ImmunoChemistry丨ImmunoChemistry艾美捷MitoPT JC-1测定方案

线粒体膜电位的丧失表明细胞凋亡。ICT的MitoPT JC-1测定利用电位荧光染料JC-1检测线粒体膜通透性和膜去化。JC-1染料具有分散在整个分子结构中的离域正电荷。此外，其亲脂性溶解性使其易于渗透膜并穿透活细胞。JC-1染料进入带负电荷的线粒体，在那里聚集并在激发时发出橙色荧光。当凋亡细胞中线粒体膜电位崩溃时，MitoPT JC-1分布在整个细胞溶胶中。使用流式细胞仪、荧光平板读取器或荧光显微镜分析结果。

ImmunoChemistry艾美捷MitoPT JC-1检测试剂盒化学性质：

试剂名称JC-1

靶向线粒体去化

激发/发射488 nm/590 nm和527 nm

分析方法流式细胞术、荧光显微镜、荧光板读取器

样品类型细胞培养

储存-20°C

试剂盒组分：

Kit 911: 400 Tests

MitoPT JC-1 Reagent, 100 Tests, #6260

10X Assay Buffer, 125 mL, #6259

CCCP, 50 mM, 600 µL, #6258

Kit Manual

Kit 924: 100 Tests

MitoPT JC-1 Reagent, 100 Tests, #6261

10X Assay Buffer, 60 mL, #685

CCCP, 50 mM, 125 µL, #6257

Kit Manual

ImmunoChemistry艾美捷MitoPT JC-1检测试剂盒相关文献：

Funakoshi, A;Honda, T;Ito, T;Tokura, Y. Cholecystokinin receptor antagonist suppresses melanoma growth by inducing apoptosis of tumor cells. JID Innovations. 2022 September 1; doi: 10.1016/j.xjidi.2022.100153. Abstract

Yoshioka, H;Komura, S;Kuramitsu, N;Goto, A;Hasegawa, T;Amizuka, N;Ishimoto, T;Ozasa, R;Nakano, T;Imai, Y;Akiyama, H. Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures. Journal of bone ａnd mineral metabolism. 2022 August 10; doi: 10.1007/s00774-022-01354-2. Publication

Zhong, G;Long, H;Zhou, T;Liu, Y;Zhao, J;Han, J;Yang, X;Yu, Y;Chen, F;Shi, S. Blood-brain barrier Permeable nanoparticles for Alzheimer＇s disease treatment by selective mihagy of microglia. Biomaterials. 2022 August 8; doi: 10.1016/j.biomaterials.2022.121690. Full Text

ImmunoChemistry Technologies简称"ICT",提供各种细胞蛋白酶、细胞凋亡与细胞毒性检测试剂盒等研究工具，同时包含高质量高灵敏度的 ELISA开发试剂。艾美捷科技是ImmunoChemistry的中国代理商，为科研工作者提供优质的产品与服务。

来源：https://www.amyjet.com/brand/Immunochemistry.shtml