AAT-2559中英文说明丨艾美捷PerCP蛋白介绍

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency ａnd a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its cyanine tandem conjugates (such as PerCP-Cy5.5 developed by BD) can be excited with a standard 488 nm laser ａnd emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE ａnd other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers. AAT Bioquest offers iFluor protein labeling dyes that are generally brighter ａnd more photostable than the corresponding cyanine dyes of similar wavelengths. iFluor protein labeling dyes are superior alternatives to the cyanine dyes.

PerCP蛋白（AAT-2559）艾美捷中文说明：

PerCP（Peridinin叶绿素蛋白复合物）是从Dinophyceae sp。中分离出来的。它具有高的消光系数，高量子效率和大的斯托克斯位移。它在488 nm处被氩激光很好地激发，其大发射峰在677nm处。PerCP蛋白通常用于荧光免疫标记，特别是在涉及荧光激活细胞分选（FACS）的应用中。它的花青串联共轭物（例如BD开发的PerCP-Cy5.5）可以用标准的488 nm激光激发，并以更长的波长发射远红色，用于细胞的多色流式细胞仪分析。这些多发射波长使得PerCP-Cyanine共轭物可能是有用的荧光染料，用于FITC，PE和其他荧光染料的多色分析。PerCP串联结构可以使其比单独的PerCP更具光稳定性，PerCP通常使用更强大的水冷气体激光器快速光漂白。AAT iFluor蛋白标记染料通常比相应波长相似的花青染料更亮，更具光稳定性。iFluor蛋白标记染料是花青染料的优良替代品。

PerCP蛋白基本参数：

中文名称：PerCP(多甲藻黄素-叶绿素-蛋白质 复合物)

英文名字：PerCP(Peridinin-Chlorophyll-Protein Complex)

规格：1mg

形式：固体形式

保存建议：在低于2-4℃温度下冷藏保存，要求储存环境干燥并避免光照

分子量35000

溶剂水

冷藏（2-8°C）；尽量减少曝光

图：Peridinin叶绿素（PerCP）是一些生物在光合作用中使用的类胡萝卜素色素。许多光合作用的甲藻使用peridinin，其吸收470-550nm范围内的蓝绿光，超出叶绿素分子可接近的范围。

艾美捷peridinin-叶绿素-蛋白质复合物是一种特殊的分子复合物，由船形蛋白质分子组成，具有大的中心腔，含有peridinin，叶绿素和脂质分子，通常以peridinin与叶绿素的4:1比例。PerCP通常用于免疫测定，例如荧光激活细胞分选（FACS）和流式细胞术。荧光团与用于研究应用的蛋白质或抗体共价连接。

艾美捷PerCP蛋白相关文献：

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来源：https://www.amyjet.com/products/AAT-2559.shtml