中英文说明书丨艾美捷MYC（人）重组蛋白介绍

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis ａnd cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement ａnd translocation of this gene have been associated with a variety of hemaoietic tumors, leukemias ａnd lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) ａnd a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt＇s lymphomas, suggesting its importance in the normal function of this gene.

Product Description:Human MYC full-length ORF ( ENSP00000259523, 1 a.a. - 439 a.a.) recombinant protein with GST-tag at N-terminal.

Host:Wheat Germ (in vitro)

Theoretical MW (kDa):75.2

Interspecies Antigen Sequence:Mouse (91); Rat (90)

Preparation Method:in vitro wheat germ expression system

Purification:Glutathione Sepharose 4 Fast Flow

Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Storage Instruction:Store at -80°C. Aliquot to avoid repeated freezing ａnd thawing.

Note:Best use within three months from the date of receipt of this protein.

Applications：

Enzyme-linked Immunoabsorbent Assay

Western Blot (Recombinant protein)

Antibody Production

Protein Array

艾美捷MYC（人）重组蛋白中文说明：

宿主：小麦胚芽（体外）。

理论分子量（kDa）：75.2

种间抗原序列：小鼠（91）；大鼠（90）。

制备方法:体外小麦胚芽表达系统

纯化方法:谷胱甘肽对苯二甲酸脂4号快速流。

储存缓冲液：50 mM Tris-HCI，10 mM 还原谷胱甘肽，洗脱缓冲液的pH=8.0。

储存指导：储存在-80℃。等分，以避免反复冷冻和解冻。

艾美捷MYC（人）重组蛋白应用：

酶联免疫吸附试验

WB印迹（重组蛋白）

抗体生产

蛋白阵列

艾美捷MYC（人）重组蛋白相关研究：

CG0011MYC Split CISH Probe

CG0021MYC CISH Probe

DP0144MYC(phospho T358) & MYC Protein Phosphorylation Antibody Pai

rFG0009c-myc/CEN8p FISH Probe

FG0065c-myc/CEN8p FISH Probe

FG0099MYC (Orange) FISH Probe

FG0117MYC/CEN8q FISH Probe

FP0015mutaFISH™ c-Mycwt RNA Probes

FS0006MYC Split FISH Probe

FT0026IGH/MYC DY Translocation FISH Probe

FT0036IGH/MYC Translocation FISH Probe

H00004609-D01MYC MaxPab rabbit polyclonal antibody (D01)

H00004609-KMYC rabbit monoclonal antibody

H00004609-M02MYC monoclonal antibody (M02), clone 1G7

H00004609-PW1MYC (Human) IP-WB Antibody Pair

H00004609-Q01MYC (Human) Recombinant Protein (Q01)

H00004609-W01PMYC DNAxPab

来源：https://www.amyjet.com/products/H00004609-P01.shtml