人巨噬细胞移动抑制因子试剂盒

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产品名称：上海人巨噬细胞移动抑制因子试剂盒,人巨噬细胞移动抑制因子试剂盒
联系电话：021—60449724，13296089724
操作步骤：
参照说明书
灵敏度：
具体请查看说明书.
检测范围（RANGN）：
具体请查看说明书.
参考值：
产品用途:
科研ELISA检测试剂盒.提供实验代测服务.您只需将需要检测的种属(Human,Rat,Mouse,Rabbit,Monkey, Pig……)和检测指标(介素类、因子类)及标本数量(48T/96T)通知公司业务员即可。在接到客户标本当日起，现货产品周内将检测报告交到客户手中！
产品范围:
人,小鼠,大鼠,猪,兔,其它动物细胞因子;细胞凋亡,活性多肽、自身抗体 ,血栓与止血,
骨代谢,肝纤维化,肿瘤,激素内分泌、自身抗体科研ELISA检测试剂盒

试剂的准备：

1.   标准品：标准品的系列稀释应在实验时准备，不能储存。稀释将标准品振荡混匀。
2.   洗涤缓冲液（50×）的稀释：蒸馏水50倍稀释。

试剂盒性能：

 1.   灵敏度：小的检测浓度小于1号标准品。稀释度的线性。样品线性回归与预期浓度相关系数R值为0.990。

2.   特异性：不与其它细胞因子反应。

3.   重复性：板内、板间变异系数均小于10%。

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer ａnd each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Storage ａnd validity
1．Storage：  2-8℃.
2．validity： six months.