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您的位置:首页 > 产品展厅 >  化工原料 >  化学试剂 >  其他化学试剂 >  LipoJet™ (Ver. II) DNA In Vitro Transfection Reagen

LipoJet™ (Ver. II) DNA In Vitro Transfection ReagenSL100468

LipoJet™ (Ver. II) DNA In Vitro Transfection Reagen

  • 价 格: 2400元
  • 型号:SL100468
  • 生 产 地:中国大陆
  • 访问:27次
  • 发布日期:2016/10/11(更新日期:2023/10/7)

北京优尼康生物科技有限公司

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利用我们的创新和专有的脂质体共轭合成技术,LipoJet™转染试剂盒,运用了新型的含氟阳离子脂质体配方,同市场上的其他脂质体转染试剂有着显著 的区别,其特点是转染效率高、毒性小。LipoJet™转染试剂盒是最强大的基因 转导工具,适于几乎有所有体外基因转导的应用,包括质粒DNA、siRNA和shRNA转染和DNA/siRNA共转等。
  • 详细内容
  • 公司简介
 试剂盒内容:

- LipoJet™ 试剂,1.0ml,足够用1000DNA的转染(24孔板中0.5 ug DNA/)足够用1000siRNA转染(24孔板中10 nM siRNA/)
- LipoJet™转染缓冲液(5X ),按照最大化的转染效率配制,8.0ml浓缩液就可以制成40ml的工作液体。
产品特点:
适用于广泛的哺乳动物细胞
极高效率地转染DNAsiRNADNA/siRNA共转染
- 10 nM siRNA能达到95%的沉默效应
无血清和抗生素干扰
简单易用的操作步骤
适用于高通量的应用
- 细胞毒性最低
储存条件 
40C储存。若储藏合适,产品的稳定性能保持12个月以上。
LipoJet™体外转染试剂具有极广谱的转染活性。

细胞类型

DNA转染效率

siRNA转染效率

293, 293T
3T3, NIH 3T3
3T3-L1
A549
B16-F10
BNLCL.2
C2C12
C3H/10T1/2
Caco-2
Rat primary cardiomyocytes
CHO
COS1
COS7
Human bladder carcinomal cell
mES
iMEF
HBEC
HCT116
Hela
HepG2
HT-29
HuH-7
LNCaP
MC3T3-E1
MCF10A
MCF7
MDA-MB-231
MDCK
MEF
Human primary melanocytes
Human primary melanoma
Human immortalized microglial
mIMCD-3
MRC-5
N2A
PC3
RAW 264.7
RPE
SH-SY-5Y
SK-OV-3
U-2OS
VERO

85%
-
30%
80%
75%
55%
80%
60%
20%
10%
80%
70%
75%
80%
50%
55%
45%
75%
80%
60%
45%
45%
45%
50%
40%
50%
50%
20%
50%
50%
50%
50%
50%
50%
68%
70%
45%
30%
75%
70%
80%
40%

-
90% at 20 nM
-
90% at 20 nM
-
-
90% at 40 nM
-
80 % at 30 nM
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
85% at 30 nM
85% at 30 nM
-
-
-
-
-
-
-
-
90% at 30 nM
-
-
-


以下图例表明LipoJet™体外转染试剂盒出色的DNA和siRNA转染效率 。
LipoJet Transfection Reagent
A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel).  pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The transfection efficiency were analyzed via FACS 48 hours post transfection.
LipoJet_Cell_Viability
A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.  pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection. 
LipoJet_LIPO2000_POLYJET_Comparison_HEK293

A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.
LipoJet_LIPO2000_POLYJET_Comparison_HELA
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.



LipoJet™试剂介导的DNA/siRNA共转染表现出色的基因沉默效应。分别由LipoJet™试剂转染GFPcDNA (左图, 0.25µg/孔,24孔板)作为对照和共转染GFPcDNA (0.25µg/孔,24孔板)/GFP的siRNA (终浓度10 nM右图)HEK293细胞 (上图), Hela细胞(中图)和SaoS-2细胞(下图)转染24小时后,使用尼康Eclipse荧光显微镜检查GFP荧光。结果显示,GFPcDNAGFPsiRNA共转染导致GFP的表达被显著抑制。


LipoJet_HUVEC_mCherry-FITC-siRNA

Exceptional DNA/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) and FITC conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) and nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection.  The pictures were given from Dr. Pan Kong of USC as courtesy.  
LipoJet_EG5_HEK293
LipoJet_EG5_HELA
Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet
™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope. 





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