顺序应一致,以保证所有反应板孔温育的时间一样。
9
)按照说明书中标明的时间、加液的量及顺序进行温育操作。

 

样品收集、处理及保存方法
1
)血清-----操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清和红细胞迅速小心地分离。
2
)血浆-----EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3
)细胞上清液---1000×g离心10分钟去除颗粒和聚合物。
4
)组织匀浆-----将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液
5
)保存------如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

蜂蜜总抗检测试剂盒

试剂盒试剂的准备
1
)标准品:标准品的系列稀释应在实验时准备,不能储存。稀释前将标准品振荡混匀。
2
)洗涤缓冲液(50×)的稀释:蒸馏水50倍稀释。

 

检测试剂盒操作步骤
1
)使用前,将所有试剂充分混匀。不要使液体产生大量的泡沫,以免加样时加入大量的气泡,产生加样上的误差。
2
)根据待测样品数量加上标准品的数量决定所需的板条数。每个标准品和空白孔建议做复孔。每个样品根据自己的数量来定,能使用复孔的尽量做复孔。标本用标本稀释液11稀释后加入50ul于反应孔内。
3
)加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内。立即加入50ul的生物素标记的抗体。盖上膜板,轻轻振荡混匀,37℃温育1小时。
4
)甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干。重复此操作3次。如果用洗板机洗涤,洗涤次数增加一次。
5
)每孔加入80ul的亲和链酶素-HRP,轻轻振荡混匀,37℃温育30分钟。
6
)甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干。重复此操作3次。如果用洗板机洗涤,洗涤次数增加一次。
7
)每孔加入底物AB50ul,轻轻振荡混匀,37℃温育10分钟。避免光照。
8
)取出酶标板,迅速加入50ul终止液,加入终止液后应立即测定结果。
9
)在450nm波长处测定各孔的OD值。

 

检测试剂盒局限
6
号标准品以上的结果为非线性的,根据此标准曲线无法得到精确的结果。

 

蜂蜜总抗检测试剂盒性能
1.
灵敏度:最小的检测浓度小于1号标准品。稀释度的线性。样品线性回归与预期浓度相关系数R值为0.990
2.
特异性:不与其它细胞因子反应。
3.
重复性:板内、板间变异系数均小于10%

检测试剂盒结果判断与分析
1
、仪器值:于波长450nm的酶标仪上读取各孔的OD
2
、以吸光度OD值为纵坐标(Y),相应的待测物质标准品浓度为横坐标(X),做得相应的曲线,样品的待测物质含量可根据其OD值由标准曲线换算出相应的浓度。

 

 齐一生物科技有限公司主要经营进口原装Elisa试剂盒,种类齐全,价格实惠,购买我公司产品的新老客户,可享免费代测实验服务,可检测人源,小鼠,大鼠,猪,狗,猴,兔,羊,鸡,牛,鸭子,鱼,植物等各种样本。全程提供技术支持。如有什么问题请给我们电话或者留言,告诉我您的需要,并留下您宝贵的意见和建议。

 

 

 

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9022121  Tool. Rotor Hub Height Jig, RGQ         CNY                           

9021404  RGQ MDx User manuals for IVD use(Europe)           CNY                           

9238610  Collet chuck          CNY                           

9238479  Motor, syringe, RapidPlate            CNY                           

9238152  Elution Microplate Adapter 8000, SBS          CNY                           

9237932  Syringe, 100µl              CNY                            【蜂蜜总抗检测试剂盒】

9240663  Nut, Flangeless,1/4-28, Black, AP        CNY                           

9240634  Power Supply, Main PCB, Aztec, AP196/98         CNY                           

9240630  Power Supply, 5VDC 1.5 AMP, AP96          CNY                           

9240593  Unload Valve, Pump, Pressure, AP196/98            CNY                           

9240562  Fuse, PCB, 315, Eppendorf, AP196/98        CNY                           

9240536  Motor Assy, SiNug, 17, All, AP96/196         CNY                           

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Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9240098  Re-Installation, BR EZ1  The Re-Installation, BR EZ1 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware and software check of instrument and provides user with operator refesher overview.  Re-installation includes labor and travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.     CNY                           

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Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

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9241211  Full Agreement Depot Repair, DML2000, 1y "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

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The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

 

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and complete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry and accessories are not included and should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

 

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.

 

Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring compliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

 

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QY-x1034       人鼻咽癌细胞HNE3                                       

QY-x1035       人鼻咽癌细胞HONE1                                    

QY-x1036       转基因鼻咽癌细胞系CNE1-lmp\l                                         

QY-x1037       低分化鼻咽癌细胞系SUNE-1                                       

QY-x1038       鼻咽癌细胞系SUNE-1亚株-5-8F                                         

QY-x1039       5-8转基因鼻咽癌细胞系F2-2                                       

QY-x1040       人鼻咽癌细胞系SUNE-1亚株6-10B                                    

QY-x1041       人鼻咽癌细胞系SUNE-1亚株13-9B                                    

QY-x1042       人鼻咽癌细胞系HNE2-LMP1/2                                     

QY-x1043       人鼻咽癌细胞系HNE2-LMP1                                       

QY-x1044       人鼻咽癌细胞系SUME-a                                       

QY-x1045       人淋巴细胞与仓鼠肺细胞杂交细胞FD4                                      

QY-x1046       人淋巴细胞与仓鼠肺细胞杂交细胞FD6                                      

QY-x1047       人小细胞肺癌细胞NCI-H446                                        【蜂蜜总抗检测试剂盒】

QY-x1048       人小细胞肺癌细胞NCI-H345                                        

QY-x1049       人小细胞肺癌细胞DMS153                                   

QY-x1050       人经典小细胞肺癌细胞NCI-H1688                                      

QY-x1051       人大细胞肺癌细胞NCI-H460                                        

QY-x1052       人大细胞肺癌细胞NCI-H661                                        

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科研之路,您我并肩同行!齐一生物具有完善的ELISA试剂盒开发平台,成熟的抗原、抗体研发系统,熟练掌握各种酶联技术,如双抗夹心法、(直接)竞争法、间接竞争法、阻断法、间接法、双抗原夹心法等方法,结合我公司的诊断试剂开发团队我们可以有效的将试剂盒开发为临床诊断级别,质量处于全国前茅。齐一生物针对中高端客户推出自主品牌QIYBO试剂盒为了保证客户用货质量,我公司采用纯进口原料现包被生产,质量稳定高.
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