中英文说明丨艾美捷017-000-121正常驴血清介绍

Information

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, ａnd freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Usage

Physical State: Freeze-dried solid

Storage ａnd Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) ａnd centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot ａnd freeze undiluted product at -20°C or below. Avoid repeated freezing ａnd thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.

017-000-121正常驴血清（艾美捷）中文说明：

正常血清经脂质提取以提高透明度，用含叠氮化钠的磷酸盐缓冲盐水（PBS）透析，然后冷冻干燥。强烈推荐在PBS中稀释至5%（v/v）的正常血清作为阻断试剂，以减少非特异性、保守序列和/或Fc受体结合的背景。在添加一抗体之，使用来自与标记抗体相同的宿主的稀释的正常血清作为单独的孵育步骤获得佳结果。

艾美捷017-000-121正常驴血清基本参数：

Host: Donkey

Product:

Conjugate: Unconjugated

Product Category: Normal Serums ａnd Gamma Globulins

RRID: AB_2337258

注意：

1、017-000-121正常驴血清可应用于大多数免疫学实验，用作封闭液或空白对照.

2、冻干粉末形式，保存在2-8°C，准备用时，根据指示的体积加入双蒸水，在2-8°C条件下，未稀释的液体保质期6周，每天准备新鲜的工作液.如果重溶后，想延长保存期，将未稀释的溶液保存在-20°C或者更低，避免反复冻融.

来源：https://www.amyjet.com/products/017-000-121.shtml