应用广泛灵敏丨艾美捷12(S)-HETE-ELISA试剂盒定量方案

12(S)-HETE-ELISA试剂盒化学性质：

检测方法：

Colorimetric

精确度：

批次内

样品 n Mean SD CV%

Buffer 16 342pg/ml  = 5.2%

Buffer 16 1153pg/ml  = 10.1%

Buffer 16 4762pg/ml  = 15.5%

批次间

样品 n Mean SD CV%

Buffer  224pg/ml  = 4.1%

Buffer  1127pg/ml  = 9.1%

Buffer  5294pg/ml  = 20.8%

样品类型：

Cell culture supernatant, Plasma

检测类型：

Competitive

灵敏度：

= 146.3 pg/ml

范围：

195 pg/ml - 50000 pg/ml

回收率：

特定样本回收率

样品类型 平均% 范围

Cell culture media = 94 % - %

Hep Plasma = 104 % - %

EDTA Plasma = 97 % - %

实验步骤：

Multiple steps standard assay

12(S)-HETE-ELISA试剂盒是一种比色竞争性酶免疫测定试剂盒，5小时内即可获得结果。在405 nm处读取吸光度。使用易于使用的液体色码试剂可获得快速、准确和可靠的结果，从而减少误差。

12(S)-HETE-ELISA试剂盒ADI-900-050文献参考：

Leukotriene B4 receptor-2 contributes to KRAS-driven lung tumor formation by promoting interleukin-6-mediated inflammation: J.H. Jang, et al.; Exp. Mol. Med. 53, 1559 (2022), Abstract;

Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function: M. Otto, et al.; Life Sci. 284, 119925 (2021), Abstract;

12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1: M. Otto, et al.; J. Clin. Invest. 130, 4999 (2020)

来源：https://www.amyjet.com/products/ADI-900-050.shtml