您的位置:首页 > 产品展厅 > 仪器仪表 > 专用仪器仪表 > 其他专用仪器仪表 > TaqMan® Fast Advanced Master Mix 4444963 4444965
TaqMan® Fast Advanced Master Mix has been designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes) with results equal to or better than what is achieved today. TaqMan® Fast Advanced Master Mix contains AmpliTaq® Fast DNA Polymerase, uracil-N glycosylase (UNG), dNTPs with dUTP, ROX™ dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.
Features of the TaqMan® Fast Advanced Master Mix include:
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
• Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
• Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamlessly transitions into your experiments—validated with Applied Biosystems® TaqMan® Assays for Gene Expression and microRNAs, and TaqMan® Array Micro Fluidic Cards
Best-in-Class Performance
TaqMan® Fast Advanced Master Mix has been designed to provide performance equal to or better than the results you currently expect from your standard master mix. The master mix has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. The impressive sensitivity of the mix showcased in Figure 1 compares the Ctvalues of TaqMan® Fast Advanced Master Mix and TaqMan® Universal PCR Master Mix across a panel of gene expression assays.
Benchtop Stability for High-Throughput Handling and Convenience
TaqMan® Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. Figure 2 shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.
For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running your plates upon assembly.
Optimized for Multiplexing
We realize that confidence is paramount when it comes to your results. For added confidence in the results you see in every well, TaqMan® Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). Figure 3 shows results for the experimental target β-actin (gene name ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.
Detection Method: | Primer-Probe Detection |
---|---|
For Use With (Equipment): | 7500 Fast System,7500 System,7900HT System,QuantStudio™ 12k Flex,QuantStudio™ 3,QuantStudio™ 5,QuantStudio™ 6,QuantStudio™ 7,StepOne™,StepOnePlus™,ViiA™ 7 System |
Reaction Speed: | Fast |
PCR Method: | qPCR |
Sample Type (General): | DNA |
Number of Reactions: | 1000 reactions |
Polymerase: | AmpliTaq® Fast DNA Polymerase |
Primer-Probe Compatibility: | TaqMan® |
Product Line: | TaqMan™ |
Product Size: | 2 x 5 mL |
Sample Type (Specific): | DNA (Genomic),cDNA |
1.德国Greiner培养板、培养皿、离心管、冻存管、培养瓶、接种环、接种针等;
2.美国Wheaton 匀浆器、组织研磨器、双侧壁细胞培养瓶、悬浮细胞培养瓶、细胞用搅拌器、安培瓶等玻璃仪器、培养箱,搅拌器等。
3.美国kimble 蓝盖瓶、匀浆器、离心管,巴氏吸管等玻璃仪器;
4.加拿大Labplas 的无菌采样袋、均值袋;
5.美国Nalgene 过滤装置、塑料广口瓶、放水桶、低温冰盒、低温标签、记号笔、离心管、冻存管、便携液氮罐、无菌培养基瓶、手动真空泵等。
6.美国ABI PCR板、PCR管、PCR盖子、八连排管、膜等。
7.德国Brand 移液器,电动移液器,瓶口分配等
8.德国艾本德eppendorf 移液器、分配器等
9.法国Hyphen Biomed 的出血、凝血类试剂 ;
10. 德国Chemvck 防腐隔膜泵、无油泵、真空泵、蠕动泵等
11. 瑞士Keller 小型快速玻璃珠灭菌器、玻璃珠等
12. 美国Millpore 细胞电阻仪、超滤管、膜、针头滤器等
13. 美国 BioSpec研磨珠均质器、手持匀浆机等
14. 自产镍洛合金接种环、接种针、核酸染料、通用水试剂培养基等。实验室 蓝盖瓶 218012458 218012955 218013651
具有显著 特性的 DURAN ® 实验室玻璃瓶己赢得市场好评多年。经过不断发展和提高,DURAN GROUP在原有产品的基础上推出了一系列设计精良的优质产品。为用户提供两种选 择,一种是单
PowerUp™ SYBR® Green Master Mix A25742 A25777
PowerUp™ SYBR® Green Master Mix A25742 A25777 A25778 A25918
① 凡本网注明"来源:易推广"的所有作品,版权均属于易推广,未经本网授权不得转载、摘编或利用其它方式使用。已获本网授权的作品,应在授权范围内
使用,并注明"来源:易推广"。违者本网将追究相关法律责任。② 本网凡注明"来源:xxx(非本网)"的作品,均转载自其它媒体,转载目的在于传递更多信息,并不代表本网赞同其观点和对其真实性负责,且不承担此 类作品侵权行为的直接责任及连带责任。如其他媒体、网站或个人从本网下载使用 ,必须保留本网注明的"稿件来源",并自负版权等法律责任。
③ 如涉及作品内容、版权等问题,请在作品发表之日起两周内与本网联系,否则视为放弃相关权利。
④易推广页面显示产品信息均由企业自主发布,信息内容真实性、准确性与合法性由相关企业负责,易推广对此不承担任何责任,如遇非法或侵权信息欢迎监督,请联系QQ:1273397930或者发邮件至:1273397930@qq.com,如有确实证件证明属实,本站将对其删除处理,谢谢!
⑤ 本信息由注册会员:北京优尼康生物科技有限公司发布并且负责版权等法律责任。
易推广客服微信