TaqPath™ ProAmp™ Master Mix A30866 A30867

描述

TaqPath™ ProAmp™ Master Mixes are versatile ｍaster mixes developed for high-throughput genotyping ａnd copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mixes are designed to deliver sensitive ａnd reproducible results from genomic DNA targets. The simple genotyping ａnd copy number variation workflow ａnd reproducible performance even in the presence of inhibitors provides confidence in results.

Two TaqPath ProAmp Master Mix formulations for different throughput needs

• TaqPath ProAmp Master Mix—includes ROX™ passive reference dye for lower multiplexing of up to 3 targets
• TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE™ passive reference dye for higher multiplexing of up to 4 targets

Benefits of TaqPath ProAmp Master Mixes

•Exceptional data quality—high specificity, dynamic range*, ａnd reproducibility for genotyping ａnd copy number determination even in the presence of inhibitors
•Tolerance of inhibitorscommon in samples prepared from human or animal sources (buccal swabs, blood, ａnd card punches)
•72-hour pre-PCR benchtop stabilityfor automated workflows, enabled by our proprietary Dual-Lock™ Taq DNA hot-start mechanism
•Multiplexing breadth—two formulations that enable single to four-target detection per reaction
•Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mixes have been optimized to yield accurate ａnd reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. Figure 1 shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan Genotyping Assays using crude lysate from blood samples. TaqPath ProAmp Master Mixes have been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.