附文献，艾美捷一氧化氮(总)检测试剂盒方案

艾美捷一氧化氮（总）检测试剂盒（ADI-917-020）基于硝酸还原酶将硝酸盐酶促转化为亚硝酸盐，然后进行格里斯反应以形成有色偶氮染料产物。通过测量540-570nm处的吸收进行定量。每孔仅需 0.050 ml 稀释样品，可节省宝贵的样品。灵敏、高效、简便的总一氧化氮检测方法，只需40应用。

 一氧化氮(总)检测试剂盒基参：

Sensitivity: 0.625 µmol/L (range 3.125 - 100 µmol/L)

Applications: Colorimetric detection

Application Notes: For the quantitative determination of total Nitric Oxide in culture supernatants, plasma, serum, saliva, ａnd urine from any species. Cited sample type includes tissue (ref).

Species reactivity: Species independent

Use/Stability: Store all components at 4°C, except nitrate reductase at -20°C.

Shipping: Shipped on Blue Ice

Contents: Microtiter plate, Nitrate Reductase, Nitrate Reductase Storage Buffer, NADH, Nitrate Standard, Reaction buffer concentrate, Griess Reagent I, Griess Reagent II

一氧化氮(总)检测试剂盒（ADI-917-020）特点：

高效 - 仅需 50 μl 稀释样品，节省珍贵样品

灵敏 - 检测低至 0.625 μmol/L

值得信赖 - 在同行评审的出版物中被广泛引用

快速 - 在短短 80 分钟内从多达 40 个样品中得到一式两份的结果

一氧化氮(总)检测试剂盒部分文献参考：

神经元NOS激活脊髓NADPH氧化酶2促进小鼠中枢Sigma-1受体诱导的疼痛超敏反应：S.R.Choi等人。；生物制药公牛。391922（2016）；

Hepatic ischemia/reperfusion injury associates with acute kidney injury in liver transplantation: prospective cohort study: I. Jochmans, et al.; Liver Transpl. 23, 634 (2017), Application(s): ELISA using human urine;

在华支睾吸虫感染期间，过氧化物酶6的表达与核因子-κB的激活呈负相关：J.H.Pak等人。；自由基。Biol.Med.99273（2016），应用：测量培养基中的亚硝酸盐/硝酸盐浓度；

Urinary calprotectin, kidney injury molecule-1, ａnd neutrophil gelatinase-associated lipocalin for the prediction of adverse outcome in pediatric acute kidney injury: J.H. Westhoff, et al.; Eur. J. Pediatr. 176, 745 (2017), Application(s): ELISA using human urine;

用于主动脉瓣共培养模型的三维空间组织PEG基水凝胶：D.S.Puperi等人。；生物材料67，354（2015），应用：培养上清液中的一氧化氮水平；

Acute kidney injury prediction in cardiac surgery patients by a urinary peptide pattern: a case-control validation study: J. Metzger, et al.; Crit. Care 20, 157 (2016), Application(s): ELISA using human urine,

来源：https://www.amyjet.com/products/ADI-917-020.shtml