蛋白酶KCAS:39450－01－6

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"蛋白酶KCAS:39450－01－6   英文名称:Proteinase K   规格：30u/mg   包装：100毫克      蛋白酶K(Sigma)

物理性状及指标：

外观：……………………冻干粉

溶解性：…………………水(1mg/mL),无色透明

简介：蛋白酶K是一个稳定、高活性丝氨酸蛋白酶。晶体和分子结构研究的表明其属于枯草杆菌蛋白酶家族，活性催化位点为 Asp39－His69－Ser224。在广泛的pH、缓冲盐，表面活性剂(SDS)和温度范围内保持稳定。在0.1－0.5% SDS中，蛋白酶K保留活性，降解各种蛋白质和核酸酶，在不影响DNA的完整性的前提下提取DNA。

用途及描述：科研试剂。蛋白酶K是DNA和RNA提取中使核酸酶失活。用于除去结合到如溶菌酶和核糖核酸酶A等阳离子蛋白上的内毒素；并用于确定酶在膜上的位置；用于石蜡嵌入组织部分的处理为抗体标记暴露抗原结合位点；

储存条件：−20°C"

QY-x1822     中国仓鼠卵细胞 成纤维细胞      CHO                                 

QY-x1823     中国仓鼠卵细胞GFP细胞    CHO-GFP                               

QY-x1824     人鼻咽癌细胞（低分化）     CNE-2                              

QY-x1825     SV40病毒转化的非洲绿猴肾成纤维细胞       Cos7                                 

QY-x1826     毛囊细胞     DPC                                  【蛋白酶KCAS:39450－01－6】

QY-x1827     人脐血内皮细胞       EVC304                                  

QY-x1828            EMPM2                            

QY-x1829     小鼠乳腺癌细胞       EMT-6                              

QY-x1830     人永生化表皮细胞   Hacat                                

QY-x1831     人早幼白血病细胞   HC-60                              

QY-x1832     人宫颈癌细胞    hela                            

QY-x1833            Hela-LC3B                              

QY-x1834     人 肝细胞癌 上皮样      Hep G2                             

QY-x1835     人 喉鳞状细胞癌     Hep-2                               

QY-x1836     人原髓细胞白血病细胞  HL-60                               

QY-x1837     人腹膜间皮细胞株   HMrSV5                                  

QY-x1838            HPME                              

QY-x1839     小鼠胚胎成纤维细胞      HSF1-/-                            

QY-x1840     小鼠胚胎成纤维细胞      HSF1+/+                                 

QY-x1841     小鼠胚胎成纤维细胞      HSF1-/+                                  

QY-x1842     人结肠癌     HT-29                                【蛋白酶KCAS:39450－01－6】

QY-x1843     人脐静脉内皮细胞   HUVE（18）                                 

QY-x1844     小鼠Lewis肺癌 LLC                                  

QY-x1845     人乳腺癌     MCF7                               

QY-x1846     小鼠神经成纤维细胞瘤  N2a       Albino mouse                           

QY-x1847     小鼠神经成纤维细胞瘤GFP N2a-GFP                                 

QY-x1848     人大肺细胞癌细胞   NCIH460                                

QY-x1849     NIH小鼠成纤维细胞      NIH 3T3                                  

QY-x1850     人卵巢癌细胞    OVCaR-3                                

QY-x1851     大鼠肾上腺髓质嗜铬细胞瘤 PC12                                

QY-x1852     猪卵母细胞 PFF                                  

QY-x1853     稳定表达BS的PFF PFF-BS                            

QY-x1854     耐药性Hep G2   R-Hep G2                                

9001905       #QIAgility (w/o PC) System      QIAgility (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.   CNY                          

800383  Open Reading Frame Search            CNY                          

9019052       Notebook 2 XP          CNY                          

9020200       ZLV PCB, USB, 18Q        CNY                          

9020580       Cable, DB9 M/F (UL) 6ft, DML 3000           CNY                          

9020588       Pulley, belt, DML 3000              CNY                          

9021016       Robot Stage, X Axis, VR, DCU              CNY                          

9013376       Maintenance Kit BR 8000 Research        CNY                          

9013279       BGR Upgrade-Kit Scanner PK80           CNY                          

9013272       Peristaltic Pump, RCS1             CNY                          

9013190       Motor, rotor, buffer track, BR8000          CNY                           【蛋白酶KCAS:39450－01－6】

132772  NeXtal Stock Jeffamine M-600 (200)      100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)    CNY                          

132620  NeXtal Stock Tricine (50)   1M Tricine - 50mL (4 x 12,5 ml tube)      CNY                          

132560  NeXtal Stock Magnesium chloride (50)   2M Magnesium chloride hexahydrate - 50mL (4 x 12,5 ml tube)  CNY                           

800171  Easy-Read Tubes, w/o prep       "Sequencing of plasmid DNA ａnd PCR products

DNA quality control not included

No repetition of sequencing

Data collected to maximal read length"     CNY                          

132546  NeXtal Stock Ferric chloride (50)     1M Ferric Chloride Hexahydrate - 50mL (4 x 12,5 ml tube) CNY                          

132008  EasyXtal 15-Well Tool X-Seal (20)  "20 empty 15-well crystallization plates with X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."     CNY                          

203645  HotStarTaq Plus Master Mix Kit (1000)  "For 1000 x 20 µl reactions: 12 x 0.85 ml HotStarTaq Plus Master Mix, containing 1000 units of HotStarTaq Plus DNA Polymerase total, 4 x 0.55 ml CoralLoad Concentrate, 8 x 1.9 ml RNase-Free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."   CNY                          

203603  HotStarTaq Plus DNA Polymerase (250) 1 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 CNY                          

203601  HotStarTaq Plus DNA Polymerase (50)   Non published.     CNY                          

201912  dNTP Set, PCR Grade 100mM, (4x100µl)     dNTP Set is a complete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 1000 x 50ul PCR reactions  CNY                          

201223  Taq PCR Core Kit (250)    "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."      CNY                          

180840  GeneRead qPCR SYBR Green/ROX MM (2)              CNY                          

180830  GeneRead qPCR SYBR Green/Fluor MM (2)              CNY                          

158767  Gentra Puregene Cell Kit (8 x 108)          CNY                          

34650    RGS·His Antibody, BSA-free (100 µg)    100 ug mouse anti-RGS(H)4 (lyophilized, BSA-free, for 1000 ml working solution)  CNY                          

32582    EasyXpress Disulfide Insect 10 rxns Kit          CNY                           【蛋白酶KCAS:39450－01－6】

32149    QIAexpress Type IV Kit    5 ug each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose. pQE-40 CNY                          

31014    Ni-NTA Spin Columns (50)       50 Ni-NTA Spin Columns, Collection Tubes   CNY                          

30600    Ni-NTA Fast Start Kit (6)         CNY                          

9021350       Piston, connecting rod, TLyser LT            CNY                          

9022027       Sensor, Y-axis, with cable, QXA              CNY                          

9002142       careHPV test Controller            CNY                          

286115  cador N. caninum PCR Reagent (96)              CNY                          

282603  virotype Influenza A RT-PCR Kit (24)             CNY