艾美捷CMRL-1066培养基参数说明及文献参考

AppearanceLight pink to orange, homogeneous, free flowing powder

SolubilityYellow to orange, clear, complete

pH3.4 ± 0.5

Endotoxin≤1EU/ml

Directions per LiterDissolve 9.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage ａnd StabilityStore powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly ａnd kept in a dark, low humidity environment. Prepared media should be kept at 4°C ａnd used within a short period of time.

CMRL培养基富含核酸和维生素，早被开发用来培养猴子的肾细胞和L-cell，适用于人和猴及其他哺乳动物细胞的培养。

接下来咱们一起看看艾美捷CMRL-1066培养基相关参数说明~

艾美捷CMRL-1066培养基基本参数：

英文名称：C5900-01 CMRL1066 w/o L-Glutamine (Powder)

外观：淡粉色至橙色，均匀，自由流动的粉末

溶解度：黄色至橙色，清澈，完全

pH：3.4±0.5

内毒素：≤1单位/毫升

每升说明：将9.7g溶解在800-900ml ddH2O中，轻轻搅拌直至完全溶解。搅拌时加入2.2g碳酸氢钠。将培养基的pH值调整至低于所需水平的0.1-0.3 pH单位。添加额外的水，使溶液进入1L过滤器，使用0.22微米的膜过滤器进行消毒。等分到无菌容器中。不要高压釜。含有不稳定的化合物，可能会因高压灭菌而损坏。

储存和稳定性：在室温下储存粉状介质。收到后可稳定12个月。打开的瓶子应盖紧盖子，并保持在黑暗、低湿度的环境中。准备好的培养基应保持在4°C，并在短时间内使用。

艾美捷CMRL-1066培养基配方：

无机盐：

Calcium Chloride, anhydrous 0.20

Magnesium Sulfate, anhydrous 0.09769

Potassium Chloride 0.40

Sodium Acetate, anhy 0.05

Sodium Phosphate Monobasic, anhy 0.122

Sodium Chloride 6.80

氨基酸：

L-Alanine 0.025

L-Arginine 0.05787

L-Aspartic Acid 0.03

L-Cysteine•HCl•H2O 0.26

L-Cystine 0.02

L-GlutamicAcid, Sodium 0.0862

L-Glutamine Absent

Glycine 0.05

L-Histidine•HCl•H2O 0.02

trans-4-Hydroxy-L-Proline 0.01

L-Isoleucine 0.02

L-Leucine 0.06

L-Lysine•HCl 0.07

L-Methionine 0.015

L-Phenylalanine 0.025

L-Proline 0.04

L-Serine 0.025

L-Threonine 0.03

L-Tryphan 0.01

L-Tyrosine 0.04

L-Valine 0.025

维生素：

PABA 0.00005

L-Ascorbic Acid•Na 0.05

D-Biotin 0.00001

Choline Chloride 0.0005

Coenzyme A•Na 0.0025

Cocarboxylase 0.001

2＇-Deoxyadenosine 0.01

2＇-Deoxycytidine•HCl 0.0116

2＇-Deoxyguanosine 0.01

Folic Acid 0.00001

myo-Inositol 0.00005

5-Methyldeoxycytidine 0.0001

ß-NAD 0.007

ß-NADP•Na 0.001

Nicotinic Acid 0.000025

Niacinamide 0.000025

D-PantothenicAcid•Ca 0.00001

Pyridoxal•HCl 0.000025

Pyridoxine•HCl 0.000025

Riboflavin 0.00001

Flavine Adenine Dinucleotide•2Na 0.000106

Thiamine•HCl 0.00001

艾美捷CMRL-1066培养基文献参考：

1. Healy, G.M., Parker, R.C.: An Improved Chemically Defined Basal Medium (CMRL-1415) For Newly Explanted Mouse Embryo Cells. Journal of Cell Biology 30: 531-538 (1966).

2. Parker, R.C., et al.: Altered Cell Strains In Continuous Culture: A General Survey In: Special Publications of the New York Academy of Sciences 5: 303-313 (1957). Publisher: N.Y. Acad. of Sci.. Ed.: Whitelock, O.

3. Methods of Tissue Culture, Third Edition, pp. 62-80. Publisher: Harper ａnd Row Publishers Inc., New York. Ed.: Parker, R.C.

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