中英文说明书丨艾美捷T7 RNA聚合酶介绍

Activity: 1 μl T7 RNA Polymerase HC generates >/= 30 μg RNA in 10 min at 37°. This is comparable to 1000 U of NEB’s T7 RNA Polymerase (High Concentration), #M0460T (activity assay conditions: 1x T7 IVT Set-up Buffer supplemented with 10 mM DTT, 12 mM MgCl2, 3 mM NTPs, 1 μl T7 RNA Polymerase HC ａnd 1 μg DNA template (1.4 kpb RNA transcript) in 20 μl. RNA yield was determined with a fluorescence microplate assay.)

Cat. No.：RNT-008

Amount：50 μl (2 μg/μl)

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

avoid freeze/thaw cycles

Shelf Life: 12 months

Purity: ≥ 95 % (SDS-PAGE)

Form: liquid

Concentration: 2.0 – 2.2 μg/μl (A280, ɛ = 140 L mmol-1 cm-1 [1] in T7 RNA Polymerase HC Storage Buffer)

Description:

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter.

The highly concentrated version T7 RNA Polymerase HC is ideally suited for set-up ａnd optimization of high yield in vitro transcription reactions. The provided T7 IVT Set-up Buffer, MgCl2 ａnd DTT solutions enable testing of different reaction conditions such as nucleotide/ MgCl2 concentrations.

Recommendation for assay set-up with minimal optimization: HighYield T7 RNA Synthesis Kit (#RNT-101).

艾美捷T7 RNA聚合酶中文说明：

1μl T7 RNA聚合酶HC在37°下10分钟内产生>/=30μg RNA。这相当于1000 U NEB的T7 RNA聚合酶（高浓度），#M0460T（活性测定条件：1x T7 IVT建立缓冲液，补充10 mM DTT、12 mM MgCl2、3 mM NTPs、1μl T7 RNA聚合酶HC和1μg DNA模板（1.4 kpb RNA转录物），20μl。用荧光微板测定法测定RNA产量。）

艾美捷T7 RNA聚合酶内容：

T7 RNA聚合酶HC

1x 50μl（2μg/μl）

50 mM Tris-HCl、1 mM EDTA、100 mM NaCl、5 mM DTT、0.1%Triton-X-100、50%甘油（v/v），pH 8.0

T7 IVT设置缓冲区

1x 200μl（10倍）

400 mM Tris-HCl（pH 8.0），20 mM精蛋白

DTT

1x 100μl（100 mM）

氯化镁

1x 200μl（200 mM）

相关研究：

RNT-101，高产T7 RNA合成试剂盒

来源：https://www.amyjet.com/brand/Enzo-Life-Sciences.shtml