您的位置:首页 > 产品展厅 > 化工原料 > 化学试剂 > 生化试剂 > MEGAscript™ T7 Transcription Kit现货促销价格
品牌: | Thermo-life |
货号: | AM1333 |
供应商: | 上海徕创生物科技有限公司 |
数量: | 充足 |
保质期: | 三年 |
保存条件: | 低温 |
规格: | EACH
|
The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.
Using the MEGAscript® T7 Kit
The MEGAscript® T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 µg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.
Applications
The MEGAscript® T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. The MEGAscript® T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.
Accessory products
MessageAmp™ aRNA amplification kits are powered by MEGAscript® technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitrotranscription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).
Starting Material: | Linearized DNA, PCR Products |
Promoter: | T7 |
RNA Yield: | 100 µg |
Final Product: | In Vitro Transcribed RNA |
Product Line: | Ambion™, MEGAscript® |
Product Size: | 25 reactions |
Shipping Condition: | Dry Ice |
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
CCK试剂盒细胞增殖及毒性检测-Cell Counting Kit
CCK试剂盒细胞增殖及毒性检测-Cell Counting Kit CCK比XTT和MTS更加稳定,使实验结果更加稳定 CCK和MTT、XTT等相比线性范围更宽,灵敏度更高
CellTiter-Glo®发光法细胞活力检测试剂盒 是通过对ATP 进行定量测定来检测培养物中活细胞数目的一种均质检测方法,ATP 是活细胞新陈代谢的一个指标。
微探TM支原体检测试剂盒报价 支原体在分类学上是处于细菌和病毒之间的一种微生物,是细胞培养中最常见的一种污染源,被支原体感染的细胞培养物其正常的生长和代谢会受到极大的影响。而重组表达的蛋白质,其活性也
① 凡本网注明"来源:易推广"的所有作品,版权均属于易推广,未经本网授权不得转载、摘编或利用其它方式使用。已获本网授权的作品,应在授权范围内
使用,并注明"来源:易推广"。违者本网将追究相关法律责任。② 本网凡注明"来源:xxx(非本网)"的作品,均转载自其它媒体,转载目的在于传递更多信息,并不代表本网赞同其观点和对其真实性负责,且不承担此 类作品侵权行为的直接责任及连带责任。如其他媒体、网站或个人从本网下载使用 ,必须保留本网注明的"稿件来源",并自负版权等法律责任。
③ 如涉及作品内容、版权等问题,请在作品发表之日起两周内与本网联系,否则视为放弃相关权利。
④易推广页面显示产品信息均由企业自主发布,信息内容真实性、准确性与合法性由相关企业负责,易推广对此不承担任何责任,如遇非法或侵权信息欢迎监督,请联系QQ:1273397930或者发邮件至:1273397930@qq.com,如有确实证件证明属实,本站将对其删除处理,谢谢!
⑤ 本信息由注册会员:上海徕创生物科技有限公司发布并且负责版权等法律责任。
易推广客服微信