齐帕特罗检测试剂盒

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供最优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【齐帕特罗检测试剂盒】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【齐帕特罗检测试剂盒】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【齐帕特罗检测试剂盒】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,最好做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【齐帕特罗检测试剂盒】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

133115     NeXtalStock Sodium sulfate (200)             CNY                             

965662     QIAamp Virus BioRobot 9604 Kit (12)     For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Carrier RNA, Caps       CNY                            

9240670  Reservoir, 1000ml, AP96/196            CNY                             

9240644  External BCR, Metrologic MS9521, AP             CNY                             

9240636  Power Entry Module, AP196/98                 CNY                             

9240616  Fuse, 5.0A, FNM, AP96             CNY                             

9240607  Fiber Optic Sensor Cable, 5M, AP98                  CNY                             

9240590  Purge Solenoid, Overflow Jar, AP96/196           CNY                             

9240573  Centrifuge RT-7, Sorvall, 115V, AP96                 CNY                             

9240551  Main PCB, Eppendorf, AP196/98                CNY                             

9240533  Motor Assy, SiNug, 23, CapU/D, AP96              CNY                             

9240524  Cable, Motor, Axis#1, Capper U\D, AP98                  CNY                             

9240347  Autopure, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9240243  Brushes, with spring (2), TR              CNY                             

9240097  Re-Installation, BR  The Re-Installation, BR facilitates quick implementation of the unit back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of  instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.   CNY                              【齐帕特罗检测试剂盒】

9239568  Needle, W-Drive, DISU, BR8, v2.0            CNY                             

9239561  Assy,Kit,Retrofit,Coherent-intoLX100               CNY                             

9019936  Pipetting head, 8 ch. , CAS3800/4800               CNY                             

9019491  PLATE 384 WELL ADAPTOR GENERIC                CNY                             

9238885  Cable, magnet lift, BS96           CNY                             

9238883  Keypad, BS96            CNY                             

9238865  #BR M96, Premium Agreement      "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9238859  #Full Agreement MDx, 2PM, 1yr     The Full Agreement MDx, 2 PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY                             

9241219  Warranty Performance Package, RCS               CNY                             

9241209  QIAsymphony SP, PM Agreement   An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for the maintenance visit. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.   CNY                             

9241212  Full Agreement Depot Repair, MSTVtx, 1yr    "The Full Agreement Depot Repair, MSTVtx provides full service coverage for the Multi-Specimen Tube Vortexer used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9241185  Sensor, interlock1, with cable, QX            CNY                             

9238823  Rect. carrier for 1 bottle 200 ml w/ cap           CNY                             

9238816  Rect. carrier for 12 Vacutainertubes 7ml                  CNY                             

9233298  Toothbelt, X-drive, P3xx0                   CNY                             

9237712  #BR M96, Installation               CNY                              【齐帕特罗检测试剂盒】

9233267  Motor, Y-drive, BR8000            CNY                             

9233211  Tubing, PVC transp., D5/d3mm, (per m)           CNY                             

NG0497   B19病毒抗体IgG\IgM检测试剂盒                血清         20T/40T                      

NG0498   轮状病毒抗原检测试剂盒              血清         20T/40T                      

NG0499   流行性乙脑病毒抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0500   柯萨奇病毒抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0501   麻疹病毒抗体IgG\IgM检测试剂盒               血清         20T/40T                      

NG0502   肾病综合症出血热病毒抗体IgG\IgM检测试剂盒               血清         20T/40T                       【齐帕特罗检测试剂盒】

NG0503   伤寒抗体检测试剂盒              血清         20T/40T                      

NG0504   副伤寒抗体检测试剂盒                   血清         20T/40T                      

NG0505   弓形虫抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0506   风疹病毒抗体IgG\IgM检测试剂盒               血清         20T/40T                      

NG0507   巨细胞病毒抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0508   单纯疱疹病毒Ⅰ抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0509   抗ENA抗体检测试剂盒                  血清         20T/40T                      

NG0510   抗核抗体ANA检测试剂盒             血清         20T/40T                      

NG0511   抗ds-DNA抗体检测试剂盒            血清         24T/48T                      

NG0512   抗SS-DNA抗体检测试剂盒            血清         20T/40T                      

NG0513   抗SM抗体检测试剂盒           血清         20T/40T                      

NG0514   抗组蛋白抗体检测试剂盒              血清         20T/40T                      

NG0515   类风湿因子IgA\IgM\IgG检测试剂盒           血清         20T/40T                      

NG0516   抗“O”试剂检测试剂盒                血清         20T/40T                      

NG0517   抗精子抗体IgA\IgM\IgG检测试剂盒           血清         24T/48T                      

NG0518   抗心磷脂抗体IgA\IgM\IgG检测试剂盒                血清         24T/48T                       【齐帕特罗检测试剂盒】

NG0519   抗子宫内膜抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0520   抗卵巢抗体IgA\IgM\IgG检测试剂盒           血清         24T/48T                      

NG0521   抗胰岛素自身抗体IAA检测试剂盒               血清         24T/48T                      

NG0522   抗滋养层细胞抗体IgG\IgM检测试剂盒               血清         24T/48T                      

NG0523   抗透明带抗体IgG\IgM检测试剂盒               血清         24T/48T                      

NG0524   抗HCG抗体IgG\IgM检测试剂盒                  血清         24T/48T                      

NG0525   早早孕试条检测试剂盒                   尿液         50T/100T                   

NG0526   排卵试条检测试剂盒              尿液         50T/100T                   

NG0527   血吸虫抗原检测试剂盒                   血清         20T/40T                      

NG0528   血吸虫抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0529   囊虫抗体IgG\IgM检测试剂盒               血清         20T/40T