BioXCell BE0101体内单克隆抗小鼠PD-L1(B7-H1)说明书

The 10F.9G2 monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells ａnd endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 ａnd CD8 thymocytes as well as activated T ａnd B lymphocytes ａnd myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation ａnd cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors ａnd results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 ａnd PD-1. The 10F.9G2 antibody has been shown to block the interaction between PD-L1 ａnd PD-1 ａnd between PD-L1 ａnd B7-1 (CD80).

BioXCell 艾美捷BE0101体内单克隆抗小鼠PD-L1(B7-H1)抗体:

10F.9G2单克隆抗体与小鼠PD-L1（程序性死亡配体1）反应，也称为B7-H1或CD274。PD-L1是一种40 kDa的I型跨膜蛋白，属于Ig超家族的B7家族。PD-L1在T淋巴细胞，B淋巴细胞，NK细胞，树突状细胞以及IFNγ刺激的单核细胞，上皮细胞和内皮细胞上表达。PD-L1与其受体PD-1结合，PD-4存在于CD8和CD1胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上。

BioXCell艾美捷体内单克隆抗小鼠PD-L1(B7-H1)抗体规格：

#  BXC-BE0101

Isotype Rat IgG2b, κ

Recommended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin

Recommended Dilution Buffer InVivoPure pH 6.5 Dilution Buffer

Immunogen Mouse CD274

Reported Applications in vivo PD-L1 blockade

Immunofluorescence

Immunohistochemistry (frozen)

Flow cytometry

Western blot

Formulation PBS, pH 6.5

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

RRID AB_10949073

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 大鼠IgG2b， κ

推荐的同种型对照 体内MAb大鼠IgG2b同种型对照，抗锁孔帽贝血蓝蛋白

推荐的稀释缓冲液 体内纯 pH 6.5 稀释缓冲液

免疫原 鼠标 CD274

报告的应用程序 体内PD-L1阻断

免疫荧光

免疫组化（冷冻）

流式细胞术

蛋白质印迹

配方 PBS，pH 6.5

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

里德 AB_10949073

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-BE0101-1mg.shtml