内皮细胞生长添加剂（ECGF/ECGS）丨艾美捷解决方案

艾美捷Relia Tech内皮细胞生长添加剂也可用于培养没有饲养细胞的杂交瘤细胞（Pintus，1983）。这些脑提取物中的活性因子已被鉴定为aFGF的变体（Burgesse，1985）。该ECGF产品补充了生理浓度的人rec.VEGF-A，以支持培养中血管内皮细胞的生长和分化。

艾美捷Relia Tech内皮细胞生长添加剂相关参数说明：

Cat-Nr. 300-090-B

Size 6 mg

Source Cattle

Formulation lyophilized (freeze-dried)

Purification Crude extract

Biological Activity Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 µg/ml, optimal concentration with heparin (30 µg/ml) is about 12 µg/ml.

Species Reactivity human cells, mouse cells, rat cells, cattle cells, pig cells

Buffer water

Reconstitution Reconstitute the contents of the vial in 2 ml of prewarmed (37°C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.

含VEGF 165用于微血管内皮细胞培养：

ECGFpro1  300-090-B 6mg

ECGFpro1 +Heparin(肝素) 300-090H-B 6mg

含VEGF-C用于淋巴内皮细胞培养：

ECGFpro2  300-090-L 6mg

ECGFpro2 +Heparin/肝素 300-090H-L 6mg

ECGF用于人微血管内皮细胞（HMEC）培养的合适浓度范围是100-300 μg/ml，加入肝素（50 μg/ml）后的合适浓度范围是10-20 μg/ml。ECGF pro 1因子和牛ECGF pro 2因子对小鼠细胞，牛细胞和人细胞起作用。

*每瓶中含有足够多的生长因子添加物和VEGF-A或VEGF-C，可以为血液或者淋巴内皮细胞提供合适生长环境。  

艾美捷Relia Tech内皮细胞生长添加剂相关研究：

PD-ECGF (101-M603)

ECGF (Crude Extract, cell culture grade) (300-092)

ECGF (Crude Extract, cell culture grade) + Heparin...

ECGFpro2 (complete for lymphatic ECs) (300-090-L)

ECGFpro2 + Heparin (complete for lymphatic ECs)

ECGFpro1 + Heparin (complete for blood ECs)

来源：https://www.amyjet.com/featured/ECGF.shtml