中英文说明书丨艾美捷双链RNA定量试剂盒

Product Name

Double-stranded RNA (dsRNA), ELISA Kit

Full Product Name

Double-stranded RNA (dsRNA) ELISA kit (J2 based)

Product Synonym Names

dsRNA ELISA kit

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

Clonality

Monoclonal

Isotype

Mouse IgG2a kappa/IgM kappa

Clone Number

J2/K2

Species Reactivity

General

Source

The two dsRNA antibodies used in this kit were produced as follows: Female DBA/2 mice were injected intraperitonially with a mixture of 50ug L-dsRNA ａnd 75ug methylated bovine serum albumin, emulsified in complete Freund＇s adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clones J2 ａnd K2.

Product

Double-stranded RNA (dsRNA) ELISA kit containing the following reagents for 200 tests (2x96 wells):

Reagent 01: 1 vial of coating antibody (store at -20 degree C

Reagent A: 1 vial of 142 bp dsRNA as positive control (store at -20 degree C)

Reagent B: 1 vial of dsRNA-specific detecting antibody (in RPMI + 5% FBS, store at +4 degree C or, preferably, at -20 degree C)

Reagent C: 1 vial of HRP-conjugated F(ab＇)2 Fragment of goat-anti mouse secondary antibody (store at +4 degree C or at -20 degree C)

Reagent D: 1 vial of TMB substrate solution (store at +4 degree C, keep in dark

艾美捷双链RNA定量试剂盒中文说明：

艾美捷双链RNA定量试剂盒，利用双抗夹心ELISA原理，检测核酸提取物中的病毒dsRNA或非病毒来源的天然或合成的dsRNA，以及检测人工合成(m)RNA制剂中的dsRNA杂质。

艾美捷双链RNA定量试剂盒基本参数：

品名：双链RNA（dsRNA），ELISA试剂盒

同义词名称：dsRNA ELISA试剂盒

仅供研究使用。不适用于诊断程序。

克隆性：单克隆

同位素：小鼠IgG2a-kappa/IgM-kappa

克隆编号：J2/K2

物种反应性：全体的

相关研究：

MBS574013   Mouse anti double-stranded RNA (J2)   0.2mg

MBS574015   Mouse anti double-stranded RNA (K1)   0.2mg

MBS574017   Mouse anti double-stranded RNA (K2)   5ml

MBS574019   Mouse anti double-stranded RNA (J5)   0.2mg

MBS574021   Mouse anti double-stranded RNA (J2, J5 ａnd K1) Comparison Kit   3x0.1mg

来源：https://www.amyjet.com/brand/double-stranded-dsrna.shtml