中英文说明书丨艾美捷内毒素检测试剂盒

Background: The EndoAlert™ Endotoxin Plate Kit is a kinetic, colorimetric assay for the quantitative determination of bacterial Endotoxin in aqueous solutions. Endotoxin, a bacterial lipopolysaccharide, is one of the major cell wall components of most gram-negative bacteria. The EndoAlert™ Endotoxin Plate Kit detects low levels of Endotoxin ａnd is therefore a useful tool to assess the integrity of biological ａnd environmental samples. The detection ranges from 0.01 - 10 Endotoxin units (EU/mL). The EndoAlert™ Endotoxin Plate Kit is a quantitative version of the reaction first described by Levin ａnd Bang in 1968. The test is based upon an enzymatic cascade where Endotoxin activates Factor C in Limulus Amebocyte Lysate (LAL) which in turn activates Factor B. Factor B activates Proclotting Enzyme which then activates Clotting Enzyme. A colorless synthetic peptide substrate is hydrolyzed by Clotting Enzyme to generate a yellow color which can be measured by a spectrophotometer at 405 nm. The degree of color resulting from the reaction is proportional to the amount of Endotoxin in the test sample ａnd can be calculated using a standard curve.

Label: Unconjugated

Expiration: See kit ｉnsert for complete instructions

Storage Condition:Store all kit reagents at 2-8 °C in the dark. Avoid storing kits for extended periods (more than 24 hours) at room temperature. See kit protocol for complete details.

Synonyms: Endotoxin Test Kit, Endotoxin level, Endotoxin Detection, bacterial Endotoxin, Limulus Amebocyte Lysate (LAL)

Assay Dilutions: All assays should be optimized by the user. Recommended dilutions (if any) may be listed below

艾美捷内毒素检测试剂盒中文说明：

艾美捷内毒素检测试剂盒是一种用于定量测定水溶液中细菌内毒素的动力学比色测定法。内毒素是一种细菌脂多糖，是大多数革兰氏阴性细菌的主要细胞壁成分之一。该检测低水平的内毒素，因此是评估生物和环境样品完整性的有用工具。检测范围为0.01-10内毒素单位（EU/mL）。

艾美捷内毒素检测试剂盒基本参数：

中文名称：EndoAlert™ 内毒素检测试剂盒

英文名字：EndoAlert™ Endotoxin Plate Kit - KMA-0100

编号：KMA-0100

规格：1Kit

检测范围：0.01-10内毒素单位（EU/mL）。孵育60分钟。

将所有试剂盒试剂储存在2-8°C的黑暗中。避免在室温下长时间（超过24小时）存放试剂盒。

艾美捷内毒素检测试剂盒原理：

该是Levin和Bang于1968年shou次描述的反应的定量版本。该测试基于酶联反应，其中内毒素激活鲎血细胞裂解物（LAL）中的因子C，进而激活因子B。因子B激活Proclotting酶，然后激活凝血酶。无色合成肽底物通过凝集酶水解以产生黄色，该黄色可通过分光光度计在405nm下测量。反应产生的颜色程度与测试样品中内毒素的量成比例，并且可以使用标准曲线计算。

来源：https://www.amyjet.com/products/KMA-0100.shtml