SouthernBiotech 小鼠抗鸡IgM二抗应用说明书

艾美捷/小鼠抗鸡IgM二抗应用：

FC – Quality tested

IHC-FS – Reported in literature

ICC – Reported in literature

IP – Reported in literature

Block – Reported in literature

艾美捷/小鼠抗鸡IgM二抗储存：

1.纯化（UNLB）抗体以0.5 mg纯化免疫球蛋白的形式提供于1.0 mL硼酸盐缓冲盐水中，pH值为8.2。不添加防腐剂或含胺缓冲盐。在2-8°C下储存。

2.荧光素（FITC）结合物以0.5 mg/1.0 mL PBS/NaN3的形式提供。在2-8°C下储存。

3.在1.0毫升PBS/NaN3中以0.5毫克的形式提供生物素（BIOT）结合物。在2-8°C下储存。

4.R-藻红蛋白（PE）结合物以0.1 mg/1.0 mL PBS/NaN3和稳定剂的形式提供。在2-8°C的温度下储存。不要冻结

5.保护荧光色素结合物不受光照。如果按照指示储存，试剂在标签上显示的时间内是稳定的。

小鼠抗鸡IgM二抗参考文献：

1. Ewert DL, Munchus MS, Chen CH, Cooper MD. Analysis of structural properties ａnd cellular distribution of avian Ia antigen by using monoclonal

antibody to monomorphic determinants. J Immunol. 1984;132:2524-30. (Immunogen, FC, ICC, IP, Block, Quail, Dove, Pigeon, & Duck Reactivity)

2. Wang X, Efr GF. Apoptosis in feathers of Smyth line chickens with autoimmune vitiligo. J Autoimmun. 2004;22:21-30. (IHC-FS)

3. O＇Halloran EK, Oesterle EC. Characterization of leukocyte subtypes in chicken inner ear sensory epithelia. J Comp Neurol. 2004;475:340-60. (IHCFS)

4. Das SC, Nagasaka N, Yoshimura Y. Changes in the localization of antigen presenting cells ａnd T cells in the utero-vaginal junction after repeated

artificial insemination in laying hens. J Reprod Dev. 2005;51:683-7. (IHC-FS)

5. Subedi K, Yoshimura Y. Expression of MHC class I ａnd II in growing ovarian follicles of young ａnd old laying hens, Gallus domesticus. J Poult Sci.

2005;42:101-9. (IHC-FS)

6. Yoshimura Y, Fukui T, Nishibori M, Isobe N. Effects of age ａnd gonadal steroids on the localization of antigen presenting cells in the epididymis of the

male chicken, Gallus domesticus. J Reprod Dev. 2006;52:363-71. (IHC-FS)

7. Bakri Y, Sarrazin S, Mayer UP, Tillmanns S, Nerlov C, Boned A, et al. Balance of MafB ａnd PU.1 specifies alternative macrophage or dendritic cell

fate. Blood. 2005;105:2707-16. (FC, ICC)

8. Niikura M, Kim T, Hunt HD, Burnside J, Morgan RW, Dodgson JB, et al. Marek＇s disease virus up-regulates major histocompatibility complex class II

cell surface expression in infected cells. Virology. 2007;359:212-9. (ICC)

9. Rautenschlein S, Subramanian A, Sharma JM. Bioactivities of a tumour necrosis-like factor released by chicken macrophages. Dev Comp Immunol.

1999;23:629-40. (FC)

10. Chrząstek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T ａnd B cells in chicken. Pol J Vet Sci.

2013;16:551-4. (FC)

来源：https://www.amyjet.com/products/8300-01.shtml